Patent US5328821A successfully refreshed from Google Patents.
US5328821A
Expired / Lapsed
US
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Cold and cryo-preservation methods for human tissue slices
Assignee: Individual
•
Filing: 1991-12-12
•
Granted: 1994-07-12
•
Expires: 2011-12-12
Abstract
Abstract Cold-and cryo-preservation solutions for tissue slices include a limited amount of glucose. The solutions provide extended cold storage of the slices without a loss of viability. The solutions also allow cryopreservation of the slices, so that the slices may be stored until needed and then thawed, cultured, packaged and distributed.
Inventors
- 👤 Robyn Fisher
- 👤 Klaus Brendel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 14:36
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A01N1/125
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
71 words
A method for the cold preservation of organ tissue comprising storing said tissue in an aqueous preservation solution comprising: 1-5 mM glucose; 30-120 mM gluconate; 30-120 mM saccharate; 25-30 mM potassium phosphate(monobasic); 1-3 mM sodium succinate; 3-6 mM magnesium sulfate; 1-3 mM magnesium chloride; 1-3 mM potassium bicarbonate; 2-4 mM fructose; 3-6 mM glutathione; 2-10 mM ascorbic acid; 3-5 mM adenosine and micromolar amounts of deferoxamine mesylate and gentamicin or penicillin.
Claim 2
Independent Claim
71 words
A method for the cold preservation of organ tissue comprising storing said tissue in an aqueous preservation solution comprising: 1-5 mM glucose; 30-120 mM gluconate; 30-120 mM saccharate; 25-30 mM potassium phosphate(monobasic); 1-3 mM sodium succinate; 3-6 mM magnesium sulfate; 1-3 mM magnesium chloride; 1-3 mM potassium bicarbonate; 2-4 mM fructose; 3-6 mM glutathione; 2-10 mM ascorbic acid; 3-5 mM adenosine and micromolar amounts of deferoxamine mesylate and gentamicin or penicillin.
Claim 3
Independent Claim
24 words
The method of 1 wherein the solution further comprises a cryoprotectant and the method further comprises immersing the preserved tissue into a crygenic fluid.
Claim 4
Independent Claim
24 words
The method of 1 wherein the solution further comprises a cryoprotectant and the method further comprises immersing the preserved tissue into a crygenic fluid.
Claim 5
Dependent Claim
22 words
The method of claim 2 wherein said cryoprotectant is selected from the group consisting of dimethyl sulfoxide, glycerol, ethylene glycol and propanediol.
Claim 6
Dependent Claim
26 words
The method of claim 2 further comprising the step of cooling the tissue at a controlled rate prior to immersing the tissue in the cryogenic fluid.
Claim 7
Dependent Claim
20 words
The method of claim 2 wherein said cryoprotectant comprises from about 5% to about 50% by weight of said solution.
Claim 8
Dependent Claim
34 words
The method of claim 2 wherein said storing step comprises sequentially placing the tissue in a series of solutions, each solution in the sequence having a greater concentration of cryoprotectant than the previous solution.
Claim 9
Dependent Claim
16 words
The method of claim 2 further comprising the step of thawing the cryopreserved human tissue slices.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-07-11 | 1998-01-10 | 1998-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-07-11 | 2002-01-10 | 2002-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-07-11 | 2006-01-10 | 2006-07-12 | $7700.00 | Window Closed / Lapsed |