US5356786A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Interferant eliminating biosensor
Assignee: Therasense Inc
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Filing: 1993-12-02
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Granted: 1994-10-18
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Expires: 2013-12-02
Abstract
Abstract Interferant eliminating analyte sensors and sensing process prevent erroneous assays. Glucose electrodes were coated with an oxidizing enzyme (peroxidase) which allows hydrogen peroxide to selectively oxidize ascorbate, urate, bilirubin, and acetaminophenol in the presence of glucose. Hydrogen peroxide may be added to the assayed solution or generated in situ. The oxidizing enzyme was prevented from causing undesired reduction currents at the glucose electrode by preventing contact of the oxidizing enzyme with the glucose electrode, or by increasing the applied voltage.
Inventors
- 👤 Adam Heller
- 👤 Ruben Maidan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 17:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
79 words
A biosensor comprising: an electrode on which an analyte is electrooxidized at a given applied potential; and an interferant-eliminating layer substantially covering the electrode but electrically isolated therefrom at the given applied potential, said interferant-eliminating layer comprising a catalyst which is capable of catalizing substantial oxidation of a plurality of interferants but not substantial oxidation of the analyte, wherein the catalyst mediates the following reaction: ##STR4## where AH 2 represents an interfering compound and A represents a non-interfering compound.
Claim 2
Dependent Claim
79 words
A biosensor comprising: an electrode on which an analyte is electrooxidized at a given applied potential; and an interferant-eliminating layer substantially covering the electrode but electrically isolated therefrom at the given applied potential, said interferant-eliminating layer comprising a catalyst which is capable of catalizing substantial oxidation of a plurality of interferants but not substantial oxidation of the analyte, wherein the catalyst mediates the following reaction: ##STR4## where AH 2 represents an interfering compound and A represents a non-interfering compound.
Claim 3
Dependent Claim
14 words
The biosensor of claim 1, wherein the catalyst is a natural or synthetic enzyme.
Claim 4
Dependent Claim
10 words
The biosensor of claim 2, wherein the enzyme is peroxidase.
Claim 5
Dependent Claim
43 words
The biosensor of claim 3, wherein the peroxidase is horseradish peroxidase, cytochrome c peroxidase, chloroperoxidase, lactoperoxidase, thyroid peroxidase, Japanese horseradish peroxidase a, Japanese horseradish peroxidase c, myeloperoxidase, NADH peroxidase, turnip peroxidase A 1 , turnip peroxidase B, turnip peroxidase D, or glutathione peroxidase.
Claim 6
Dependent Claim
15 words
The biosensor of claim 2, wherein the catalyst is an enzyme having iron (III) porphyrins.
Claim 7
Dependent Claim
17 words
The biosensor of claim 1, wherein an insulating barrier is positioned between said catalyst and said electrode.
Claim 8
Dependent Claim
12 words
The biosensor of claim 6, wherein said insulating barrier is a membrane.
Claim 9
Dependent Claim
15 words
The biosensor of claim 7, wherein said membrane is cellulose acetate, polycarbonate, or perfluorinated membrane.
Claim 10
Dependent Claim
14 words
The biosensor of claim 1, wherein said catalyst is immobilized within a polymeric matrix.
Claim 11
Dependent Claim
16 words
The biosensor of claim 1, wherein said electrode comprises an enzyme mediating oxidation of said analyte.
Claim 12
Dependent Claim
11 words
The biosensor of claim 10, wherein said enzyme is an oxidoreductase.
Claim 13
Dependent Claim
37 words
The biosensor of claim 11, wherein said oxidoreductase is glucose oxidase, lactate oxidase, xanthene oxidase, cholesterol oxidase, pyruvate oxidase, L-amino acid oxidase, D-amino acid oxidase, alcohol oxidase, urease, urate oxidase, aldehyde oxidase, glycollate oxidase, or sarcosine oxidase.
Claim 14
Dependent Claim
11 words
The biosensor of claim 12, wherein the oxidoreductase is glucose oxidase.
Claim 15
Dependent Claim
63 words
A process for analyzing an analyte in the presence of interferants in a sample comprising the steps of: contacting a sample with an interferant eliminating biosensor having an electrode and an interferant eliminating catalyst; substantially oxidizing interferants in said sample by an oxidation reaction catalyzed by the interferant eliminating catalyst; and detecting the analyte at the electrode in the absence of substantial interference.
Claim 16
Dependent Claim
63 words
A process for analyzing an analyte in the presence of interferants in a sample comprising the steps of: contacting a sample with an interferant eliminating biosensor having an electrode and an interferant eliminating catalyst; substantially oxidizing interferants in said sample by an oxidation reaction catalyzed by the interferant eliminating catalyst; and detecting the analyte at the electrode in the absence of substantial interference.
Claim 17
Dependent Claim
26 words
The process of claim 14, wherein said catalyst mediates the following reaction: ##STR5## where AH 2 represents an interfering compound and A represents a non-interfering compound.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-17 | 1998-04-18 | 1998-10-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-17 | 2002-04-18 | 2002-10-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-17 | 2006-04-18 | 2006-10-18 | $7700.00 | Window Closed / Lapsed |