US6180352B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Nucleic acid mediated electron transfer
Assignee: California Institute of Technology
•
Filing: 1999-12-10
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Granted: 2001-01-30
•
Expires: 2019-12-10
Abstract
Abstract The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Inventors
- 👤 Thomas J. Meade
- 👤 Jon Faiz Kayyem
- 👤 Scott E. Fraser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 02:50
CPC Classifications
C; C07; C07H; C07H21/00; C07H23/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; C12Q1/6825; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
134 words
A method of detecting the presence of a sequence in a nucleic acid sample, said method comprising: a) applying an input signal to an electrode comprising a hybridization complex, wherein said hybridization complex comprises: 1) a single stranded nucleic acid; 2) a target sequence hybridized to said single stranded nucleic acid; and 3) at least one covalently attached electron donor moiety; such that an electron is transferred from said electron donor moiety to said electrode such that the redox state of said electron donor moiety is changed to form an electron acceptor moiety; b) transferring an electron from a reductant to said electron acceptor moiety; and c) detecting electron transfer between said electron donor moiety and said electrode, wherein the presence of electron transfer is an indication of the presence of said target sequence.
Claim 2
Independent Claim
134 words
A method of detecting the presence of a sequence in a nucleic acid sample, said method comprising: a) applying an input signal to an electrode comprising a hybridization complex, wherein said hybridization complex comprises: 1) a single stranded nucleic acid; 2) a target sequence hybridized to said single stranded nucleic acid; and 3) at least one covalently attached electron donor moiety; such that an electron is transferred from said electron donor moiety to said electrode such that the redox state of said electron donor moiety is changed to form an electron acceptor moiety; b) transferring an electron from a reductant to said electron acceptor moiety; and c) detecting electron transfer between said electron donor moiety and said electrode, wherein the presence of electron transfer is an indication of the presence of said target sequence.
Claim 3
Dependent Claim
27 words
A method according to claim 1 wherein said detecting is done by detecting an output signal characteristic of electron transfer between said donor moiety and said electrode.
Claim 4
Independent Claim
134 words
A method of detecting the presence of a sequence in a nucleic acid sample, said method comprising: a) applying an input signal to an electrode comprising a hybridization complex, wherein said hybridization complex comprises: 1) a single stranded nucleic acid; 2) a target sequence hybridized to said single stranded nucleic acid; and 3) at least one covalently attached electron acceptor moiety; such that an electron is transferred from said electron acceptor moiety to said electrode such that the redox state of said electron acceptor moiety is changed to form an electron donor moiety; b) transferring an electron from an oxidant to said electron donor moiety; and c) detecting electron transfer between said electron acceptor moiety and said electrode, wherein the presence of electron transfer is an indication of the presence of said target sequence.
Claim 5
Independent Claim
134 words
A method of detecting the presence of a sequence in a nucleic acid sample, said method comprising: a) applying an input signal to an electrode comprising a hybridization complex, wherein said hybridization complex comprises: 1) a single stranded nucleic acid; 2) a target sequence hybridized to said single stranded nucleic acid; and 3) at least one covalently attached electron acceptor moiety; such that an electron is transferred from said electron acceptor moiety to said electrode such that the redox state of said electron acceptor moiety is changed to form an electron donor moiety; b) transferring an electron from an oxidant to said electron donor moiety; and c) detecting electron transfer between said electron acceptor moiety and said electrode, wherein the presence of electron transfer is an indication of the presence of said target sequence.
Claim 6
Dependent Claim
27 words
A method according to claim 3 wherein said detecting is done by detecting an output signal characteristic of electron transfer between said acceptor moiety and said electrode.
Claim 7
Dependent Claim
15 words
A method according to claim 2 or 4 wherein said output signal is a current.
Claim 8
Dependent Claim
17 words
A method according to claim 1 or 3 wherein said single stranded nucleic acid comprises said electrode.
Claim 9
Dependent Claim
19 words
A method according to claim 1 or 3 wherein said single stranded nucleic acid comprises said electron donor moiety.
Claim 10
Dependent Claim
17 words
A method according to claim 1 or 3 wherein said target sequence comprises said electron donor moiety.
Claim 11
Dependent Claim
19 words
A method according to claim 1 or 3 wherein said hybridization complex comprises a plurality of electron donor moieties.
Claim 12
Dependent Claim
18 words
A method according to claim 1 or 3 wherein said electron donor moiety is a transition metal complex.
Claim 13
Dependent Claim
27 words
A method according to claim 10 wherein said transition metal complex comprises a metal selected from the group consisting of ruthenium, rhenium, osmium, platinum, copper and iron.
Claim 14
Dependent Claim
19 words
A method according to claim 1 or 3 wherein said electron donor moiety is an organic electron donor moiety.
Claim 15
Dependent Claim
14 words
A method according to claim 1 wherein said reductant is a hexacyano iron complex.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-01-30 | 2004-07-31 | 2005-01-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-01-30 | 2008-07-31 | 2009-01-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-01-30 | 2012-07-31 | 2013-01-30 | $7700.00 | Window Closed / Lapsed |