Patent US307555A successfully refreshed from Google Patents.
US4565643A
Expired / Lapsed
US
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Antifreezing agent
Assignee: Kanegafuchi Chemical Industry Co Ltd
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Filing: 1984-09-07
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Granted: 1986-01-21
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Expires: 2004-09-07
Abstract
Abstract An antifreezing agent used in an aqueous medium system comprising a protein which is enzymatically modified with covalent incorporation of an amino acid ester. The antifreezing agent can keep an unfrozen state of the system down to -10° C., and does not exert any harmful effect to biological bodies.
Inventors
- 👤 Soichi Arai
- 👤 Michiko Watanabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 02:11
CPC Classifications
C; C09; C09K; C09K5/00; C09K5/20
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
57 words
A method for preventing destruction in biological tissues caused from the formation of ice crystals during exposure to a low temperature or a frozen state, said method comprising; contacting the biological tissues with an anti-freezing agent comprising, in an aqueous medium system, a protein which is enzymatically modified with the covalent incorporation of an amino acid ester.
Claim 2
Independent Claim
57 words
A method for preventing destruction in biological tissues caused from the formation of ice crystals during exposure to a low temperature or a frozen state, said method comprising; contacting the biological tissues with an anti-freezing agent comprising, in an aqueous medium system, a protein which is enzymatically modified with the covalent incorporation of an amino acid ester.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein the amino acid is leucine, alanine or norleucine.
Claim 4
Dependent Claim
30 words
The method of claim 1, wherein the amino acid ester is composed of an alcoholic portion which is an aliphatic alcohol having 4 to 16 carbon atoms in its molecule.
Claim 5
Dependent Claim
18 words
The method of claim 1, wherein the proteih is casein, gelatin, albumin, lactoalbumin, soy protein or fish protein.
Claim 6
Dependent Claim
12 words
The method of claim 1, wherein the enzyme is a thiol protease.
Claim 7
Dependent Claim
18 words
The method of claim 1, wherein the molecular weight of the enzymatically modified protein is 2,000 to 40,000.
Claim 8
Dependent Claim
27 words
The method of claim 1, wherein the enzymatically modified protein is a product prepared by covalently attaching leucine dodecyl ester to gelatin by the action of papain.
Claim 9
Dependent Claim
17 words
The method of claim 1, wherein the aqueous medium is an aqueous medium contained in biological tissues.
Claim 10
Dependent Claim
17 words
The method of claim 1, wherein the aqueous medium system is a water-in-oil or oil-in-water emulsion system.
Claim 11
Dependent Claim
30 words
The method of claim 2, wherein the amino acid ester is composed of an alcoholic portion which is an aliphatic alcohol having 4 to 16 carbon atoms in its molecule.
References Cited (Backward Citations)
US3712865A
Cited
GB2001095A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-01-20 | 1989-07-22 | 1990-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-20 | 1993-07-22 | 1994-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-01-20 | 1997-07-22 | 1998-01-21 | $7700.00 | Window Closed / Lapsed |