US4115305A
Expired / Lapsed
US
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Support matrix for carrying biologically active materials and process for the preparation thereof
Assignee: National Research Development Corp UK
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Filing: 1976-06-02
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Granted: 1978-09-19
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Expires: 1996-06-02
Abstract
Abstract A support for a biologically active material which comprises an organic polymer incorporating amidine groups the carbon atoms of which are bound to side chains each of which includes a functional group capable of linkage to a biologically active material, and a carbonyl group or an enol form thereof which is spaced from the carbon atom of an amidine group by the backbone dependent nitrogen atom thereof and at most by one other nitrogen atom in the side chain.
Inventors
- 👤 William Edward Hornby
- 👤 David Lindsey Morris
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 05:11
CPC Classifications
C; C08; C08J; C08J7/00; C08J7/12; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/089; C12N11/096; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
89 words
A biologically active matrix comprising a support and a biologically active material which is bound thereto, said support comprising an organic polymer incorporating amidine groups the carbon atoms of which are bound to side chains each of which includes a functional group capable of linkage to the biologically active material, and a carbonyl group or an enol form thereof which is spaced from the carbon atom of an amidine group by the backbone dependent nitrogen atom thereof and at most by one other nitrogen atom in the side chain.
Claim 2
Dependent Claim
89 words
A biologically active matrix comprising a support and a biologically active material which is bound thereto, said support comprising an organic polymer incorporating amidine groups the carbon atoms of which are bound to side chains each of which includes a functional group capable of linkage to the biologically active material, and a carbonyl group or an enol form thereof which is spaced from the carbon atom of an amidine group by the backbone dependent nitrogen atom thereof and at most by one other nitrogen atom in the side chain.
Claim 3
Dependent Claim
14 words
A matrix according to claim 1, in which the polymer is a modified nylon.
Claim 4
Dependent Claim
61 words
A matrix according to claim 1, in which the side chains include a group A which exists in one or more of the tautomeric forms --N 1 HN 2 HCO--, ═N 1 --N 2 HCO, --N 1 H--N 2 ═C(OH)--, and ═N 1 --N 2 ═C(OH)--, N 1 representing the dependent nitrogen atom of an amidine group incorporated in the polymer.
Claim 5
Dependent Claim
23 words
A matrix according to claim 3, in which said group A is directly linked to the group --R--CO--, R representing an organic radical.
Claim 6
Dependent Claim
22 words
A matrix according to claim 4, in which side chains include the group --CORCO-- which represents succinyl, oxalyl, adipoyl, phthaloyl or dipicolinoyl.
Claim 7
Dependent Claim
28 words
A matrix according to claim 1, in which the side chain comprises a polysaccharide carrying a plurality of functional groups capable of linkage to the biologically active material.
Claim 8
Dependent Claim
31 words
A matrix according to claim 6, in which the polysaccharide is linked to the carbon atoms of the amidine groups by the group --A--R--CO--NH--NH-- in which R represents an organic radical.
Claim 9
Dependent Claim
14 words
A matrix according to claim 6, in which the polysaccharide is an oxidised dextran.
Claim 10
Dependent Claim
48 words
A matrix according to claim 3, in which the side chain comprises a polysaccharide which carries a plurality of functional groups capable of linkage to the biologically active material and which is linked to the carbon atoms of the amidine groups in the polymer by the group A.
Claim 11
Dependent Claim
12 words
A matrix according to claim 9, in which the polysaccharide is pectin.
Claim 12
Dependent Claim
19 words
A matrix according to claim 1, in which said functional groups are aldehyde, azide, adipimidate, suberimidate or glutaraldehyde groups.
Claim 13
Dependent Claim
15 words
A matrix according to claim 1, in which the biologically active material is a protein.
Claim 14
Dependent Claim
21 words
A matrix according to claim 12, in which the biologically active material is an enzyme, a co-factor, inhibitor, antigen or antibody.
Claim 15
Dependent Claim
46 words
An intermediate for the production of a support for a biologically active material which comprises an organic polymer incorporating amidine groups the carbon atoms of which are bound to side chains comprising an acid dihydrazide which includes the backbone dependent nitrogen atom of the amidine groups.
Claim 16
Dependent Claim
46 words
An intermediate for the production of a support for a biologically active material which comprises an organic polymer incorporating amidine groups the carbon atoms of which are bound to side chains comprising an acid dihydrazide which includes the backbone dependent nitrogen atom of the amidine groups.
Claim 17
Dependent Claim
14 words
An intermediate according to claim 14, in which the polymer is a modified nylon.
Claim 18
Dependent Claim
24 words
An intermediate according to claim 14, in which the dihydrazide is of a succinic acid, oxalic acid, adipic acid, phthalic acid or dipicolinic acid.
Claim 19
Dependent Claim
19 words
An intermediate according to claim 14, in which the side chain comprises a hydrazide of oxidised dextran or pectin.
Claim 20
Dependent Claim
82 words
A process for the production of a support for a biologically active material in which imidate groups of an organic polymer are reacted with an organic compound so as to introduce into the polymer side chains which include a carbonyl group or an enol form thereof spaced from the polymer backbone by one or two nitrogen atoms of the side chain, and the side chains are then modified so as to comprise functional groups capable of linkage to a biologically active material.
Claim 21
Dependent Claim
82 words
A process for the production of a support for a biologically active material in which imidate groups of an organic polymer are reacted with an organic compound so as to introduce into the polymer side chains which include a carbonyl group or an enol form thereof spaced from the polymer backbone by one or two nitrogen atoms of the side chain, and the side chains are then modified so as to comprise functional groups capable of linkage to a biologically active material.
Claim 22
Dependent Claim
14 words
A process according to claim 18, in which the polymer is a modified nylon.
Claim 23
Dependent Claim
15 words
A process according to claim 18, in which the organic compound is an acid dihydrazide.
Claim 24
Dependent Claim
19 words
A process according to claim 20 in which the dihydrazide is a succinic, oxalic, adipic, phthalic or dipicolinic acid.
Claim 25
Dependent Claim
14 words
A process according to claim 20, in which the organic compound comprises a polysaccharide.
Claim 26
Dependent Claim
28 words
A process according to claim 18, in which the reaction is carried out in an at least predominantly non aqueous solvent at a compound concentration at least 50mM.
Claim 27
Dependent Claim
25 words
A process according to claim 18, in which the side chains are modified by conversion of a --CONHNH 2 group therein to an azide group.
Claim 28
Dependent Claim
28 words
A process according to claim 18, in which the side chains are modified by reaction of a --CO NH NH 2 group therein with glutaraldehyde, adipimidate or suberimidate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-09-18 | 1982-03-20 | 1982-09-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-09-18 | 1986-03-20 | 1986-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-09-18 | 1990-03-20 | 1990-09-19 | $7700.00 | Window Closed / Lapsed |