US4806546A
Expired / Lapsed
US
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Immobilization of nucleic acids on derivatized nylon supports
Assignee: Bayer Corp
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Filing: 1985-09-30
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Granted: 1989-02-21
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Expires: 2005-09-30
Abstract
Abstract Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon having amide groups that have been derivatized to amidine residues. Derivatization of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by reaction with an amine. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample.
Inventors
- 👤 Robert J. Carrico
- 👤 William L. Patterson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:13
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
65 words
A method for immobilizing a nucleic acid consisting essentially of the step of contacting the nucleic acid with a solid support consisting essentially of nylon having amide groups which have been derivatized to amidine residues of the formula: ##STR2## wherein R is alkylene, pheylene, alkyphenylene, or phenalkylene, and X is a counteranion, whereby said nucleic acid becomes bound to the solid support by noncovalent bonds.
Claim 2
Independent Claim
65 words
A method for immobilizing a nucleic acid consisting essentially of the step of contacting the nucleic acid with a solid support consisting essentially of nylon having amide groups which have been derivatized to amidine residues of the formula: ##STR2## wherein R is alkylene, pheylene, alkyphenylene, or phenalkylene, and X is a counteranion, whereby said nucleic acid becomes bound to the solid support by noncovalent bonds.
Claim 3
Dependent Claim
27 words
The method of claim 1 wherein the nylon has been treated to activate the amide groups therein and reacted with an amine to introduce said amidine residues.
Claim 4
Dependent Claim
17 words
The method of claim 2 wherein the activation and amine reaction steps are performed under anhydrous conditions.
Claim 5
Dependent Claim
11 words
The method of claim 1 whrein the nucleic acid is RNA.
Claim 6
Dependent Claim
18 words
The method of claim 2 wherein the amine is an α,ω-alkanediamine having between 2 and 12 carbon atoms.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the nucleic acid is DNA.
Claim 8
Dependent Claim
16 words
The method of claim 1 wherein the solid support is in the shape of a bead.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-21 | 1992-08-22 | 1993-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-21 | 1996-08-22 | 1997-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-21 | 2000-08-22 | 2001-02-21 | $7700.00 | Window Closed / Lapsed |