US5294369A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ligand gold bonding
Assignee: Akzo NV
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Filing: 1990-12-05
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Granted: 1994-03-15
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Expires: 2010-12-05
Abstract
Abstract Gold sol coated with alkanethiols and alkanethiol derivatives, which provide groups on the sol available for the linking of binding moieties such as antibodies, antigens or ligands to the gold sol. Di- and tri-thiol compounds bound to gold sol also facilitate the adsorption of antibodies, antigens or ligands to the sol. The coating process, and test kits incorporating the coated sols are also included.
Inventors
- 👤 Brian L. Shigekawa
- 👤 Yung-Ao Hsieh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 21:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; B; B01; B01J; B01J13/00; G01N33/543; G01N33/54393; G01N33/551; G01N33/553; G01N33/58; G01N33/585; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/805; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
21 words
Microparticles comprising gold sol coated with at least one compound selected from the group consisting of alkanethiol, di-thiol and tri-triol compounds.
Claim 2
Dependent Claim
21 words
Microparticles comprising gold sol coated with at least one compound selected from the group consisting of alkanethiol, di-thiol and tri-triol compounds.
Claim 3
Dependent Claim
11 words
Microparticles according to claim 1, wherein said compound is an alkanethiol.
Claim 4
Dependent Claim
32 words
Microparticles according to claim 1, wherein said alkanethiol is a n-alkanethiol of the formula CH 3 (CH 2 ) n SH, and n equals a whole number from 9 to 23 inclusive.
Claim 5
Dependent Claim
12 words
Microparticles according to claim 1, wherein said compound is a di-thiol compound.
Claim 6
Dependent Claim
12 words
Microparticles according to claim 1, wherein said compound is a tri-thiol compound.
Claim 7
Dependent Claim
21 words
Microparticles according to claim 1, wherein said gold sol has a diameter of from about 10 nm to about 150 nm.
Claim 8
Dependent Claim
40 words
Microparticles according to claim 1 which are resistant to salt-induced agglomeration, whereby no more than 10% of the optical density at 540 nm of a suspension of said sol is lost after 10 minutes in the presence of 1.0% NaCl.
Claim 9
Dependent Claim
15 words
Microparticles according to claim 1, wherein said microparticles are covalently linked to a binding moiety.
Claim 10
Dependent Claim
21 words
Microparticles according to claim 8, wherein the binding moiety is selected from the group consisting of proteins, carbohydrates, antigens and ligands.
Claim 11
Dependent Claim
14 words
Microparticles according to claim 8, wherein the binding moiety is a polymer or monomer.
Claim 12
Dependent Claim
26 words
Microparticles according to claim 1, additionally comprising a bridging compound which is covalently attached to said compound and to which is covalently linked a binding moiety.
Claim 13
Dependent Claim
13 words
Microparticles according to claim 1, wherein additionally a binding moiety is passively adsorbed.
Claim 14
Dependent Claim
17 words
A diagnostic kit for use in immunoassays comprising microparticles according to claim 1, and a further solution.
Claim 15
Dependent Claim
40 words
Microparticles comprising gold sol coated with a compound of the formula RCH 2 (CH 2 ) n SH, where R is selected from the group consisting of OH, COOH, CHO and NH 2 , and n is 9 to 23.
Claim 16
Dependent Claim
40 words
Microparticles comprising gold sol coated with a compound of the formula RCH 2 (CH 2 ) n SH, where R is selected from the group consisting of OH, COOH, CHO and NH 2 , and n is 9 to 23.
Claim 17
Dependent Claim
24 words
Microparticles comprising gold sol coated with a compound of the formula SHCH 2 (CH 2 ) n SH, and n is 9 to 23.
Claim 18
Dependent Claim
24 words
Microparticles comprising gold sol coated with a compound of the formula SHCH 2 (CH 2 ) n SH, and n is 9 to 23.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-03-14 | 1997-09-13 | 1998-03-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-03-14 | 2001-09-13 | 2002-03-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-03-14 | 2005-09-13 | 2006-03-15 | $7700.00 | Window Closed / Lapsed |