US4390621A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and device for detecting glucose concentration
Assignee: Bayer Corp
•
Filing: 1982-03-01
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Granted: 1983-06-28
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Expires: 2002-03-01
Abstract
Abstract A method and test device for determining the glucose concentration in a test sample containing from 1/2 to 10 percent glucose are disclosed. The method and test device involve impregnating the carrier with an enzymatic testing composition which includes glucose oxidase, peroxidase and a chromogen. The carrier is subsequently impregnated with from 0.5 to 1.5 percent by weight polystyrene. Contact of the carrier with a glucose-containing test sample produces a detectable response whereby the glucose concentration can be determined.
Inventors
- 👤 Robert Bauer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 18:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; G01N33/523; C; C12; C12Q; C12Q1/00; C12Q1/54; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
96 words
In a method for determining the glucose concentration in a test sample containing from about 1/2 to 10 percent glucose which method includes the steps of; contacting said sample with a test device which includes a carrier matrix impregnated with an enzymatic testing composition including glucose oxidase, peroxidase, and a chromogen; and observing a detectable response produced by said test device; the improvement which comprises impregnating the carrier matrix with a solution of from about 0.5 percent to 1.5 percent by weight of polystyrene subsequent to the impregnating of the carrier matrix with said testing composition.
Claim 2
Dependent Claim
96 words
In a method for determining the glucose concentration in a test sample containing from about 1/2 to 10 percent glucose which method includes the steps of; contacting said sample with a test device which includes a carrier matrix impregnated with an enzymatic testing composition including glucose oxidase, peroxidase, and a chromogen; and observing a detectable response produced by said test device; the improvement which comprises impregnating the carrier matrix with a solution of from about 0.5 percent to 1.5 percent by weight of polystyrene subsequent to the impregnating of the carrier matrix with said testing composition.
Claim 3
Dependent Claim
12 words
A method as claimed in claim 1 wherein the carrier is paper.
Claim 4
Dependent Claim
20 words
A method as claimed in claim 1 wherein polystyrene is present in an amount of about 1.0 percent by weight.
Claim 5
Dependent Claim
19 words
A method as claimed in claim 1 wherein the chromogen is a benzidine-type indicator or a substituted aniline indicator.
Claim 6
Independent Claim
86 words
A method for determining the glucose concentration in a test sample containing from about 1/2 to 10 percent glucose which comprises the steps of providing a test device which includes a carrier having inpregnated therein an enzymatic testing composition comprising glucose oxidase, peroxidase, and a chromogen; subsequently impregnating the carrier with from 0.5 percent to 1.5 percent by weight of a solution of polystyrene; contacting said test sample with the carrier of said test device; and observing a detectable response, whereby the glucose concentration is determined.
Claim 7
Independent Claim
86 words
A method for determining the glucose concentration in a test sample containing from about 1/2 to 10 percent glucose which comprises the steps of providing a test device which includes a carrier having inpregnated therein an enzymatic testing composition comprising glucose oxidase, peroxidase, and a chromogen; subsequently impregnating the carrier with from 0.5 percent to 1.5 percent by weight of a solution of polystyrene; contacting said test sample with the carrier of said test device; and observing a detectable response, whereby the glucose concentration is determined.
Claim 8
Dependent Claim
12 words
A method as claimed in claim 5 wherein the carrier is paper.
Claim 9
Dependent Claim
21 words
A method as claimed in claim 5 wherein the polystyrene is present in an amount of about 1.0 percent by weight.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-27 | 1986-12-27 | 1987-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-27 | 1990-12-27 | 1991-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-27 | 1994-12-27 | 1995-06-28 | $7700.00 | Window Closed / Lapsed |