US6740518B1
Expired / Lapsed
US
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Signal detection techniques for the detection of analytes
Assignee: Roche Molecular Systems Inc
•
Filing: 1999-09-17
•
Granted: 2004-05-25
•
Expires: 2019-09-17
Abstract
Abstract The invention relates to the use of signal processing methods in order to acheive higher signal to noise ratios, to increase the detection limits of target analytes. These techniques include the monitoring of the output signal at higher harmonic frequencies.
Inventors
- 👤 Hau H. Duong
- 👤 Stephen D. O'Connor
- 👤 Robert H. Terbrueggen
- 👤 Jon Faiz Kayyem
- 👤 Gary T. Olsen
- 👤 Daniel A. Litvack
- 👤 Javier Gonzalez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 19:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; G01N27/00; G01N27/26; G01N27/416; G01N27/28; G01N27/30; G01N27/327; G01N27/3275; G01N27/3277; B; B82; B82Y; B82Y15/00; B82Y30/00
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
114 words
A method of determining the presence of target analytes in a sample comprising: a) applying said sample to an array comprising a plurality of electrodes, wherein at least one electrode comprises an assay complex comprising: i) a capture binding ligand covalently attached to said electrode; ii) a target analyte; and iii) an electron transfer moiety; b) applying an input waveform to said electrode to generate an output waveform comprising at least one harmonic component, having a harmonic number greater than or equal to two; c) detecting said output waveform at said electrode; d) analyzing said harmonic component with harmonic number greater than or equal to two to determine the presence of said target analytes.
Claim 2
Independent Claim
114 words
A method of determining the presence of target analytes in a sample comprising: a) applying said sample to an array comprising a plurality of electrodes, wherein at least one electrode comprises an assay complex comprising: i) a capture binding ligand covalently attached to said electrode; ii) a target analyte; and iii) an electron transfer moiety; b) applying an input waveform to said electrode to generate an output waveform comprising at least one harmonic component, having a harmonic number greater than or equal to two; c) detecting said output waveform at said electrode; d) analyzing said harmonic component with harmonic number greater than or equal to two to determine the presence of said target analytes.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein said target analyte is a nucleic acid.
Claim 4
Dependent Claim
13 words
A method according to claim 1 wherein said target analyte is a protein.
Claim 5
Dependent Claim
18 words
A method according to claim 1 , wherein said analyzing comprises the use of a peak recognition scheme.
Claim 6
Dependent Claim
14 words
A method according to claim 1 , wherein said analyzing comprises a digital filter.
Claim 7
Dependent Claim
17 words
The method of claim 1 , wherein said electrode has an asymmetrical response to said input waveform.
Claim 8
Dependent Claim
13 words
The method of claim 6 , wherein said electron transfer moiety is degradable.
Claim 9
Dependent Claim
13 words
The method of claim 7 , wherein said electron transfer moiety is luminol.
Claim 10
Dependent Claim
14 words
The method of claim 6 , further comprising adding a co-reductant to said sample.
Claim 11
Dependent Claim
11 words
The method of claim 9 , wherein said co-reductant is ferrocyanide.
Claim 12
Dependent Claim
19 words
The method of claim 9 , wherein said co-reductant has a lower redox potential than said electron transfer moiety.
Claim 13
Dependent Claim
14 words
The method of claim 6 , further comprising adding a co-oxidant to said sample.
Claim 14
Dependent Claim
57 words
The method of claim 1 , wherein said input waveform is a voltage waveform and said output waveform is a current waveform, wherein said input waveform comprises an AC component having a first frequency and a first amplitude, and wherein said first amplitude is selected such that said output waveform comprises at least one non-linear harmonic component.
Claim 15
Dependent Claim
33 words
The method of claim 1 , wherein said harmonic component is chosen from the group of harmonic components consisting of a second, third, fourth, fifth, sixth, seventh, eighth, ninth, and tenth harmonic components.
Claim 16
Dependent Claim
26 words
The method of claim 1 , wherein the output waveform comprises a plurality of harmonic components and said method comprises analyzing the plurality of harmonic components.
Claim 17
Dependent Claim
18 words
The method of claim 1 , wherein said input waveform is a voltage waveform comprising a square wave.
Claim 18
Dependent Claim
15 words
The method of claim 16 , wherein said harmonic component is an even harmonic component.
Claim 19
Dependent Claim
17 words
The method of claim 1 , comprising computing a fast fourier transform of said detected output waveform.
Claim 20
Dependent Claim
18 words
The method of claim 1 , further comprising computing a joint time-frequency transform of said detected output waveform.
Claim 21
Dependent Claim
20 words
The method of claim 1 , wherein said input waveform comprises a plurality of components, each having a different frequency.
Claim 22
Dependent Claim
36 words
The method of claim 1 , further comprising fitting said harmonic component to a first curve and a second curve, wherein said first curve describes a Faradaic signal and said second curve describes a background signal.
Claim 23
Dependent Claim
21 words
The method of claim 21 , wherein said first curve is based, at least in part, on a modified Gaussian distribution.
Claim 24
Dependent Claim
15 words
The method of claim 21 , wherein said second curve is a fifth order polynomial.
Claim 25
Dependent Claim
15 words
The method of claim 21 , wherein said fitting comprises minimizing a mean square error.
Claim 26
Dependent Claim
14 words
The method of claim 23 , wherein said fitting comprises using singular value decomposition.
Claim 27
Dependent Claim
12 words
The method of claim 1 , wherein said analyzing comprises digital filtering.
Claim 28
Dependent Claim
28 words
The method of claim 26 , wherein said filtering utilizes a filter chosen from the group consisting of a match filter, a weiner filter, and a Kalman filter.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2007-05-25 | 2007-11-24 | 2008-05-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2011-05-25 | 2011-11-24 | 2012-05-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2015-05-25 | 2015-11-24 | 2016-05-25 | $7700.00 | Window Closed / Lapsed |