US4920044A
Expired / Lapsed
US
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Intracellular flush solution for preserving organs
Assignee: Cleveland Clinic Foundation
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Filing: 1988-11-08
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Granted: 1990-04-24
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Expires: 2008-11-08
Abstract
Abstract The present invention is directed to a new hypersomotic intracellular flush and storage solution for preserving an organ for transplantation. In addition, the present invention is directed to a method for preserving an organ to be transplanted by using the flush solution of the invention. Among the components of the solution are various physiologic salts, mannitol, adenosine, allopurinol and verapamil.
Inventors
- 👤 Peter D. Bretan, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:43
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
147 words
An intracellular flush solution for preserving an organ for transplantation, comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 0 and 4.62 grams per liter of MgSO 4 ; (h) between about 0 and 0.4 meq/ml of MgCl 2 ; (i) between about 0.75 and 1.25 grams per liter of adenosine; (j) between about 0 and 0.125 grams per liter of allopurinol; and, (k) between about 0 and 1.0 grams per liter of verapamil.
Claim 2
Dependent Claim
147 words
An intracellular flush solution for preserving an organ for transplantation, comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 0 and 4.62 grams per liter of MgSO 4 ; (h) between about 0 and 0.4 meq/ml of MgCl 2 ; (i) between about 0.75 and 1.25 grams per liter of adenosine; (j) between about 0 and 0.125 grams per liter of allopurinol; and, (k) between about 0 and 1.0 grams per liter of verapamil.
Claim 3
Dependent Claim
32 words
The intracellular flush solution of claim 1, wherein said mannitol is present in an amount sufficient to adjust the osmolality of the solution to a value between about 255 and 425 mOsm/Kg.
Claim 4
Dependent Claim
134 words
An intracellular flush solution for preserving an organ for transplantation, comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 0.2 and 0.4 meq/ml of MgCl 2 ; (h) between about 0.75 and 1.25 grams per liter of adenosine; (i) between about 0 and 0.125 grams per liter of allopurinol; and, (j) between about 0 and 1.0 grams per liter of verapamil.
Claim 5
Dependent Claim
134 words
An intracellular flush solution for preserving an organ for transplantation, comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 0.2 and 0.4 meq/ml of MgCl 2 ; (h) between about 0.75 and 1.25 grams per liter of adenosine; (i) between about 0 and 0.125 grams per liter of allopurinol; and, (j) between about 0 and 1.0 grams per liter of verapamil.
Claim 6
Dependent Claim
66 words
An intracellular flush solution for preserving an organ for transplantation comprising: about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 7
Dependent Claim
66 words
An intracellular flush solution for preserving an organ for transplantation comprising: about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 8
Dependent Claim
83 words
An intracellular flush solution for preserving an organ for transplantation comprising: about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 2.3 grams per liter KHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 0.1 grams per liter allopurinol; and, about 0.007 verapamil.
Claim 9
Dependent Claim
83 words
An intracellular flush solution for preserving an organ for transplantation comprising: about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 2.3 grams per liter KHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 0.1 grams per liter allopurinol; and, about 0.007 verapamil.
Claim 10
Dependent Claim
78 words
An intracellular flush solution for preserving an organ for transplantation comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; and, (d) about 1.0 grams per liter adenosine.
Claim 11
Dependent Claim
78 words
An intracellular flush solution for preserving an organ for transplantation comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; and, (d) about 1.0 grams per liter adenosine.
Claim 12
Dependent Claim
101 words
An intracellular flush solution for preserving an organ for transplantation comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; (d) about 1.0 grams per liter adenosine; (e) about 2.3 grams per liter KHCO 3 ; (f) about 0.1 grams per liter allopurinol; and, (g) about 0.007 grams per liter verapamil.
Claim 13
Dependent Claim
101 words
An intracellular flush solution for preserving an organ for transplantation comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; (d) about 1.0 grams per liter adenosine; (e) about 2.3 grams per liter KHCO 3 ; (f) about 0.1 grams per liter allopurinol; and, (g) about 0.007 grams per liter verapamil.
Claim 14
Dependent Claim
45 words
An intracellular flush solution for preserving an organ for transplantation comprising a modified Collins C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 15
Dependent Claim
45 words
An intracellular flush solution for preserving an organ for transplantation comprising a modified Collins C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 16
Dependent Claim
65 words
An intracellular flush solution for preserving an organ for transplantation comprising a modified Collins C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 2.3 grams per liter KHCO 3 ; about 0.1 grams per liter allopurinol; and, about 0.007 grams per liter verapamil.
Claim 17
Dependent Claim
65 words
An intracellular flush solution for preserving an organ for transplantation comprising a modified Collins C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 2.3 grams per liter KHCO 3 ; about 0.1 grams per liter allopurinol; and, about 0.007 grams per liter verapamil.
Claim 18
Independent Claim
146 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 2.77 and 4.62 grams per liter of MgSO 4 ; (h) between about 0.75 and 1.25 grams per liter of adenosine; (i) between about 0 and 0.125 grams per liter of allopurinol; and, (j) between about 0 and 1.0 grams per liter of verapamil.
Claim 19
Independent Claim
146 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 2.77 and 4.62 grams per liter of MgSO 4 ; (h) between about 0.75 and 1.25 grams per liter of adenosine; (i) between about 0 and 0.125 grams per liter of allopurinol; and, (j) between about 0 and 1.0 grams per liter of verapamil.
Claim 20
Independent Claim
144 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 0.2 and 0.4 meq/ml of MgCl 2 ; (h) between about 0.75 and 1.25 grams per liter of adenosine; (i) between about 0 and 0.125 grams per liter of allopurinol; and, (j) between about 0 and 1.0 grams per liter of verapamil.
Claim 21
Independent Claim
144 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) between about 1.54 and 2.56 grams per liter of KH 2 PO 4 ; (b) between about 7.38 and 12.2 grams per liter of K 2 HPO 4 ; (c) between about 0.84 and 1.40 grams per liter of KCl; (d) between about 0.63 and 1.05 grams per liter of NaHCO 3 ; (e) between about 0 and 2.87 grams per liter of KHCO 3 ; (f) between about 20 and 37 grams per liter of mannitol; (g) between about 0.2 and 0.4 meq/ml of MgCl 2 ; (h) between about 0.75 and 1.25 grams per liter of adenosine; (i) between about 0 and 0.125 grams per liter of allopurinol; and, (j) between about 0 and 1.0 grams per liter of verapamil.
Claim 22
Independent Claim
76 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 23
Independent Claim
76 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 24
Independent Claim
93 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 2.3 grams per liter KHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 0.1 grams per liter allopurinol; and, about 0.007 verapamil.
Claim 25
Independent Claim
93 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising about 2.05 grams per liter KH 2 PO 4 ; about 9.7 grams per liter K 2 HPO 4 ; about 1.12 grams per liter KCl; about 0.84 grams per liter NaHCO 3 ; about 2.3 grams per liter KHCO 3 ; about 25.0 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 0.1 grams per liter allopurinol; and, about 0.007 verapamil.
Claim 26
Independent Claim
88 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; and, (d) about 1.0 grams per liter adenosine.
Claim 27
Independent Claim
88 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; and, (d) about 1.0 grams per liter adenosine.
Claim 28
Independent Claim
111 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; (d) about 1.0 grams per liter adenosine; (e) about 2.3 grams per liter KHCO 3 ; (f) about 0.1 grams per liter allopurinol; and, (g) about 0.007 grams per liter verapamil.
Claim 29
Independent Claim
111 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising: (a) a modified Collins C-2 solution containing about 2.05 grams per liter of KH 2 PO 4 ; about 9.70 grams per liter K 2 HPO 4 , about 1.12 grams per liter KCl; and, about 0.84 grams per liter NaHCO 3 ; (b) about 25 grams per liter mannitol; (c) about 3.7 grams per liter MgSO 4 ; (d) about 1.0 grams per liter adenosine; (e) about 2.3 grams per liter KHCO 3 ; (f) about 0.1 grams per liter allopurinol; and, (g) about 0.007 grams per liter verapamil.
Claim 30
Independent Claim
55 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising a modified Collins' C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 31
Independent Claim
55 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising a modified Collins' C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; and about 1.0 grams per liter adenosine.
Claim 32
Independent Claim
75 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising a modified Collins C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 2.3 grams per liter KHCO 3 ; about 0.1 grams per liter allopurinol; and, about 0.007 grams per liter verapamil.
Claim 33
Independent Claim
75 words
A method for preserving an organ for transplantation comprising flushing the organ to be transplanted with an intracellular flush solution comprising a modified Collins C-2 solution devoid of glucose and MgSO 4 ; and, about 25 grams per liter mannitol; about 3.7 grams per liter MgSO 4 ; about 1.0 grams per liter adenosine; about 2.3 grams per liter KHCO 3 ; about 0.1 grams per liter allopurinol; and, about 0.007 grams per liter verapamil.
References Cited (Backward Citations)
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