EP0251915A2
Expired / Lapsed
EP
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Enzyme sensor
Assignee: Terumo Corp
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Filing: 1987-06-26
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Granted: —
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Expires: 2007-06-26
Abstract
Abstract Disclosed is an enzyme sensor for measuring a predetermined substrate concentration by potentiometric or amperometric response on the basis of an enzyme. In one aspect, a solid-state enzyme sensor measures a predetermined substrate concentration by potentiometric response and includes an electrically conductive base (1), redox layer or ion-sensitive layer coated with a solid enzyme layer (4). In another aspect, a solid-state enzyme sensor measures a predetermined substrate concentration by amperometric response and includes a solid enzyme membrane-coated oxygen reduction catalyst layer. Also disclosed is an enzyme control layer for controlling the outflow and inflow of the enzyme layer.
Inventors
- 👤 Shuichiro Yamaguchi
- 👤 Naoto Uchida
- 👤 Takeshi Shimomura
- 👤 Noboru Oyama
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 18:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/58; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
66 words
An enzyme sensor for measuring a substrate concentration on the basis of an enzyme, comprising : an electrically conductive base (1) ; and an enzyme Layer (4) consisting of an enzyme and coating a surface of said electrically conductive base (1) for promoting a chemical reaction of said substrate ; the concentration of said substrate being measured by an electric potential of said electrically conductive base.
Claim 2
Dependent Claim
66 words
An enzyme sensor for measuring a substrate concentration on the basis of an enzyme, comprising : an electrically conductive base (1) ; and an enzyme Layer (4) consisting of an enzyme and coating a surface of said electrically conductive base (1) for promoting a chemical reaction of said substrate ; the concentration of said substrate being measured by an electric potential of said electrically conductive base.
Claim 3
Dependent Claim
34 words
The enzyme sensor according to claim 1, wherein said enzyme is glucose oxidase for measuring glucose concentration, urease for measuring urea concentration, uricase for measuring uric acid concentration, cholesterol oxidase for measuring cholesterol concentration.
Claim 4
Dependent Claim
20 words
The enzyme sensor according to claim 1, wherein said enzyme is imparted with a cross-linked structure by a cross-linking agent.
Claim 5
Dependent Claim
19 words
The enzyme sensor according to claim 1, wherein said electrically conductive base (1) is carbon having a graphite structure.
Claim 6
Dependent Claim
76 words
An enzyme sensor for measuring a substrate concentration on the basis of an enzyme, comprising : an electrically conductive base (1); a redox Layer (5) coating a surface of said electrically conductive base ; and an enzyme Layer (4) consisting of an enzyme and coating a surface of said redox layer for promoting a chemical reaction of said substrate ; the concentration of said substrate being measured by an electric potential of said electrically conductive base.
Claim 7
Dependent Claim
76 words
An enzyme sensor for measuring a substrate concentration on the basis of an enzyme, comprising : an electrically conductive base (1); a redox Layer (5) coating a surface of said electrically conductive base ; and an enzyme Layer (4) consisting of an enzyme and coating a surface of said redox layer for promoting a chemical reaction of said substrate ; the concentration of said substrate being measured by an electric potential of said electrically conductive base.
Claim 8
Dependent Claim
34 words
The enzyme sensor according to claim 5, wherein said enzyme is glucose oxidase for measuring glucose concentration, urease for measuring urea concentration, uricase for measuring uric acid concentration, cholesterol oxidase for measuring cholesterol concentration.
Claim 9
Dependent Claim
20 words
The enzyme sensor according to claim 5, wherein said enzyme is imparted with a cross-linked structure by a cross-linking agent.
Claim 10
Dependent Claim
18 words
The enzyme sensor according to claim 5, wherein said electrically conductive base is carbon having a graphite structure.
Claim 11
Dependent Claim
35 words
The enzyme sensor according to claim 5, wherein said redox layer is selected from a group of substances having a quinone hydroxynone-type oxidation-reduction function, an amino quinoid-type oxidation-reduction function, or a poLy(pyrroLe), poLy(cheniLene) oxidation-reduction function.
Claim 12
Dependent Claim
100 words
An enzyme sensor for measuring a substrate concentration on the basis of a moLecuLar identifying function of an enzyme, comprising : an electrically conductive base (1) ; a redox Layer (5) coating a surface of said eLectricaLLy conductive base ; an ion-sensitive Layer (6) coating a surface of said redox layer for responding to a predetermined ion ; and an enzyme Layer (4) consisting of an enzyme and coating a surface of said ion-sensitive Layer for promoting a chemical reaction of said substrate ; the concentration of said substrate being measured by an electric potential of said eLectricaLLy conductive base.
Claim 13
Dependent Claim
100 words
An enzyme sensor for measuring a substrate concentration on the basis of a moLecuLar identifying function of an enzyme, comprising : an electrically conductive base (1) ; a redox Layer (5) coating a surface of said eLectricaLLy conductive base ; an ion-sensitive Layer (6) coating a surface of said redox layer for responding to a predetermined ion ; and an enzyme Layer (4) consisting of an enzyme and coating a surface of said ion-sensitive Layer for promoting a chemical reaction of said substrate ; the concentration of said substrate being measured by an electric potential of said eLectricaLLy conductive base.
Claim 14
Dependent Claim
34 words
The enzyme sensor according to claim 10, wherein said enzyme is glucose oxidase for measuring glucose concentration, urease for measuring urea concentration, uricase for measuring uric acid concentration, cholesterol oxidase for measuring cholesterol concentration.
Claim 15
Dependent Claim
20 words
The enzyme sensor according to claim 10, wherein said enzyme is imparted with a cross-linked structure by a cross-linking agent.
Claim 16
Dependent Claim
18 words
The enzyme sensor according to claim 10, wherein said electrically conductive base is carbon having a graphite structure.
Claim 17
Dependent Claim
35 words
The enzyme sensor according to claim 10, wherein said redox layer is selected from a group of substances having a quinone hydroxynone-type oxidation-reduction function, an amino quinoid-type oxidation-reduction function or, a poly(pyrrole), poly(chenilene) oxidation-reduction function.
Claim 18
Dependent Claim
72 words
An enzyme sensor for measuring a substrate concentration on the basis of a molecular identifying function of an enzyme, comprising : an electrically conductive base (11) ; an oxygen reduction catalyst Layer (16) coating said electrically conductive base ; and an enzyme Layer containing (17) an enzyme and coating said oxygen reduction catalyst Layer ; wherein the concentration of an oxygen consumed by an enzyme reaction is measured by a redox current.
Claim 19
Dependent Claim
72 words
An enzyme sensor for measuring a substrate concentration on the basis of a molecular identifying function of an enzyme, comprising : an electrically conductive base (11) ; an oxygen reduction catalyst Layer (16) coating said electrically conductive base ; and an enzyme Layer containing (17) an enzyme and coating said oxygen reduction catalyst Layer ; wherein the concentration of an oxygen consumed by an enzyme reaction is measured by a redox current.
Claim 20
Dependent Claim
28 words
The enzyme sensor according to claim 15, wherein said oxygen reduction catalyst Layer is selected from polymer membranes such as a cobalt phthalocyanine complex and cobalt porphyrin complex.
Claim 21
Dependent Claim
24 words
The enzyme sensor according to claim 15, wherein the enzyme layer comprises the enzyme fixed to the oxygen reduction catalyst Layer by electrolytic polymerization.
Claim 22
Dependent Claim
35 words
The enzyme sensor according to claim 17, wherein said enzyme is glucose oxidase for measuring glucose concentration, urease for measuring urea concentration, uricase for measuring uric acid concentration or, cholesterol oxidase for measuring cholesterol concentration.
Claim 23
Dependent Claim
20 words
The enzyme sensor according to claim 17, wherein said enzyme is imparted with a cross-linked structure by a cross-linking agent.
Claim 24
Dependent Claim
25 words
The enzyme sensor according to claim 15, wherein said electrically conductive base is selected from the group consisting of carbon fiber, graphite, platinum and gold.
Claim 25
Dependent Claim
47 words
An enzyme sensor for measuring a substrate concentration on the basis of a molecular identifying function of an enzyme, comprising : an enzyme Layer containing an enzyme, and an enzyme control layer coating said enzyme Layer for controlling outflow of the enzyme and inflow of the substrate.
Claim 26
Dependent Claim
47 words
An enzyme sensor for measuring a substrate concentration on the basis of a molecular identifying function of an enzyme, comprising : an enzyme Layer containing an enzyme, and an enzyme control layer coating said enzyme Layer for controlling outflow of the enzyme and inflow of the substrate.
Claim 27
Dependent Claim
24 words
The enzyme sensor according to claim 21, wherein the enzyme Layer comprises the enzyme fixed to an oxygen reduction catalyst layer by eLectroLytic polymerization.
Claim 28
Dependent Claim
35 words
The enzyme sensor according to claim 22, wherein said enzyme is glucose oxidase for measuring glucose concentration, urease for measuring urea concentration, uricase for measuring uric acid concentration, or cholesterol oxidase for measuring cholesterol concentration.
Claim 29
Dependent Claim
20 words
The enzyme sensor according to claim 22, wherein said enzyme is imparted with a cross-linked structure by a cross-linking agent.
References Cited (Backward Citations)
No citation records indexed.
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