US4211845A
Expired / Lapsed
US
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Glucose indicator and method
Assignee: Bayer Corp
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Filing: 1978-11-24
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Granted: 1980-07-08
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Expires: 1998-11-24
Abstract
Abstract Glucose indicator, test means and method for assaying glucose in fluids are disclosed. More specifically, a new indicator formulation comprising an enzyme system which includes a substance having glucose oxidase activity, a substance having perioxidative activity and a mixture of syringaldazine and 3,3',5,5'-tetramethylbenzidine are disclosed for indicating the presence of glucose in industrial and body fluids. The indicator formulation is nonmutagenic and can be used in conjunction with instrumentation designed to quantitatively measure whole blood glucose levels within the range of 10 to 400 milligrams (mg) per 100 milliliters (ml).
Inventors
- 👤 Marvin A. Genshaw
- 👤 William I. White
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:39
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
36 words
A glucose indicator comprising glucose oxidase, a substance having peroxidase activity and a color-forming substance oxidizable by hydrogen peroxide in the presence of said peroxidase, said color-forming substance consisting of a mixture of 3,3',5,5',-tetramethylbenzidine and syringaldazine.
Claim 2
Dependent Claim
36 words
A glucose indicator comprising glucose oxidase, a substance having peroxidase activity and a color-forming substance oxidizable by hydrogen peroxide in the presence of said peroxidase, said color-forming substance consisting of a mixture of 3,3',5,5',-tetramethylbenzidine and syringaldazine.
Claim 3
Dependent Claim
26 words
The glucose indicator of claim 1 in which the 3,3',5,5'-tetramethylbenzidine and syringaldazine are present in a molar ratio ranging from about 1:8 to about 8:1, respectively.
Claim 4
Dependent Claim
26 words
The glucose indicator of claim 1 in which the 3,3'5,5'-tetramethylbenzidine and syringaldazine are present in a molar ratio of from about 2:6 to about 1:1, respectively.
Claim 5
Dependent Claim
21 words
The glucose indicator of claim 1 in which the 3,3',5,5'-tetramethylbenzidine and syringaldazine are present in a molar ratio of 3:5, respectively.
Claim 6
Dependent Claim
36 words
Method for determining the presence of glucose in a fluid sample, which method comprises bringing a sample to be tested into contact with the glucose indicator as defined in claim 1 and observing any color change.
Claim 7
Dependent Claim
36 words
Method for determining the presence of glucose in a fluid sample, which method comprises bringing a sample to be tested into contact with the glucose indicator as defined in claim 2 and observing any color change.
Claim 8
Dependent Claim
36 words
Method for determining the presence of glucose in a fluid sample, which method comprises bringing a sample to be tested into contact with the glucose indicator as defined in claim 3 and observing any color change.
Claim 9
Dependent Claim
36 words
Method for determining the presence of glucose in a fluid sample, which method comprises bringing a sample to be tested into contact with the glucose indicator as defined in claim 4 and observing any color change.
Claim 10
Dependent Claim
14 words
The test means comprising a carrier matrix incorporating the glucose indicator of claim 1.
Claim 11
Dependent Claim
13 words
Test means comprising a carrier matrix incorporating the glucose indicator of claim 2.
Claim 12
Dependent Claim
110 words
Test means for detecting glucose in a fluid which comprises a bibulous material incorporating a mixture which consists essentially of an enzyme system which includes a substance having glucose oxidase activity, a substance having peroxidative activity, polyvinyl pyrrolidone, a derivative of an interpolymer of methylvinyl ether and maleic anhydride, a buffer system consisting essentially of a tris (Hydroxymethyl)-amine methane, malonic acid and disodium malonate and an indicator mixture which is oxidized in the presence of a peroxide and the substance having peroxidative activity and which changes color thereon, said indicator mixture consisting essentially of a mixture of 3,3',5,5'-tetramethylbenzidine and syringaldazine in a molar ratio from about 1:8 to 8:1, respectively.
Claim 13
Dependent Claim
110 words
Test means for detecting glucose in a fluid which comprises a bibulous material incorporating a mixture which consists essentially of an enzyme system which includes a substance having glucose oxidase activity, a substance having peroxidative activity, polyvinyl pyrrolidone, a derivative of an interpolymer of methylvinyl ether and maleic anhydride, a buffer system consisting essentially of a tris (Hydroxymethyl)-amine methane, malonic acid and disodium malonate and an indicator mixture which is oxidized in the presence of a peroxide and the substance having peroxidative activity and which changes color thereon, said indicator mixture consisting essentially of a mixture of 3,3',5,5'-tetramethylbenzidine and syringaldazine in a molar ratio from about 1:8 to 8:1, respectively.
Claim 14
Dependent Claim
27 words
Test means as described in claim 11 in which the test means also has a semi-permeable coating of a transparent ethylcellulose film over the impregnated bibulous material.
Claim 15
Dependent Claim
31 words
A method for determining the presence of glucose in a fluid sample comprising contacting a sample to be tested with the test means of claim 9 and observing any color change.
Claim 16
Dependent Claim
31 words
A method for determining the presence of glucose in a fluid sample comprising contacting a sample to be tested with the test means of claim 10 and observing any color change.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-07-08 | 1984-01-07 | 1984-07-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-07-08 | 1988-01-07 | 1988-07-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-07-08 | 1992-01-07 | 1992-07-08 | $7700.00 | Window Closed / Lapsed |