US6096825A
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Electrically conductive electroactive functionalized conjugated polymers, and uses thereof
Assignee: Biomerieux SA
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Filing: 1995-04-24
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Granted: 2000-08-01
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Expires: 2015-04-24
Abstract
Abstract There are electrically conductive, electroactive functionalized conjugated polymers having formula (I): ##STR1## These electrically conducive, electroactive conjugated polymers may be covalently bonded to a first biological molecule or antiligand. Polymers bonded to a first biological molecule or antiligand may be used to form an electrode and may be used to assay for, detect and/or extract a second biological molecule or ligand.
Inventors
- 👤 Francis Garnier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; C; C08; C08G; C08G61/00; C08G61/12; C08G61/122; C08G61/123; C08G61/124; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6825; H; H01; H01B; H01B1/00; H01B1/06; H01B1/12; H01B1/124; H01B1/127; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816; Y10S530/817
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
100 words
An electrically conductive, electroactive conjugated polymer of formula (II): ##STR7## wherein n is a non-zero integer and i is an integer ranging from 2 to n-1, and each R' of R' 1 , R' i and R' n , which may be identical or different, represents H or a functional group capable of covalently bonding with, or covalently bonded to, a first biological molecule or an antiligand, provided that at least one said R' represents a functional group that is covalently bonded to a first biological molecule or antiligand, wherein said first biological molecule comprises a polynucleotide or peptide sequence.
Claim 2
Dependent Claim
100 words
An electrically conductive, electroactive conjugated polymer of formula (II): ##STR7## wherein n is a non-zero integer and i is an integer ranging from 2 to n-1, and each R' of R' 1 , R' i and R' n , which may be identical or different, represents H or a functional group capable of covalently bonding with, or covalently bonded to, a first biological molecule or an antiligand, provided that at least one said R' represents a functional group that is covalently bonded to a first biological molecule or antiligand, wherein said first biological molecule comprises a polynucleotide or peptide sequence.
Claim 3
Dependent Claim
104 words
The polymer as claimed in claim 1, wherein each said functional group, before being bonded to a biological molecule or antiligand, is selected from the group consisting of: Y p --C--X, where X represents H, OH, a substituted or unsubstituted lower O-alkyl radical, or a halogen; Y p --NHZ, where Z represents H or an alkyl radical; Y p --NH--CO--CF 3 ; Y p --X, where X represents H, OH, a substituted or unsubstituted lower O-alkyl radical, or a halogen; --Si (alkyl) 3 ; --Si (alkoxyl) 3 ; and an activated ester group; wherein p is an integer less than or equal to three.
Claim 4
Dependent Claim
68 words
The polymer as claimed in claim 2, wherein Y is selected from the group consisting of: alkyls having from 1 to 5 carbon atoms, alkoxyls having from 1 to 5 carbon atoms, and polyethers corresponding to the formula (CH 2 --CH 2 --O) m --(CH 2 ) m' --, m representing an integer ranging from 1 to 3 and m' representing an integer equal to 1 or 2.
Claim 5
Dependent Claim
32 words
The polymer as claimed in claim 1, wherein each said functional group bonded to a biological molecule or antiligand, before reaction with the first biological molecule or antiligand, is --(CH 2 )--COOH.
Claim 6
Dependent Claim
26 words
The conjugated polymer as claimed in claim 1, wherein each said first biological molecule is selected from the group consisting of: peptides, peptide derivatives and polynucleotides.
Claim 7
Dependent Claim
15 words
An electrode comprising a conductive substrate and a polymer as defined in any claim 1.
Claim 8
Dependent Claim
14 words
A self-supporting film of a polymer comprising a polymer as defined in claim 1.
Claim 9
Dependent Claim
22 words
The polymer as claimed in claim 2, wherein p is an integer selected from the group consisting of 0, 1 and 2.
Claim 10
Dependent Claim
14 words
The polymer as claimed in claim 2, wherein the activated ester group is N-hydroxysuccinimide.
Claim 11
Dependent Claim
12 words
The polymer as claimed in claim 2, wherein the halogen is chlorine.
Claim 12
Dependent Claim
22 words
The conjugated polymer as claimed in claim 5, wherein the peptide is selected from the group consisting of Gly-Phe, Phe-Pro and Phe-Hea-Pro.
Claim 13
Dependent Claim
13 words
The conjugated polymer as claimed in claim 5, wherein the polynucleotide is CCTAAGAGGGAGTG.
Claim 14
Dependent Claim
84 words
A method for at least one of detecting, assaying and extracting a second biological molecule or ligand in an in vitro or in vivo solution, comprising: bringing a conjugated polymer as claimed in claim 1, which contains at least one said first biological molecule or antiligand that is different from and specifically interacts with said second biological molecule or ligand, into contact with said solution; and at least one of determining whether the specific interaction occurs and extracting said second biological molecule or ligand.
Claim 15
Dependent Claim
58 words
The method of claim 13, wherein said second biological molecule or ligand is detected or assayed by at least one of observation and measurement of a potential difference or a variation in current between the conjugated polymer not bonded to the second biological molecule or ligand and the conjugated polymer bonded to the second biological molecule or ligand.
Claim 16
Dependent Claim
17 words
The method as claimed in claim 13, wherein the second biological molecule or ligand is an enzyme.
Claim 17
Dependent Claim
17 words
The method as claimed in claim 13, wherein the second biological molecule or ligand is a polynucleotide.
Claim 18
Dependent Claim
26 words
The method as claimed in claim 13, wherein the conjugated polymer is deposited on a conductive substrate or is in the form of a self-supporting film.
Claim 19
Dependent Claim
14 words
The method of claim 13, wherein said process determines whether said specific interaction occurs.
Claim 20
Dependent Claim
14 words
The method of claim 13, wherein said second biological molecule or ligand is extracted.
Claim 21
Dependent Claim
19 words
The conjugated polymer as claimed in claim 1, wherein said antiligand is able to form an antiligand/target molecule complex.
Claim 22
Dependent Claim
27 words
The conjugated polymer as claimed in claim 20, wherein said complex is selected from the group consisting of peptide/antibody, antibody/haptene, hormone/receptor, polynucleotide hybrids/polynucleotide and polynucleotide/nucleic acid couple.
References Cited (Backward Citations)
EP0157526A2
Cited
FR2607507A1
Cited
EP0314009A2
Cited
WO1990010655A1
Cited
FR2656868A1
Cited
US5149826A
Cited
WO1993006237A1
Cited
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