US3969287A
Expired / Lapsed
US
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Carrier-bound protein prepared by reacting the protein with an acylating or alkylating compound having a carrier-bonding group and reacting the product with a carrier
Assignee: Roche Diagnostics GmbH
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Filing: 1973-11-30
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Granted: 1976-07-13
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Expires: 1993-11-30
Abstract
Abstract Carrier-bound proteins are prepared by reacting a protein in aqueous solution with a coupling compound having at least one group capable of acylating or alkylating proteins and at least one additional group capable of producing a bond with a carrier material, and reacting the resulting product with a carrier material, optionally forming the carrier material in situ by polymerization of a monomer or monomer mixture in the presence of said product.
Inventors
- 👤 Dieter Jaworek
- 👤 Michael Nelbock-Hochstetter
- 👤 Klaus Beaucamp
- 👤 Hans Ulrich Bergmeyer
- 👤 Karl-Heinz Botsch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:48
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/18; C08; C08F; C08F289/00; C12N11/02; C12N11/06; C12N11/08; C12N11/082; C12N11/087; C12N11/089; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
90 words
Process for the preparation of a carrier-bound protein, which process comprises reacting a protein in aqueous solution with a compound containing at least one group capable of acylating or alkylating proteins, said group being selected from the class consisting of an ethyleneimine group, a halide group activated by unsaturation, an acid-halide group, an azide group, an acid anhydride group, an aldehyde group, and an oxazolone group; and at least one additional group capable of producing a bond with a carrier material, and reacting the reducing product with a carrier material.
Claim 2
Dependent Claim
90 words
Process for the preparation of a carrier-bound protein, which process comprises reacting a protein in aqueous solution with a compound containing at least one group capable of acylating or alkylating proteins, said group being selected from the class consisting of an ethyleneimine group, a halide group activated by unsaturation, an acid-halide group, an azide group, an acid anhydride group, an aldehyde group, and an oxazolone group; and at least one additional group capable of producing a bond with a carrier material, and reacting the reducing product with a carrier material.
Claim 3
Dependent Claim
19 words
Process as claimed in claim 1, wherein said group capable of acylating or alkylating proteins is an ethyleneimine group.
Claim 4
Dependent Claim
22 words
Process as claimed in claim 1, wherein said group capable of acylating or alkylating proteins is a halide group activated by unsaturation.
Claim 5
Dependent Claim
20 words
Process as claimed in claim 1, wherein said group capable of acylating or alkylating proteins is an acid halide group.
Claim 6
Dependent Claim
19 words
Process as claimed in claim 1, wherein said group capable of acylating or alkylating proteins is an azide group.
Claim 7
Dependent Claim
20 words
Process as claimed in claim 1, wherein said group capable of acylating or alkylating proteins is an acid anhydride group.
Claim 8
Dependent Claim
19 words
Process as claimed in claim 1, wherein said group capable of acylating or alkylating proteins is an aldehyde group.
Claim 9
Dependent Claim
19 words
Process as claimed in claim 1, wherein said group capable of acylating or alkylating proteins is an oxazolone group.
Claim 10
Dependent Claim
24 words
Process as claimed in claim 1, wherein said additional group capable of producing a bond with a carrier material is a copolymerizable double bond.
Claim 11
Dependent Claim
23 words
Process as claimed in claim 1, wherein said carrier material is produced in aqueous solution by the polymerization of at least one monomer.
Claim 12
Dependent Claim
44 words
Process as claimed in claim 10, wherein the polymerizable monomer or monomer mixture is added to the aqueous solution in which said protein was reacted, subsequent to the reaction of the protein with the coupling compound, and then polymerizing said monomer or monomer mixture.
Claim 13
Dependent Claim
36 words
Process as claimed in claim 10, wherein the monomer is selected from the group consisting of the water-soluble amide, nitrile or ester derivative of vinyl alcohol, acrylic acid or methacrylic acid and mixtures of said derivatives.
Claim 14
Dependent Claim
47 words
Process as claimed in claim 1, wherein the reaction of the protein with said compound containing said groups is effected in the presence of a polymerizable monomer or monomer mixture, and then the polymerization is carried out to incorporate into the forming polymer the protein-compound reaction product.
Claim 15
Dependent Claim
12 words
Process as claimed in claim 1, wherein said carrier material is cross-linked.
Claim 16
Dependent Claim
63 words
Process as claimed in claim 1, wherein said compound is of the formula R.CO.X, wherein X is selected from the group consisting of ##EQU3## wherein R 1 is alkyl of up to 6 carbon atoms, R 2 is aryl or aralkyl of up to 12 carbon atoms or halogen atom, and R 3 is alkyl of up to 6 carbon atoms or hydrogen.
Claim 17
Dependent Claim
44 words
Process as claimed in claim 1, wherein said compound is of the formula R.CO.X, wherein X is selected from the group consisting of ##EQU4## wherein R is the residue of a coupling compound which contains a functional group capable of bonding with the carrier.
Claim 18
Dependent Claim
10 words
Carrier-bound protein produced by the process claimed in claim 1.
References Cited (Backward Citations)
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US3788948A
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US3764477A
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US3759890A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-07-13 | 1980-01-12 | 1980-07-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-07-13 | 1984-01-12 | 1984-07-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-07-13 | 1988-01-12 | 1988-07-13 | $7700.00 | Window Closed / Lapsed |