US4569794A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for purifying proteins and compounds useful in such process
Assignee: Eli Lilly and Co
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Filing: 1984-12-05
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Granted: 1986-02-11
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Expires: 2004-12-05
Abstract
Abstract This invention describes a process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin. Such precursor compounds are also described.
Inventors
- 👤 Michele C. Smith
- 👤 Charles Pidgeon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 23:43
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62; B; B01; B01J; B01J20/00; B01J20/22; B01J20/223; B01J20/281; B01J20/286; B01J20/30; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3265; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/22; B01J2220/00; B01J2220/40; B01J2220/49; B01J2220/50; B01J2220/54; B01J2220/58; C07K2319/00; C07K2319/20; C07K2319/50
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
21 words
A compound comprising a biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide.
Claim 2
Dependent Claim
21 words
A compound comprising a biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide.
Claim 3
Dependent Claim
20 words
Compound of claim 1, in which the immobilized metal ion chelating peptide has from 2 to about 5 amino acids.
Claim 4
Dependent Claim
27 words
Compound of claim 2, in which the immobilized metal ion chelating peptide contains at least one amino acid selected from the group consisting of histidine and cysteine.
Claim 5
Dependent Claim
20 words
Compound of claim 3, in which at least one amino acid of the immobilized metal ion chelating peptide is histidine.
Claim 6
Dependent Claim
47 words
Compound of claim 4 in which the immobilized metal ion chelating peptide has the formula His-X in which X is selected from the group consisting of -Gly-His, -Tyr, -Gly, -Trp, -Val, -Leu, -Ser, -Lys, -Phe, -Met, -Ala, -Glu, -Ile, -Thr, -Asp, -Asn, -Gln, -Arg, -Cys, and -Pro.
Claim 7
Dependent Claim
19 words
Compound of claim 5, in which X is selected from the group consisting of -Trp, -Tyr, -Gly-His, and -Phe.
Claim 8
Dependent Claim
18 words
Compound of claim 6, in which X is selected from the group consisting of -Trp, -Tyr, and -Gly-His.
Claim 9
Dependent Claim
30 words
Compound of claim 4, in which the immobilized metal ion chelating peptide has the formula Y-His in which Y is selected from the group consisting of -Gly, -Ala, and -Tyr.
Claim 10
Dependent Claim
32 words
Compound of claim 1, in which the immobilized metal ion chelating peptide is convalently linked directly or indirectly to the biologically active polypeptide or protein at the amino terminus of the latter.
Claim 11
Dependent Claim
35 words
Compound of claim 9, in which the immobilized metal ion chelating peptide is covalently linked indirectly via a selective cleavage site to the biologically active polypeptide or protein at the amino terminus of the latter.
Claim 12
Dependent Claim
16 words
Compound of claim 1, in which the immobilized metal ion chelating peptide chelates nickel (II) ion.
Claim 13
Dependent Claim
30 words
Compound of claim 1, in which the biologically active polypeptide or protein has the amino acid sequence of an insulin A-chain, an insulin B-chain, a proinsulin, or a growth hormone.
Claim 14
Dependent Claim
70 words
Process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin.
Claim 15
Dependent Claim
70 words
Process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin.
Claim 16
Dependent Claim
20 words
Process of claim 12, in which the immobilized metal ion chelating peptide has from 2 to about 5 amino acids.
Claim 17
Dependent Claim
27 words
Process of claim 14, in which the immobilized metal ion chelating peptide contains at least one amino acid selected from the group consisting of histidine and cysteine.
Claim 18
Dependent Claim
20 words
Process of claim 15, in which at least one amino acid of the immobilized metal ion chelating peptide is histidine.
Claim 19
Dependent Claim
47 words
Process of claim 16, in which the immobilized metal ion chelating peptide has the formula His-X in which X is selected from the group consisting of -Gly-His, -Tyr, -Gly, -Trp, -Val, -Leu, -Ser, -Lys, -Phe, -Met, -Ala, -Glu, -Ile, -Thr, -Asp, -Asn, -Gln, -Arg, -Cys, and -Pro.
Claim 20
Dependent Claim
19 words
Process of claim 17, in which X is selected from the group consisting of -Trp, -Tyr, -Gly-His, and -Phe.
Claim 21
Dependent Claim
18 words
Process of claim 18, in which X is selected from the group consisting of -Trp, -Tyr, and -Gly-His.
Claim 22
Dependent Claim
30 words
Process of claim 16, in which the immobilized metal ion chelating peptide has the formula Y-His in which Y is selected from the group consisting of -Gly, -Ala, and -Tyr.
Claim 23
Dependent Claim
32 words
Process of claim 13, in which the immobilized metal ion chelating peptide is covalently linked directly or indirectly to the biologically active polypeptide or protein at the amino terminus of the latter.
Claim 24
Dependent Claim
35 words
Process of claim 21, in which the immobilized metal ion chelating peptide is covalently linked indirectly via a selective cleavage site to the biologically active polypeptide or protein at the amino terminus of the latter.
Claim 25
Dependent Claim
30 words
Process of claim 13, in which the biologically active polypeptide or protein has the amino acid sequence of an insulin A-chain, an insulin B-chain, a proinsulin, or a growth hormone.
Claim 26
Dependent Claim
33 words
Process of claim 13, in which the resin containing immobilized metal ions is selected from the group consisting of chelating Sepharose 6B, Immobilized Iminodiacetic Acid I, Immobilized Iminodiacetic Acid II, and Chelex 100.
Claim 27
Dependent Claim
26 words
Process of claim 13, in which the immobilized metal ion is the divalent ion of metals selected from the group consisting of cobalt, nickel, and copper.
Claim 28
Dependent Claim
25 words
Process of claim 25, in which the immobilized metal ion is the divalent ion of metals selected from the group consisting of nickel and copper.
Claim 29
Dependent Claim
13 words
Process of claim 26, in which the immobilized metal ion is nickel (II).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1989-02-10 | 1989-08-12 | 1990-02-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-02-10 | 1993-08-12 | 1994-02-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-02-10 | 1997-08-12 | 1998-02-11 | $7700.00 | Window Closed / Lapsed |