US6258545B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Nucleic acid mediated electron transfer
Assignee: California Institute of Technology
•
Filing: 2000-04-07
•
Granted: 2001-07-10
•
Expires: 2020-04-07
Abstract
Abstract The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Inventors
- 👤 Thomas J. Meade
- 👤 Jon Faiz Kayyem
- 👤 Scott E. Fraser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:46
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6825; C07; C07H; C07H21/00; C07H23/00; C12Q1/6818
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
78 words
A method for detecting a target sequence comprising: a) performing a PCR reaction to form an amplified target sequence; b) hybridizing said amplified target sequence to a single-stranded nucleic acid covalently attached to an electrode to form a hybridization complex, wherein said hybridization complex comprises at least one moiety capable of donating or accepting an electron; c) detecting electron transfer between said moiety and said electrode as an indication of the presence or absence of said target sequence.
Claim 2
Independent Claim
78 words
A method for detecting a target sequence comprising: a) performing a PCR reaction to form an amplified target sequence; b) hybridizing said amplified target sequence to a single-stranded nucleic acid covalently attached to an electrode to form a hybridization complex, wherein said hybridization complex comprises at least one moiety capable of donating or accepting an electron; c) detecting electron transfer between said moiety and said electrode as an indication of the presence or absence of said target sequence.
Claim 3
Dependent Claim
37 words
A method according to claim 1 wherein said PCR reaction comprises hybridizing at least one PCR primer to said target sequence, wherein said PCR primer comprises at least one moiety capable of donating or accepting an electron.
Claim 4
Dependent Claim
48 words
A method according to claim 1 wherein said PCR reaction comprises: a) hybridizing at least one PCR primer to said target sequence; b) providing nucleosides comprising a moiety capable of donating or accepting an electron; c) incorporating said nucleosides into said amplified target sequence during said PCR reaction.
Claim 5
Dependent Claim
18 words
A method according to claim 1 , 2 or 3 wherein said moiety comprises a transition metal complex.
Claim 6
Dependent Claim
13 words
A method according to claim 4 wherein said transition metal complex comprises ruthenium.
Claim 7
Dependent Claim
13 words
A method according to claim 4 wherein said transition metal complex comprises osmium.
Claim 8
Dependent Claim
13 words
A method according to claim 4 wherein said transition metal complex comprises iron.
Claim 9
Dependent Claim
13 words
A method according to claim 4 wherein said transition metal complex comprises cobalt.
Claim 10
Dependent Claim
19 words
A method according to claim 1 , 2 or 3 wherein said moiety comprises an organic electron transfer moiety.
Claim 11
Dependent Claim
20 words
A method according to claim 1 , 2 or 3 wherein said moiety comprises a covalently attached electron transfer moiety.
Claim 12
Dependent Claim
16 words
A method according to claim 10 wherein said electron transfer moiety is a transition metal complex.
Claim 13
Dependent Claim
13 words
A method according to claim 11 wherein said transition metal complex comprises ruthenium.
Claim 14
Dependent Claim
13 words
A method according to claim 11 wherein said transition metal complex comprises osmium.
Claim 15
Dependent Claim
13 words
A method according to claim 11 wherein said transition metal complex comprises iron.
Claim 16
Dependent Claim
13 words
A method according to claim 11 wherein said transition metal complex comprises cobalt.
Claim 17
Dependent Claim
21 words
A method according to claim 1 , 2 or 3 wherein said moiety comprises a covalently attached organic electron transfer moiety.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-07-09 | 2005-01-08 | 2005-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-07-09 | 2009-01-08 | 2009-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-07-09 | 2013-01-08 | 2013-07-10 | $7700.00 | Window Closed / Lapsed |