US5795453A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrodes and metallo isoindole ringed compounds
Assignee: Individual
•
Filing: 1996-01-23
•
Granted: 1998-08-18
•
Expires: 2016-01-23
Abstract
Abstract The present invention provides for electrode assemblies and methods in which electron transfer between a redox reaction product and an electrically conductive electrode material is facilitated by a metallo macrocyclic compound, preferably a metallo isoindole ringed compound and more preferably a ferro isoindole ringed compound. The redox reaction is usually catalysized by a redox enzyme, such as an oxidase.
Inventors
- 👤 Markas A. T. Gilmartin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 02:34
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; G; G01; G01N; G01N31/00; G01N31/22; G01N31/228; A; A61; A61B; A61B5/00; A61B5/40; A61B5/4058; A61B5/4064; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
60 words
An hydrogen peroxide electrode assembly comprising: a) a ferro isoindole ringed compound, b) an electrode, and c) an oxidase, wherein said oxidase is attached to an analyte or an analyte analog and said oxidase can prevent said analyte from binding to an analyte binding moiety; and wherein said oxidase has sufficient activity to catalyse hydrogen peroxide production in detectable amounts.
Claim 2
Dependent Claim
60 words
An hydrogen peroxide electrode assembly comprising: a) a ferro isoindole ringed compound, b) an electrode, and c) an oxidase, wherein said oxidase is attached to an analyte or an analyte analog and said oxidase can prevent said analyte from binding to an analyte binding moiety; and wherein said oxidase has sufficient activity to catalyse hydrogen peroxide production in detectable amounts.
Claim 3
Dependent Claim
61 words
A The hydrogen peroxide electrode of claim 1, wherein said electrode comprises electrically conductive carbon and said ferro isoindole ringed compound is C 32 H 18 N 8 Fe(II) and said C 32 H 18 N 8 Fe(II) is in an amount to provide for sufficient electron transfer to permit detection of an electrical current related to catalysis by said oxidase.
Claim 4
Dependent Claim
20 words
The hydrogen peroxide electrode of claim 2, wherein said ferro isoindole ringed compound is dispersed in said electrically conductive carbon.
Claim 5
Dependent Claim
24 words
The hydrogen peroxide electrode of claim 3, wherein said ferro isoindole ringed compound mass is at least 1% of said electrically conductive carbon mass.
Claim 6
Dependent Claim
25 words
The hydrogen peroxide electrode of claim 4, wherein said ferro isoindole ringed compound mass is between 3 and 8% of said electrically conductive carbon mass.
Claim 7
Dependent Claim
51 words
The hydrogen peroxide electrode of claim 3, further comprising a selectively permeable layer either 1) between said oxidase and said hydrogen peroxide electrode or 2) covering both said oxidase and said hydrogen peroxide electrode, wherein said selectively permeable layer excludes medium interferents of molecular weight 700-2500 from said hydrogen peroxide electrode.
Claim 8
Dependent Claim
14 words
The hydrogen peroxide electrode assembly of claim 6, wherein said electrode is screen printed.
Claim 9
Dependent Claim
21 words
The hydrogen peroxide electrode assembly of claim 7, wherein said hydrogen peroxide electrode has a thickness between 1,000 and 50 μ.
Claim 10
Dependent Claim
44 words
The hydrogen peroxide electrode assembly of claim 8, wherein said analyte binding moiety is attached to a matrix and said matrix electrically communicates with said hydrogen peroxide electrode in the presence of a liquid containing a sufficient amount electrolytes to provide for electrical communication.
Claim 11
Dependent Claim
27 words
The hydrogen peroxide electrode assembly of claim 9, wherein said matrix is part of a fluid guidance pathway that is in fluid communication with a waste reservoir.
Claim 12
Dependent Claim
23 words
The hydrogen peroxide electrode assembly of claim 8, wherein said ferro isoindole ringed compound is also present in a layer covering said electrode.
Claim 13
Dependent Claim
16 words
The hydrogen peroxide electrode of claim 11, wherein said oxidase is dry and covers said electrode.
Claim 14
Dependent Claim
22 words
The hydrogen peroxide electrode assembly of claim 12, wherein said ferro isoindole ringed compound is not dispersed in said electrically conductive carbon.
Claim 15
Dependent Claim
27 words
The hydrogen peroxide electrode assembly of claim 13, further comprising a matrix, wherein said matrix permits fluid communication between said oxidase and said ferro isoindole ringed compound.
Claim 16
Dependent Claim
50 words
The hydrogen peroxide electrode assembly of claim 14, wherein said ferro isoindole ringed compound is present on a surface of said hydrogen peroxide electrode or said ferro isoindole ringed compound is present in said matrix and said matrix permits electrical communication between said ferro isoindole ringed compound and said electrode.
Claim 17
Dependent Claim
17 words
The hydrogen peroxide electrode assembly of claims 15, wherein said oxidase is covalently attached to said matrix.
Claim 18
Dependent Claim
17 words
The hydrogen peroxide electrode assembly of claim 15, wherein said oxidase is noncovalently attached to said matrix.
Claim 19
Dependent Claim
22 words
The hydrogen peroxide electrode assembly of claim 17, wherein said oxidase is attached to said matrix through a first analyte binding moiety.
Claim 20
Dependent Claim
34 words
The hydrogen peroxide electrode assembly of claim 17, wherein said oxidase is attached to said matrix through a second analyte binding moiety, wherein said first analyte binding moiety is covalently attached to said matrix.
Claim 21
Dependent Claim
45 words
The hydrogen peroxide electrode assembly of claim 18, wherein said oxidase is attached to a second analyte binding moiety and said second analyte binding moiety is attached to said matrix through an analyte that is attached to said matrix through said first analyte binding moiety.
Claim 22
Dependent Claim
20 words
The hydrogen peroxide electrode assembly of claim 20, wherein said ferro isoindole ringed compound is a ferrous isoindole ringed compound.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2001-08-17 | 2002-02-16 | 2002-08-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-08-17 | 2006-02-16 | 2006-08-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-08-17 | 2010-02-16 | 2010-08-18 | $7700.00 | Window Closed / Lapsed |