US4775631A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of localizing nucleic acids bound to polyamide supports
Assignee: Janssen Pharmaceutica NV
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Filing: 1985-07-26
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Granted: 1988-10-04
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Expires: 2005-07-26
Abstract
Abstract A method of detecting and localizing nucleic acids on polyamide supports which comprises contacting said supports with a suspension of colloidal metal particles whereupon the metal particles bind to the polyamide support and form a colored background against which the location where nucleic acids are present become visible as lighter spots. The method enables the mere qualitative localization of the nucleic acids and the quantitative determination thereof following art-known procedures.
Inventors
- 👤 Eric L. J. Saman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 15:33
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; C12Q1/6816
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
50 words
A method of localizing nucleic acids on polyamide supports which comprises contacting said supports with an aqueous suspension of colloidal metal particles whereupon the metal particles bind to the polyamide support and form a coloured background against which the locations where nucleic acids are present become visible as lighter spots.
Claim 2
Independent Claim
50 words
A method of localizing nucleic acids on polyamide supports which comprises contacting said supports with an aqueous suspension of colloidal metal particles whereupon the metal particles bind to the polyamide support and form a coloured background against which the locations where nucleic acids are present become visible as lighter spots.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein the colloidal metal particles are gold particles.
Claim 4
Dependent Claim
19 words
A method according to claim 2 wherein the gold particles have a mean diameter between 1 and 100 nm.
Claim 5
Independent Claim
19 words
A method according to any one of claims 1 to 3 wherein the said nucleic acids are deoxyribonucleic acids.
Claim 6
Independent Claim
19 words
A method according to any one of claims 1 to 3 wherein the said nucleic acids are ribonucleic acids.
Claim 7
Independent Claim
20 words
A method according to any one of claims 1 to 3 wherein the said polyamide support is a polyhexamethylene adipamine.
Claim 8
Dependent Claim
15 words
A method according to claim 1 wherein the polyamide support is a nylon based membrane.
Claim 9
Dependent Claim
18 words
A method according to claim 7 wherein the colloidal metal particles are gold, platinum, silver, or copper particles.
References Cited (Backward Citations)
US4455370A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-04 | 1992-04-04 | 1992-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-04 | 1996-04-04 | 1996-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-04 | 2000-04-04 | 2000-10-04 | $7700.00 | Window Closed / Lapsed |