US5328821A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cold and cryo-preservation methods for human tissue slices
Assignee: Individual
•
Filing: 1991-12-12
•
Granted: 1994-07-12
•
Expires: 2011-12-12
Abstract
Abstract Cold-and cryo-preservation solutions for tissue slices include a limited amount of glucose. The solutions provide extended cold storage of the slices without a loss of viability. The solutions also allow cryopreservation of the slices, so that the slices may be stored until needed and then thawed, cultured, packaged and distributed.
Inventors
- 👤 Robyn Fisher
- 👤 Klaus Brendel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:22
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A01N1/125
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
71 words
A method for the cold preservation of organ tissue comprising storing said tissue in an aqueous preservation solution comprising: 1-5 mM glucose; 30-120 mM gluconate; 30-120 mM saccharate; 25-30 mM potassium phosphate(monobasic); 1-3 mM sodium succinate; 3-6 mM magnesium sulfate; 1-3 mM magnesium chloride; 1-3 mM potassium bicarbonate; 2-4 mM fructose; 3-6 mM glutathione; 2-10 mM ascorbic acid; 3-5 mM adenosine and micromolar amounts of deferoxamine mesylate and gentamicin or penicillin.
Claim 2
Independent Claim
71 words
A method for the cold preservation of organ tissue comprising storing said tissue in an aqueous preservation solution comprising: 1-5 mM glucose; 30-120 mM gluconate; 30-120 mM saccharate; 25-30 mM potassium phosphate(monobasic); 1-3 mM sodium succinate; 3-6 mM magnesium sulfate; 1-3 mM magnesium chloride; 1-3 mM potassium bicarbonate; 2-4 mM fructose; 3-6 mM glutathione; 2-10 mM ascorbic acid; 3-5 mM adenosine and micromolar amounts of deferoxamine mesylate and gentamicin or penicillin.
Claim 3
Independent Claim
24 words
The method of 1 wherein the solution further comprises a cryoprotectant and the method further comprises immersing the preserved tissue into a crygenic fluid.
Claim 4
Independent Claim
24 words
The method of 1 wherein the solution further comprises a cryoprotectant and the method further comprises immersing the preserved tissue into a crygenic fluid.
Claim 5
Dependent Claim
22 words
The method of claim 2 wherein said cryoprotectant is selected from the group consisting of dimethyl sulfoxide, glycerol, ethylene glycol and propanediol.
Claim 6
Dependent Claim
26 words
The method of claim 2 further comprising the step of cooling the tissue at a controlled rate prior to immersing the tissue in the cryogenic fluid.
Claim 7
Dependent Claim
20 words
The method of claim 2 wherein said cryoprotectant comprises from about 5% to about 50% by weight of said solution.
Claim 8
Dependent Claim
34 words
The method of claim 2 wherein said storing step comprises sequentially placing the tissue in a series of solutions, each solution in the sequence having a greater concentration of cryoprotectant than the previous solution.
Claim 9
Dependent Claim
16 words
The method of claim 2 further comprising the step of thawing the cryopreserved human tissue slices.
References Cited (Backward Citations)
US3551290A
Cited
US3431172A
Cited
US3458397A
Cited
US3682776A
Cited
US3676298A
Cited
US3892628A
Cited
US3753357A
Cited
US3914954A
Cited
US3935065A
Cited
US3881990A
Cited
US3766015A
Cited
US3983712A
Cited
US3943993A
Cited
US3912809A
Cited
US4008754A
Cited
US3936269A
Cited
US4018911A
Cited
US4107937A
Cited
US4004975A
Cited
US4030314A
Cited
US4040785A
Cited
US4155331A
Cited
US4117881A
Cited
US4473552A
Cited
US4353930A
Cited
US4494385A
Cited
US4681839A
Cited
US4473637A
Cited
US4559298A
Cited
US4837390A
Cited
US4471629A
Cited
US4609622A
Cited
US4865871A
Cited
US4799361A
Cited
US4695536A
Cited
US4565643A
Cited
US4727018A
Cited
US4959319A
Cited
US4601909A
Cited
US4798824A
Cited
US4879283A
Cited
US4688387A
Cited
US4695460A
Cited
US4812310A
Cited
US4745759A
Cited
US4890457A
Cited
US4978546A
Cited
US5145769A
Cited
US5158867A
Cited
US4873186A
Cited
US4980277A
Cited
US4956272A
Cited
US4978540A
Cited
US4832972A
Cited
US4965186A
Cited
US4840034A
Cited
US4840035A
Cited
US4920044A
Cited
US4931361A
Cited
US4940599A
Cited
US5145771A
Cited
US5145770A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-07-11 | 1998-01-10 | 1998-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-07-11 | 2002-01-10 | 2002-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-07-11 | 2006-01-10 | 2006-07-12 | $7700.00 | Window Closed / Lapsed |