Enzyme sensor and method of manufacturing the same

Abstract

Abstract An enzyme sensor is disclosed, which has a two-layer fixed film structure consisting of a reduction function layer including an electron movement medium and di-valent iron ions and an enzyme-fixed layer covering said reduction function layer, and in which hydrogen peroxide generated through decomposition of glucose or the like in the enzyme-fixed layer is reduced by electron movement medium and di-valent iron ions in the reduction function layer. Thus, the construction of enzyme sensor is simplified, measurement is extremely facilitated, and the measurement time is reduced. As a different principle of enzyme sensor, an electrically conductive substrate of conductive carbon or the like is covered with an oxygen-reducing catalytic function layer and enzyme layer. Thus, there is no problem of contamination of enzyme sensor, and it is possible to readily manufacture and miniaturize enzyme sensors.

Inventors

• 👤 Noboru Oyama
• 👤 Takeshi Shimomura
• 👤 Shuichiro Yamaguchi

Bibliographic Details