US6071699A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Nucleic acid mediated electron transfer
Assignee: California Institute of Technology
•
Filing: 1998-06-19
•
Granted: 2000-06-06
•
Expires: 2018-06-19
Abstract
Abstract The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Inventors
- 👤 Thomas J. Meade
- 👤 Jon Faiz Kayyem
- 👤 Scott E. Fraser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 09:17
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6825; C07; C07H; C07H21/00; C07H23/00; C12Q1/6818
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
97 words
A method of detecting a target nucleic acid sequence in a sample comprising: a) initiating electron transfer by applying an initiation signal to a hybridization complex comprising said target sequence, if present, hybridized to a single-stranded nucleic acid probe covalently attached to an electrode, wherein at least one of said probe or target sequence comprises a moiety capable of donating or accepting an electron; and b) detecting electron transfer between said moiety which donates or accepts an electron and said electrode to distinguish between single stranded and double stranded nucleic acid to detect said target nucleic acid.
Claim 2
Independent Claim
97 words
A method of detecting a target nucleic acid sequence in a sample comprising: a) initiating electron transfer by applying an initiation signal to a hybridization complex comprising said target sequence, if present, hybridized to a single-stranded nucleic acid probe covalently attached to an electrode, wherein at least one of said probe or target sequence comprises a moiety capable of donating or accepting an electron; and b) detecting electron transfer between said moiety which donates or accepts an electron and said electrode to distinguish between single stranded and double stranded nucleic acid to detect said target nucleic acid.
Claim 3
Dependent Claim
21 words
A method according to claim 1 wherein said probe nucleic acid comprises said moiety capable of donating or accepting an electron.
Claim 4
Dependent Claim
21 words
A method according to claim 1 wherein said target nucleic acid comprises said moiety capable of donating or accepting an electron.
Claim 5
Dependent Claim
24 words
A method according to claim 1 wherein a base of said nucleic acid probe comprises said moiety capable of donating or accepting an electron.
Claim 6
Dependent Claim
15 words
A method according to claim 1 wherein said nucleic acid probe comprises a ribose-phosphate backbone.
Claim 7
Dependent Claim
24 words
A method according to claim 5 wherein a ribose of said nucleic acid probe comprises said moiety capable of donating or accepting an electron.
Claim 8
Dependent Claim
24 words
A method according to claim 5 wherein a phosphate of said nucleic acid probe comprises said moiety capable of donating or accepting an electron.
Claim 9
Dependent Claim
16 words
A method according to claim 1 wherein said nucleic acid probe comprises a nucleic acid analog.
Claim 10
Dependent Claim
15 words
A method according to claim 8 wherein said nucleic acid analog comprises peptide nucleic acid.
Claim 11
Dependent Claim
21 words
A method according to claim 1 wherein said moiety capable of donating or accepting an electron is a transition metal complex.
Claim 12
Dependent Claim
28 words
A method according to claim 10 wherein said transition metal complex comprises a metal ion selected from the group consisting of ruthenium, rhenium, osmium, platinum, copper or iron.
Claim 13
Dependent Claim
18 words
A method according to claim 1 wherein an electron transport mediator is utilized in detecting said electron transfer.
References Cited (Backward Citations)
US5328824A
Cited
US5449767A
Cited
US5476928A
Cited
EP0063879A2
Cited
US4711955A
Cited
US5241060A
Cited
US4994373A
Cited
US4840893A
Cited
US4849513A
Cited
US5175269A
Cited
US4707440A
Cited
US4707352A
Cited
US4943523A
Cited
US4952685A
Cited
US5002885A
Cited
US5013831A
Cited
US4894325A
Cited
US4755458A
Cited
EP0229943B1
Cited
US4868103A
Cited
EP0234938A2
Cited
JPS63238166A
Cited
US5082830A
Cited
WO1990005732A1
Cited
US5278043A
Cited
WO1992010757A1
Cited
EP0515615A1
Cited
WO1993010267A1
Cited
US5565552A
Cited
CA2090904A1
Cited
US5573906A
Cited
WO1993022678A2
Cited
WO1993023425A1
Cited
JPH0641183A
Cited
US5495908A
Cited
US5312527A
Cited
US5472881A
Cited
EP0599337A2
Cited
US5403451A
Cited
WO1995015971A2
Cited
US5770369A
Cited
US5591578A
Cited
US5824473A
Cited
US5780234A
Cited
US5705348A
Cited
US5620850A
Cited
US5601982A
Cited
WO1996040712A1
Cited
WO1997001646A2
Cited
WO1997044651A1
Cited
WO1998035232A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-06-06 | 2003-12-06 | 2004-06-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-06-06 | 2007-12-06 | 2008-06-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-06-06 | 2011-12-06 | 2012-06-06 | $7700.00 | Window Closed / Lapsed |