US6322979B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Molecular recognition at surfaces derivatized with self-assembled monolayers
Assignee: Harvard University
•
Filing: 1999-04-21
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Granted: 2001-11-27
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Expires: 2019-04-21
Abstract
Abstract An article suitable for use as a biosensor includes a molecule of a formula X-R-Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
Inventors
- 👤 Cynthia C. Bamdad
- 👤 George B. Sigal
- 👤 Jack L. Strominger
- 👤 George M. Whitesides
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:22
CPC Classifications
C; C07; C07H; C07H21/00; C07H21/04; B; B82; B82Y; B82Y15/00; B82Y30/00; B82Y40/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; C12Q1/6837; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; G01N33/54366; G01N33/54373; G01N33/551; G01N33/553; G01N2610/00
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
107 words
A method for capturing a biological binding partner of a nucleic acid strand in a sample, said method comprising: a) providing a surface comprising: i) a first species having a formula X—R—NA, wherein X represents a functional group that adheres to said surface, R represents a spacer moiety that promotes formation of a self-assembled monolayer of a plurality of said species, and NA represents a nucleic acid strand; and ii) a second species that will form a mixed self-assembled monolayer with said first species; and b) contacting said surface with a medium containing a biological molecule and allowing the molecule to bind to the nucleic acid strand.
Claim 2
Independent Claim
107 words
A method for capturing a biological binding partner of a nucleic acid strand in a sample, said method comprising: a) providing a surface comprising: i) a first species having a formula X—R—NA, wherein X represents a functional group that adheres to said surface, R represents a spacer moiety that promotes formation of a self-assembled monolayer of a plurality of said species, and NA represents a nucleic acid strand; and ii) a second species that will form a mixed self-assembled monolayer with said first species; and b) contacting said surface with a medium containing a biological molecule and allowing the molecule to bind to the nucleic acid strand.
Claim 3
Dependent Claim
12 words
A method according to claim 1 further comprising determining the biological molecule.
Claim 4
Dependent Claim
13 words
A method according to claim 2 wherein said determining is done via spectroscopy.
Claim 5
Dependent Claim
16 words
A method according to claim 1 wherein said nucleic acid strand is a single-stranded nucleic acid.
Claim 6
Dependent Claim
23 words
A method according to claim 4 wherein said biological molecule is a second nucleic acid strand that is a binding partner of NA.
Claim 7
Dependent Claim
16 words
A method according to claim 1 wherein said nucleic acid strand is a double-stranded nucleic acid.
Claim 8
Dependent Claim
14 words
A method according to claim 1 wherein said second species has the formula X—R.
Claim 9
Dependent Claim
27 words
A method according to claim 1 wherein said second species has the formula X—R—O—(—CH 2 —CH 2 —O) n —H, wherein n is from 1 to 10.
Claim 10
Dependent Claim
14 words
A method according to claim 1 wherein X is a sulfur containing functional group.
Claim 11
Dependent Claim
20 words
A method according to claim 1 wherein R is a hydrocarbon chain optionally interrupted by at least one hetero group.
Claim 12
Dependent Claim
25 words
A method according to claim 7 wherein R has the formula —(CH 2 ) n —, wherein n is from about 8 to about 24.
Claim 13
Dependent Claim
11 words
A method according to claim 1 wherein said surface comprises gold.
Claim 14
Dependent Claim
22 words
A method according to claim 1 wherein said species has the formula X—R—NA—NAB, wherein NAB represents a biological binding partner of NA.
Claim 15
Dependent Claim
21 words
A method according to claim 1 wherein said second species has a formula X—R—NSBi, wherein NSBi is a non-specific binding inhibitor.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-11-26 | 2005-05-28 | 2005-11-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-11-26 | 2009-05-28 | 2009-11-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-11-26 | 2013-05-28 | 2013-11-27 | $7700.00 | Window Closed / Lapsed |