US3943993A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Low temperature storage of surface attached living cell cultures
Assignee: Individual
•
Filing: 1974-05-03
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Granted: 1976-03-16
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Expires: 1994-05-03
Abstract
Abstract A process is described in which surface attached living cell cultures are treated, cooled, stored and revived in such a manner as to retain their viability and their adherence to culture surfaces.
Inventors
- 👤 Kendall O. Smith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:29
CPC Classifications
A; A61; A61L; A61L2/00; A61L2/02; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/921; Y10S435/923
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
103 words
In a process for cooling, storing and reviving surface attached living cultured cells, the steps of: exposing living cultured cells which are attached to a surface of a storage vessel to a storage medium which is relatively non-toxic to such cells and which is compatible therewith during cooling to a temperature below 0° C, storage and subsequent warming processes; cooling said vessel and the surface attached living cells therein to a storage temperature below 0° C effective for arresting the metabolism of the cells; and storing said vessel and said surface attached cells at said storage temperature for an extended period of time.
Claim 2
Dependent Claim
103 words
In a process for cooling, storing and reviving surface attached living cultured cells, the steps of: exposing living cultured cells which are attached to a surface of a storage vessel to a storage medium which is relatively non-toxic to such cells and which is compatible therewith during cooling to a temperature below 0° C, storage and subsequent warming processes; cooling said vessel and the surface attached living cells therein to a storage temperature below 0° C effective for arresting the metabolism of the cells; and storing said vessel and said surface attached cells at said storage temperature for an extended period of time.
Claim 3
Dependent Claim
20 words
A process as set forth in claim 1 wherein said storage medium contains about 5 to about 40% dimethyl sulfoxide.
Claim 4
Dependent Claim
17 words
A process as set forth in claim 2 wherein said storage medium contains approximately 10% dimethyl sulfoxide.
Claim 5
Dependent Claim
22 words
A process as set forth in claim 3 wherein said cooling is effectuated at a rate of about 1° C per minute.
Claim 6
Dependent Claim
16 words
A process as set forth in claim 4 wherein said storage temperature is about -70° C.
Claim 7
Dependent Claim
16 words
A process as set forth in claim 2 wherein said storage medium contains approximately 10% glycerol.
Claim 8
Dependent Claim
22 words
A process as set forth in claim 6 wherein said cooling is effectuated at a rate of about 1° C per minute.
Claim 9
Dependent Claim
16 words
A process as set forth in claim 7 wherein said storage temperature is about -70° C.
Claim 10
Dependent Claim
17 words
A process as set forth in claim 2 wherein said storage medium contains approximately 10% ethylene glycol.
Claim 11
Dependent Claim
22 words
A process as set forth in claim 9 wherein said cooling is effectuated at a rate of about 1° C per minute.
Claim 12
Dependent Claim
16 words
A process as set forth in claim 10 wherein said storage temperature is about -70° C.
Claim 13
Dependent Claim
19 words
A process as set forth in claim 1 wherein said storage medium contains about 5 to about 40% glycerol.
Claim 14
Dependent Claim
20 words
A process as set forth in claim 1 wherein said storage medium contains about 5 to about 40% ethylene glycol.
Claim 15
Dependent Claim
22 words
A process as set forth in claim 1 wherein said cooling is effectuated at a rate of about 1° C per minute.
Claim 16
Dependent Claim
16 words
A process as set forth in claim 14 wherein said storage temperature is about -70° C.
Claim 17
Dependent Claim
16 words
A process as set forth in claim 1 wherein said storage temperature is about -70° C.
Claim 18
Dependent Claim
32 words
A process as set forth in claim 1 wherein said storage medium is present in at least a sufficient quantity to completely wet the outer surfaces of said surface attached living cells.
Claim 19
Dependent Claim
37 words
A process as set forth in claim 17 wherein said storage medium is present in that quantity only which adheres to said surface attached living cells by surface phenomena after decanting or aspiration of said storage vessel.
Claim 20
Dependent Claim
32 words
A process as set forth in claim 1 wherein said cooling comprises placing non-insulated storage vessels containing said surface attached living cells directly into a refrigerated space maintained at said storage temperature.
Claim 21
Dependent Claim
24 words
A process as set forth in claim 1 wherein said storage temperature is in the range of about 0° C to about -200° C.
Claim 22
Dependent Claim
29 words
A process as set forth in claim 1 wherein, subsequent to said storage step, said vessel and the surface attached cells therein are warmed sufficiently to thaw said cells.
References Cited (Backward Citations)
US3303662A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-03-16 | 1979-09-15 | 1980-03-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-03-16 | 1983-09-15 | 1984-03-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-03-16 | 1987-09-15 | 1988-03-16 | $7700.00 | Window Closed / Lapsed |