US4455370A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Transferring separated components in gel electrophoresis via nylon membrane
Assignee: Revvity Health Sciences Inc
•
Filing: 1983-03-07
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Granted: 1984-06-19
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Expires: 2003-03-07
Abstract
Abstract Microporous nylon films used as transfer membranes for nucleic acids, proteins, bacteria and viruses.
Inventors
- 👤 Bart W. Bartelsman
- 👤 Dennis L. Fost
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:54
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
33 words
In methods for transferring nucleic acids, proteins, bacteria or viruses from gel or culture media to a microporous adsorptive membrane, the improvement which comprises using as the membrane a microporous adsorptive nylon membrane.
Claim 2
Dependent Claim
33 words
In methods for transferring nucleic acids, proteins, bacteria or viruses from gel or culture media to a microporous adsorptive membrane, the improvement which comprises using as the membrane a microporous adsorptive nylon membrane.
Claim 3
Dependent Claim
21 words
Method of claim 1 wherein nucleic acids or proteins are transferred from an electrophoresis gel to a microporous adsorptive nylon membrane.
Claim 4
Dependent Claim
11 words
Method of claim 2 wherein the transfer is accomplished by blotting.
Claim 5
Dependent Claim
11 words
Method of claim 2 wherein the transfer is accomplished by electroblotting.
Claim 6
Dependent Claim
17 words
Method of claim 1, 2, 3 or 4 in which the membrane is a microporous 66-nylon membrane.
Claim 7
Dependent Claim
26 words
Method of claim 2, 3 or 4 wherein denatured DNA is transferred to the membrane and hybridized on the membrane with a labeled denatured DNA probe.
Claim 8
Dependent Claim
26 words
Method of claim 6 wherein the probe is removed by denaturation and the transferred DNA is rehybridized on the same membrane with another labeled DNA probe.
Claim 9
Dependent Claim
22 words
Method of claim 4 wherein RNA is transferred to the membrane and hybridized on the membrane with a labeled denatured DNA probe.
Claim 10
Dependent Claim
26 words
Method of claim 1 wherein nucleic acids, proteins, bacteria or viruses are transferred from a culture medium by contacting the medium with a microporous nylon membrane.
Claim 11
Dependent Claim
27 words
Method of claim 9 wherein bacteria are lifted from a colony in a culture medium by contact with a microporous 66-nylon membrane laminated to a support sheet.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-19 | 1987-12-19 | 1988-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-19 | 1991-12-19 | 1992-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-19 | 1995-12-19 | 1996-06-19 | $7700.00 | Window Closed / Lapsed |