US4882013A
Expired / Lapsed
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Application of tetrathiafulvalenes in bioelectrochemical processes
Assignee: Cranfield Biotechnology Ltd
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Filing: 1987-02-24
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Granted: 1989-11-21
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Expires: 2007-02-24
Abstract
Abstract Bioelectrochemical processes use tetrathiafulvalenes (TTF) as a mediator of electron transfer between biological systems and electrodes. Typically it finds use in bioelectrochemical assays, e.g. involving glucose oxidase mediated oxidation of glucose. The TTF may be immobilized on the electrode surface, as may one or more enzymes involved in the process.
Inventors
- 👤 Anthony P. F. Turner
- 👤 Stuart P. Hendry
- 👤 Marco F. Cardosi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 13:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
65 words
A bioelectrochemical system comprising: a biological system comprising a redox system which requires electron transfer for its operation, wherein said redox system comprises an enzyme; an electrode; and a mediator for transferring electrons between said electrode and the enzyme of said redox system for operation of said redox system, characterized in that said mediator is tetrathiafulvalene, and is not complexed with an electron acceptor molecule.
Claim 2
Dependent Claim
65 words
A bioelectrochemical system comprising: a biological system comprising a redox system which requires electron transfer for its operation, wherein said redox system comprises an enzyme; an electrode; and a mediator for transferring electrons between said electrode and the enzyme of said redox system for operation of said redox system, characterized in that said mediator is tetrathiafulvalene, and is not complexed with an electron acceptor molecule.
Claim 3
Dependent Claim
12 words
The bioelectrochemical system of claim 1 wherein the tetrathiafulvalene is in solution.
Claim 4
Dependent Claim
14 words
The bioelectrochemical system of claim 1 wherein the tetrathiafulvalene is deposited on said electrode.
Claim 5
Dependent Claim
19 words
The bioelectrochemical system of claim 1 wherein said enzyme is an oxidoreductase enzyme and is immobilized on said electrode.
Claim 6
Dependent Claim
20 words
The bioelectrochemical system of claim 4 wherein the enzyme is a glycoenzyme immobilized on the electrode via its carbohydrate groups.
Claim 7
Dependent Claim
28 words
The bioelectrochemical system of claim 1 wherein the redox system effects the oxidation of glucose catalyzed by said enzyme which is selected from glucose oxidase and glucose dehydrogenase.
Claim 8
Dependent Claim
23 words
The bioelectrochemical system of claim 1 wherein said redox system is selected from enzymes, cell fragments, intact cells, tissues, and enzyme-labeled affinity reactions.
Claim 9
Dependent Claim
28 words
The bioelectrochemical system of claim 1 wherein said redox system effects the oxidation of an amino acid by said enzyme, and wherein said enzyme is amino acid oxidase.
Claim 10
Dependent Claim
84 words
An assay procedure for assaying a substance, comprising: causing the substance to participate in a redox system provided by a biological system, said redox system requiring electron transfer for its operation, wherein said redox system comprises an enzyme; providing an electrode; employing said electrode in an electrochemical cell including said redox system; providing tetrathiafulvalene as a mediator for transferring electrons between said electrode and the enzyme of said redox system for operation of said redox system; and deriving assay data from the electrode's response.
Claim 11
Dependent Claim
84 words
An assay procedure for assaying a substance, comprising: causing the substance to participate in a redox system provided by a biological system, said redox system requiring electron transfer for its operation, wherein said redox system comprises an enzyme; providing an electrode; employing said electrode in an electrochemical cell including said redox system; providing tetrathiafulvalene as a mediator for transferring electrons between said electrode and the enzyme of said redox system for operation of said redox system; and deriving assay data from the electrode's response.
Claim 12
Dependent Claim
26 words
A procedure according to claim 9 wherein said biological system comprises a redox system selected from enzymes, cell fragments, intact cells, tissues, and enzyme-labeled affinity reactions.
Claim 13
Dependent Claim
36 words
An electrode for use in a bioelectrochemical system, said electrode consisting essentially of a conductive surface onto which a tetrathiafulvalene and an oxidoreductase enzyme are deposited, wherein said tetrathiafulvalene is not complexed with an electron acceptor.
Claim 14
Dependent Claim
36 words
An electrode for use in a bioelectrochemical system, said electrode consisting essentially of a conductive surface onto which a tetrathiafulvalene and an oxidoreductase enzyme are deposited, wherein said tetrathiafulvalene is not complexed with an electron acceptor.
Claim 15
Dependent Claim
23 words
An electrode according to claim 11 wherein the enzyme is a glycoenzyme and is immobilised on the conductive surface via its carbohydrate groups.
Claim 16
Dependent Claim
21 words
An electrode according to claim 11 wherein said oxidoreductase enzyme is selected from glucose oxidase, glucose dehydrogenase, and amino acid oxidase.
Claim 17
Dependent Claim
35 words
An electrode for use in a bioelectrochemical system, said electrode comprising a graphite conductive surface onto which a coating consisting essentially of tetrathiafulvalene is provided, wherein said tetrathiafulvalene is not complexed with an electron acceptor.
Claim 18
Dependent Claim
35 words
An electrode for use in a bioelectrochemical system, said electrode comprising a graphite conductive surface onto which a coating consisting essentially of tetrathiafulvalene is provided, wherein said tetrathiafulvalene is not complexed with an electron acceptor.
References Cited (Backward Citations)
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