US5047513A
Expired / Lapsed
US
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Metal chelate resins
Assignee: Roche Diagnostics Corp
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Filing: 1990-10-15
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Granted: 1991-09-10
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Expires: 2010-10-15
Abstract
Abstract Metal chelate resins whose complexed nitrilotriacetic acid residues are bound to a carrier matrix via a spacer and which are suitable for metal chelate chromatography of proteins, especially those which contain neighboring histidines.
Inventors
- 👤 Heinz Dobeli
- 👤 Frich Hochuli
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:51
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/22; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01D15/3828; B01J; B01J20/00; B01J20/22; B01J20/223; B01J20/281; B01J20/286; B01J20/289; B01J20/30; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3265; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
29 words
A method for the purification of proteins comprising subjecting said proteins to affinity chromatography on a metal chelate resin of the formula: Carrier matrix-spacer-NH--(CH.sub.2).sub.X --CH(COOH)--N(CH.sub.2 COO.sup.-).sub.2 Ni.sup.2+ wherein X=2-4.
Claim 2
Independent Claim
29 words
A method for the purification of proteins comprising subjecting said proteins to affinity chromatography on a metal chelate resin of the formula: Carrier matrix-spacer-NH--(CH.sub.2).sub.X --CH(COOH)--N(CH.sub.2 COO.sup.-).sub.2 Ni.sup.2+ wherein X=2-4.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein the proteins contain several neighboring histidine residues.
References Cited (Backward Citations)
US4569794A
Cited
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