US5436327A
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Support-bound oligonucleotides
Assignee: Oxford Gene Technology Ltd
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Filing: 1989-09-21
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Granted: 1995-07-25
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Expires: 2009-09-21
Abstract
Abstract PCT No. PCT/GB89/01114 Sec. 371 Date Mar. 20, 1991 Sec. 102(e) Date Mar. 20, 1991 PCT Filed Sep. 21, 1989 PCT Pub. No. WO90/03382 PCT Pub. Date Apr. 5, 1990.A method for synthesizing oligonucleotides by solid-phase methodology wherein the linkage between the oligonucleotide and the solid phase support is a non-labile phosphodiester. The spacer arm between the support and the phosphodiester is a hydrophilic group. The product of this method is an immobilized oligonucleotide which can be used in solid phase hybridization assays.
Inventors
- 👤 Edwin M. Southern
- 👤 Uwe Maskos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:03
CPC Classifications
C; C07; C07H; C07H21/00; C40; C40B; C40B40/00; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
141 words
In a method of preparing an oligonucleotide bound to a support by a) attaching to a support a 2'-deoxynucleoside reagent selected from the group consisting of nucleoside 3' reagents and nucleoside 5' reagents which reagent is selected from the group consisting of phosphite, phosphonate, hydrogen phosphonate, phosphonamidite, phosphoramidite and methylphosphonamidite reagents, b) synthesizing on the supported nucleoside an oligonucleotide chain including protecting groups, and c) removing the protecting groups from the oligonucleotide chain, wherein the support carries hydroxyl groups, whereby in step a) the nucleoside becomes attached to the support by a covalent phosphodiester link which is stable to the conditions used in step c), the improvement wherein the groups carried by the support have the formula --ROH, where R is selected from the group consisting of alkoxy, poly(alkoxy) and --(C n H 2n )-- wherein n is at least 3.
Claim 2
Dependent Claim
141 words
In a method of preparing an oligonucleotide bound to a support by a) attaching to a support a 2'-deoxynucleoside reagent selected from the group consisting of nucleoside 3' reagents and nucleoside 5' reagents which reagent is selected from the group consisting of phosphite, phosphonate, hydrogen phosphonate, phosphonamidite, phosphoramidite and methylphosphonamidite reagents, b) synthesizing on the supported nucleoside an oligonucleotide chain including protecting groups, and c) removing the protecting groups from the oligonucleotide chain, wherein the support carries hydroxyl groups, whereby in step a) the nucleoside becomes attached to the support by a covalent phosphodiester link which is stable to the conditions used in step c), the improvement wherein the groups carried by the support have the formula --ROH, where R is selected from the group consisting of alkoxy, poly(alkoxy) and --(C n H 2n )-- wherein n is at least 3.
Claim 3
Dependent Claim
17 words
The method as claimed in claim 1, wherein the support is of porous glass or glass beads.
Claim 4
Dependent Claim
15 words
The method as claimed in claim 1, wherein the nucleoside reagent is a nucleoside 3'-phosphoramidite.
Claim 5
Dependent Claim
15 words
The method as claimed in claim 1, wherein the nucleoside reagent is a nucleoside 3'-phosphite.
Claim 6
Dependent Claim
18 words
The method as claimed in claim 1, wherein the nucleoside reagent is a nucleoside 3'-phosphonate or 3'-hydrogen phosphonate.
Claim 7
Dependent Claim
15 words
The method as claimed in claim 1, wherein the nucleoside reagent is a nucleoside 3'-methylphosphonamidite.
Claim 8
Dependent Claim
36 words
The method as claimed in claim 1 wherein step c) involves removing protecting groups from phosphotriester groups and from N atoms of the nucleotide bases and from the 5'-position on the last nucleotide of the chain.
Claim 9
Dependent Claim
21 words
The method of claim 1, wherein the covalent phosphodiester link is --O--PY--O--R--, where Y is a protected or unprotected oxygen atom.
References Cited (Backward Citations)
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