US4806631A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of nucleic acids on solvolyzed nylon supports
Assignee: Bayer Corp
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Filing: 1985-09-30
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Granted: 1989-02-21
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Expires: 2005-09-30
Abstract
Abstract Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon whose amide groups have been partially solvolyzed. Solvolysis of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by addition of water. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample.
Inventors
- 👤 Robert J. Carrico
- 👤 Robert P. Hatch
- 👤 William L. Patterson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
45 words
A method for immobilizing a nucleic acid consisting essentially of the step of contacting the nucleic acid with a solid support consisting essentially of nylon whose amide groups have been partially solvolyzed whereby said nucleic acid becomes bound to the solid support by noncovalent bonds.
Claim 2
Independent Claim
45 words
A method for immobilizing a nucleic acid consisting essentially of the step of contacting the nucleic acid with a solid support consisting essentially of nylon whose amide groups have been partially solvolyzed whereby said nucleic acid becomes bound to the solid support by noncovalent bonds.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein the nylon is treated with an alkylating agent under anhydrous conditions and thereafter contacted with water.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein the activating agent is a trialkyloxonium salt.
Claim 5
Dependent Claim
49 words
The method of claim 1 wherein the nucleic acid to be immobilized has a predetermined base sequence and after, such nucleic acid has been bound to the nylon support such support is contacted with a nonspecific nucleic acid to saturate any remaining nucleic acid binding sites on such support.
Claim 6
Dependent Claim
16 words
The method of claim 1 wherein the solid support is in the shape of a bead.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the nucleic acid is DNA.
Claim 8
Dependent Claim
11 words
The method of claim 1 wherein the nucleic acid is RNA.
References Cited (Backward Citations)
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Cited
US4581333A
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US4298685A
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US4618649A
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US4652517A
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US4563417A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-21 | 1992-08-22 | 1993-02-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-21 | 1996-08-22 | 1997-02-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-21 | 2000-08-22 | 2001-02-21 | $7700.00 | Window Closed / Lapsed |