Patent US1715677A successfully refreshed from Google Patents.
US6025136A
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Methods and apparatus for DNA sequencing and DNA identification
Assignee: Hyseq Inc
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Filing: 1997-08-28
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Granted: 2000-02-15
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Expires: 2017-08-28
Abstract
Abstract Sequencing by Hybridization (SBH) methods and apparatus employing subdivided filters for discrete multiple probe analysis of multiple samples may be used for DNA identification and for DNA sequencing. Partitioned filters are prepared. Samples are affixed to sections of partitioned filters and each sector is probed with a single probe or a multiplexed probe for hybridization scoring. Hybridization data is analyzed for probe complementarity, partial sequencing by SBH or complete sequencing by SBH.
Inventors
- 👤 Radoje Drmanac
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 07:14
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; B; B01; B01J; B01J19/00; B01J19/0046; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12Q1/6813; C12Q1/6827; C12Q1/6869; C12Q1/6874; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00351; B01J2219/00364; B01J2219/00367; B01J2219/00369; B01J2219/00378; B01J2219/00387; B01J2219/00497; B01J2219/00504; B01J2219/00513; B01J2219/00527; B01J2219/00583; B01J2219/00603; B01J2219/00659; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/582
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
191 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a first plurality of nucleic acid segments on a first sector of a substrate; disposing a second plurality of nucleic acid segments on a second sector of the substrate; introducing a barrier to movement of nucleic acid by applying a direction-switching electrical field perpendicular to the plane of the sectors to prevent mixing of nucleic acids between the sectors; exposing, under conditions discriminating between full complementarity and a one base mismatch, the first plurality of nucleic acid segments to a first hybridization probe in the first sector, the first hybridization probe being shorter than one from among the first plurality of nucleic acid segments, to the plurality of nucleic acid segments; incubating under conditions discriminating between full complementarity and a one base mismatch, a second hybridization probe in the second sector, the second hybridization probe being shorter than a segment from among the second plurality of nucleic acid segments and the second hybridization probe being different in sequence from the first hybridization probe; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 2
Independent Claim
191 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a first plurality of nucleic acid segments on a first sector of a substrate; disposing a second plurality of nucleic acid segments on a second sector of the substrate; introducing a barrier to movement of nucleic acid by applying a direction-switching electrical field perpendicular to the plane of the sectors to prevent mixing of nucleic acids between the sectors; exposing, under conditions discriminating between full complementarity and a one base mismatch, the first plurality of nucleic acid segments to a first hybridization probe in the first sector, the first hybridization probe being shorter than one from among the first plurality of nucleic acid segments, to the plurality of nucleic acid segments; incubating under conditions discriminating between full complementarity and a one base mismatch, a second hybridization probe in the second sector, the second hybridization probe being shorter than a segment from among the second plurality of nucleic acid segments and the second hybridization probe being different in sequence from the first hybridization probe; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 3
Independent Claim
174 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a first plurality of nucleic acid segments on a first sector of a substrate; disposing a second plurality of nucleic acid segments on a second sector of the substrate; introducing a barrier to movement of nucleic acid by applying an electric field; exposing, under conditions discriminating between full complementarity and a one base mismatch, the first plurality of nucleic acid segments to a first hybridization probe in the first sector, the first hybridization probe being shorter than one from among the first plurality of nucleic acid segments, to the plurality of nucleic acid segments; incubating under conditions discriminating between full complementarity and a one base mismatch, a second hybridization probe in the second sector, the second hybridization probe being shorter than a segment from among the second plurality of nucleic acid segments and the second hybridization probe being different in sequence from the first hybridization probe; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 4
Independent Claim
174 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a first plurality of nucleic acid segments on a first sector of a substrate; disposing a second plurality of nucleic acid segments on a second sector of the substrate; introducing a barrier to movement of nucleic acid by applying an electric field; exposing, under conditions discriminating between full complementarity and a one base mismatch, the first plurality of nucleic acid segments to a first hybridization probe in the first sector, the first hybridization probe being shorter than one from among the first plurality of nucleic acid segments, to the plurality of nucleic acid segments; incubating under conditions discriminating between full complementarity and a one base mismatch, a second hybridization probe in the second sector, the second hybridization probe being shorter than a segment from among the second plurality of nucleic acid segments and the second hybridization probe being different in sequence from the first hybridization probe; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 5
Dependent Claim
12 words
The method of claim 2 wherein the electric field is direction switching.
Claim 6
Dependent Claim
18 words
The method of claim 2 wherein the electric field is applied perpendicular to the plane of the substrate.
Claim 7
Dependent Claim
20 words
The method of claim 2 wherein the electric field is applied to prevent mixing of nucleic acids between the sectors.
Claim 8
Independent Claim
114 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of nucleic acid segments on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying a direction-switching electrical field perpendicular to the plane of the sector to prevent movement of nucleic acids; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of nucleic acid segments with a hybridization probe in the sector, the hybridization probe being shorter than one from among the plurality of nucleic acid segments, to the plurality of nucleic acid segments; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 9
Independent Claim
114 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of nucleic acid segments on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying a direction-switching electrical field perpendicular to the plane of the sector to prevent movement of nucleic acids; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of nucleic acid segments with a hybridization probe in the sector, the hybridization probe being shorter than one from among the plurality of nucleic acid segments, to the plurality of nucleic acid segments; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 10
Independent Claim
100 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of nucleic acid segments on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying an electric field; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of nucleic acid segments with a hybridization probe in the sector, the hybridization probe being shorter than one from among the plurality of nucleic acid segments, to the plurality of nucleic acid segments; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 11
Independent Claim
100 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of nucleic acid segments on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying an electric field; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of nucleic acid segments with a hybridization probe in the sector, the hybridization probe being shorter than one from among the plurality of nucleic acid segments, to the plurality of nucleic acid segments; detecting hybridization of a hybridization probe to a nucleic acid segment; and analyzing the result.
Claim 12
Dependent Claim
12 words
The method of claim 7 wherein the electric field is direction switching.
Claim 13
Dependent Claim
18 words
The method of claim 7 wherein the electric field is applied perpendicular to the plane of the substrate.
Claim 14
Dependent Claim
17 words
The method of claim 7 wherein the electric field is applied to prevent movement of nucleic acids.
Claim 15
Independent Claim
100 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of hybridization probes on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying a direction-switching electrical field perpendicular to the plane of the sector to prevent movement of nucleic acids; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of hybridization probes in the sector to a nucleic acid segment, the hybridization probes being shorter than the nucleic acid segment; detecting hybridization of hybridization probes with the nucleic acid segment; and analyzing the result.
Claim 16
Independent Claim
100 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of hybridization probes on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying a direction-switching electrical field perpendicular to the plane of the sector to prevent movement of nucleic acids; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of hybridization probes in the sector to a nucleic acid segment, the hybridization probes being shorter than the nucleic acid segment; detecting hybridization of hybridization probes with the nucleic acid segment; and analyzing the result.
Claim 17
Independent Claim
86 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of hybridization probes on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying an electric field; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of hybridization probes in the sector to a nucleic acid segment, the hybridization probes being shorter than the nucleic acid segment; detecting hybridization of hybridization probes to the nucleic acid segment; and analyzing the result.
Claim 18
Independent Claim
86 words
A method for analyzing nucleic acids by hybridization, comprising the steps of: arraying a plurality of hybridization probes on a sector of a substrate; introducing a barrier to movement of nucleic acid by applying an electric field; incubating under conditions discriminating between full complementarity and a one base mismatch, the plurality of hybridization probes in the sector to a nucleic acid segment, the hybridization probes being shorter than the nucleic acid segment; detecting hybridization of hybridization probes to the nucleic acid segment; and analyzing the result.
Claim 19
Dependent Claim
12 words
The method of claim 12 wherein the electric field is direction switching.
Claim 20
Dependent Claim
18 words
The method of claim 12 wherein the electric field is applied perpendicular to the plane of the substrate.
Claim 21
Dependent Claim
17 words
The method of claim 12 wherein the electric field is applied to prevent movement of nucleic acids.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-02-14 | 2003-08-16 | 2004-02-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-02-14 | 2007-08-16 | 2008-02-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-02-14 | 2011-08-16 | 2012-02-15 | $7700.00 | Window Closed / Lapsed |