US4667025A
Expired / Lapsed
US
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Oligonucleotide derivatives
Assignee: Wakunaga Pharmaceutical Co Ltd
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Filing: 1985-10-24
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Granted: 1987-05-19
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Expires: 2005-10-24
Abstract
Abstract An oligonucleotide derivative having an amino group protected with an eliminatable group bonded through a phosphate group and a spacer with an appropriate length to the 5'-end of an oligonucleotide protected suitably at the 3'-hydroxyl group and the base moiety of the nucleotide, and an immobilized oligonucleotide derivative having a Sepharose carrier bonded to the amino group in place of said protective group are disclosed. Methods for production of these derivatives are also disclosed.
Inventors
- 👤 Kenichi Miyoshi
- 👤 Toru Fuwa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 05:53
CPC Classifications
C; C07; C07H; C07H21/00; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
75 words
An oligonucleotide derivative of the formula: ##STR8## wherein R o is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is a C 2 -C 6 alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; R 3 is cyanoethyl; B' is the same or different and is selected from the group consisting of N 6 -benzoyladenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; and m is 2.
Claim 2
Dependent Claim
75 words
An oligonucleotide derivative of the formula: ##STR8## wherein R o is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is a C 2 -C 6 alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; R 3 is cyanoethyl; B' is the same or different and is selected from the group consisting of N 6 -benzoyladenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; and m is 2.
Claim 3
Dependent Claim
77 words
An oligonucleotide of the formula: ##STR9## wherein R o is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is a C 2 -C 6 alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; B' is the same or different and is selected from the group consisting of N 6 -benzoyladenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; R 4 is polystyrene; m is 2; and n is 1-12.
Claim 4
Dependent Claim
77 words
An oligonucleotide of the formula: ##STR9## wherein R o is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is a C 2 -C 6 alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; B' is the same or different and is selected from the group consisting of N 6 -benzoyladenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; R 4 is polystyrene; m is 2; and n is 1-12.
Claim 5
Dependent Claim
42 words
An oligonucleotide of the formula: ##STR10## wherein R 1 is a C 2 -C 6 alkylene; B is the same or different and is selected from the group consisting of adenin-9-yl, guanin-9-yl, cytosin-1-yl and thymin-1-yl; m is 2; and n is 1-12.
Claim 6
Dependent Claim
42 words
An oligonucleotide of the formula: ##STR10## wherein R 1 is a C 2 -C 6 alkylene; B is the same or different and is selected from the group consisting of adenin-9-yl, guanin-9-yl, cytosin-1-yl and thymin-1-yl; m is 2; and n is 1-12.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-05-18 | 1990-11-17 | 1991-05-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-18 | 1994-11-17 | 1995-05-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-18 | 1998-11-17 | 1999-05-19 | $7700.00 | Window Closed / Lapsed |