Abstract An enzyme sensor is disclosed, which has a two-layer fixed film structure consisting of a reduction function layer including an electron movement medium and di-valent iron ions and an enzyme-fixed layer covering said reduction function layer, and in which hydrogen peroxide generated through decomposition of glucose or the like in the enzyme-fixed layer is reduced by electron movement medium and di-valent iron ions in the reduction function layer. Thus, the construction of enzyme sensor is simplified, measurement is extremely facilitated, and the measurement time is reduced. As a different principle of enzyme sensor, an electrically conductive substrate of conductive carbon or the like is covered with an oxygen-reducing catalytic function layer and enzyme layer. Thus, there is no problem of contamination of enzyme sensor, and it is possible to readily manufacture and miniaturize enzyme sensors.
Inventors
👤 Noboru Oyama
👤 Takeshi Shimomura
👤 Shuichiro Yamaguchi
Bibliographic Details
Application Number—
JurisdictionUS
Data Sourcegoogle_patents
Last Synchronized2026-10-28 21:19
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
80 words
An enzyme sensor comprising: an electrically conductive substrate; a reduction function layer covering at least part of said electrically conductive substrate, including an electron movement medium with a structure represented as [Ru(NH 3 ) 6 ] 3+ or [Ru(NH 3 ) 5 X], where X represents pyridine, halogen ion, nicotinamide or crystal water, and di-valent iron ions, and capable of causing a reduction reaction of hydrogen peroxide; and an enzyme-fixed layer covering at least part of said reduction function layer.
Claim 2
Dependent Claim
80 words
An enzyme sensor comprising: an electrically conductive substrate; a reduction function layer covering at least part of said electrically conductive substrate, including an electron movement medium with a structure represented as [Ru(NH 3 ) 6 ] 3+ or [Ru(NH 3 ) 5 X], where X represents pyridine, halogen ion, nicotinamide or crystal water, and di-valent iron ions, and capable of causing a reduction reaction of hydrogen peroxide; and an enzyme-fixed layer covering at least part of said reduction function layer.
Claim 3
Dependent Claim
50 words
An enzyme sensor comprising: an electrically conductive substrate comprising a carbon fiber with a sectional area of 7.85×10 -7 cm 2 , an oxygen electrode layer covering said electrically conductive substrate and including a coordinator compound having an oxygen-reducing catalytic function; and an enzyme-fixed layer covering said oxygen electrode layer.
Claim 4
Dependent Claim
50 words
An enzyme sensor comprising: an electrically conductive substrate comprising a carbon fiber with a sectional area of 7.85×10 -7 cm 2 , an oxygen electrode layer covering said electrically conductive substrate and including a coordinator compound having an oxygen-reducing catalytic function; and an enzyme-fixed layer covering said oxygen electrode layer.
Claim 5
Dependent Claim
17 words
The enzyme sensor according to claim 2, wherein said coordinator compound consists of a cyclic nitrogen-containing compound.
Claim 6
Dependent Claim
32 words
The enzyme sensor according to claim 3, wherein said coordinator compound is at least one member selected from the group consisting of a porphyrin derivative, a phthalocyanine derivative and a phenanthroline derivative.