US4840034A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of freezing vital body fluids
Assignee: Individual
•
Filing: 1988-07-14
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Granted: 1989-06-20
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Expires: 2008-07-14
Abstract
Abstract A method of freezing vital body fluids for storage and later use is provided. The method includes the steps of preparing a brine including a cruciferous oil, cooling the brine, providing a heat transfer container having a vital body fluid therein and subjecting the heat transfer container to a heat transfer relationship with the cooled brine for a period of time sufficient to freeze the vital body fluid in the heat transfer container.
Inventors
- 👤 Barnet L. Liberman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:02
CPC Classifications
A; A23; A23B; A23B2/00; A23B2/80; A23B2/85; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
87 words
A method of freezing vital body fluids comprising: preparing a brine including at least 0.1% by weight of cruciferous oil; cooling the brine to a temperature between about -22° and -43.6° F.; providing a heat transfer container including a vital body fluid therein; and bringing the heat transfer container into a heat transfer relationship with the cooled brine for a period of time sufficient to transmit cold from the cooled brine to the vital body fluid in the heat transfer container and freeze the vital body fluid.
Claim 2
Independent Claim
87 words
A method of freezing vital body fluids comprising: preparing a brine including at least 0.1% by weight of cruciferous oil; cooling the brine to a temperature between about -22° and -43.6° F.; providing a heat transfer container including a vital body fluid therein; and bringing the heat transfer container into a heat transfer relationship with the cooled brine for a period of time sufficient to transmit cold from the cooled brine to the vital body fluid in the heat transfer container and freeze the vital body fluid.
Claim 3
Dependent Claim
22 words
The method of freezing vital body fluids of claim 1, wherein the oil is extracted from a plant of the genus Brassica.
Claim 4
Dependent Claim
27 words
The method of freezing vital body fluids of claim 1, wherein the oil is selected from the group consisting of rapeseed oil, mustard oil and mixtures thereof.
Claim 5
Dependent Claim
21 words
The method of freezing vital body fluids of claim 1, wherein the oil contains erucic acid as the single largest component.
Claim 6
Dependent Claim
20 words
The method of freezing vital body fluids of claim 1, wherein the oil contains less than about 2% saturated components.
Claim 7
Dependent Claim
28 words
The method of freezing vital body fluids of claim 1, wherein the oil is used in an amount between about 0.1 and 0.5% by weight of the brine.
Claim 8
Dependent Claim
22 words
The method of freezing vital body fluids of claim 1, wherein the brine further includes a glycol, an inorganic salt and water.
Claim 9
Dependent Claim
16 words
The method of freezing vital body fluids of claim 7, wherein the glycol is propylene glycol.
Claim 10
Dependent Claim
28 words
The method of freezing vital body fluids of claim 7, wherein the glycol is present in an amount between about 30 and 50% by weight of the brine.
Claim 11
Dependent Claim
16 words
The method of freezing vital body fluids of claim 7, wherein the salt is calcium chloride.
Claim 12
Dependent Claim
28 words
The method of freezing vital body fluids of claim 7, wherein the salt is present in an amount between about 40 and 60% by weight of the brine.
Claim 13
Dependent Claim
28 words
The method of freezing vital body fluids of claim 7, wherein the water is present in an amount between about 40 and 60% by weight of the brine.
Claim 14
Dependent Claim
27 words
The method of freezing vital body fluids of claim 1, wherein the vital body fluid is selected from the group consisting of sperm, eggs, zygotes and blood.
Claim 15
Dependent Claim
33 words
The method of freezing vital body fluids of claim 1, wherein the vital body fluid is brought into a heat transfer relationship with the brine for a period between about 1/2and 2 minutes.
Claim 16
Dependent Claim
39 words
The method of freezing vital body fluids of claim 1, wherein the vital body fluid is brought into a heat transfer relationship by immersion of the heat transfer container having the vital body fluid therein in the cooled brine.
Claim 17
Dependent Claim
53 words
The method of freezing vital body fluids of claim 1, wherein the vital body fluid is brought into a heat transfer relationship with the cooled brine by placing the heat transfer container having the vital body fluid therein in a heat transfer pan and subjecting the heat transfer pan to the cooled brine.
Claim 18
Dependent Claim
62 words
The method of freezing vital body fluids of claim 1, wherein the vital body fluid is brought into a heat transfer relationship by placing the heat transfer container having the vital body fluid therein into a heat transfer tray along with sufficient brine to at east coat the bottom of the tray and subjecting the heat transfer tray to the cooled brine.
Claim 19
Independent Claim
98 words
A method of freezing vital body fluids comprising: preparing a brine including an effective amount of a suitable oil for increasing the freezing rate of a vital body fluid brought into a heat transfer relationship therewith so as to minimize deterioration in the cellular structure of the body fluid; cooling the brine to a temperature between about -22° and -43.6° F.; providing a heat transfer container including a vital body fluid therein; bringing the heat transfer container into a heat transfer relationship with the cooled brine for a period of time sufficient to freeze the vital body fluid.
Claim 20
Independent Claim
98 words
A method of freezing vital body fluids comprising: preparing a brine including an effective amount of a suitable oil for increasing the freezing rate of a vital body fluid brought into a heat transfer relationship therewith so as to minimize deterioration in the cellular structure of the body fluid; cooling the brine to a temperature between about -22° and -43.6° F.; providing a heat transfer container including a vital body fluid therein; bringing the heat transfer container into a heat transfer relationship with the cooled brine for a period of time sufficient to freeze the vital body fluid.
Claim 21
Dependent Claim
37 words
A method of freezing vital body fluids of claim 18, wherein the brine includes between about 0.1 and 1.0% by weight of an oil selected from the group consisting of rapeseed oil, mustard oil and mixtures thereof.
Claim 22
Dependent Claim
38 words
A method of freezing vital body fluids of claim 18, wherein the brine further includes about 30 and 50% by weight of a glycol, between about 5 and 15% by weight of salt and a balance of water.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-06-19 | 1992-12-19 | 1993-06-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-06-19 | 1996-12-19 | 1997-06-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-06-19 | 2000-12-19 | 2001-06-20 | $7700.00 | Window Closed / Lapsed |