EP0281390A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Polycationic supports for nucleic acid purification, separation and hybridization
Assignee: Gen Probe Inc
•
Filing: 1988-03-02
•
Granted: —
•
Expires: 2008-03-02
Abstract
Abstract Described herein is the use of polycationic solid supports in the purification of nucleic acids from solutions containing contaminants. The nucleic acids non-covalently bind to the support without signficant binding of contaminants permitting their separation from the contaminants. The bound nucleic acids can be recovered from the support. Also described is the use of the supports as a means to separate polynucleotides and hybrids thereof with a nucleotide probe from unhybridized probe. Assays for target nucleotide sequences are described which employ this separation procedure.
Inventors
- 👤 J. Arnold Lyle Jr.
- 👤 Norman C. Nelson
- 👤 Mark A. Reynolds
- 👤 Alexander A. Waldrop Iii
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 09:14
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C07; C07H; C07H21/00; C07H1/00; C07H1/06; C12Q1/68; C12Q1/6806; C12Q1/6813; C12Q1/6834; C12Q1/6876; C12Q1/6888; C12Q1/689
Claims Analysis
Total Claims: 120
Claim 1
Independent Claim
48 words
A method for purifying nucleotide multimers in a solution containing contaminants comprising the steps: a. contacting the solution with a polycationic solid support to non-covalently bind the multimer to the support without significant binding of the contaminants and; b. segregating the support and bound multimers from the solution.
Claim 2
Independent Claim
48 words
A method for purifying nucleotide multimers in a solution containing contaminants comprising the steps: a. contacting the solution with a polycationic solid support to non-covalently bind the multimer to the support without significant binding of the contaminants and; b. segregating the support and bound multimers from the solution.
Claim 3
Dependent Claim
15 words
A method according to Claim 1 wherein the support is treated to recover the multimer.
Claim 4
Dependent Claim
31 words
A method according to claim 2 wherein the support is treated with an elution solution to desorb the multimers followed by separation of the elution solution and solid support then separated.
Claim 5
Dependent Claim
26 words
A method according to claim 3 wherein the elution solution is a member of the group consisting of phosphate, pyrophosphate, tripolyphosphate, phytic acid and 50% formamide.
Claim 6
Dependent Claim
15 words
A method according to Claim 3 wherein the multimers are recovered from the elution solution.
Claim 7
Dependent Claim
15 words
A method according to Claim 4 wherein the multimers are recovered from the elution solution.
Claim 8
Independent Claim
25 words
A method according to claims 1, 2, 3, 4, 5, or 6 wherein the solid support is washed after separation from the solution containing contaminants.
Claim 9
Dependent Claim
18 words
A method according to Claim 7 wherein the solution containing the multimers and contaminants is a separation solution.
Claim 10
Independent Claim
17 words
A method according to Claims 1, 2, 3, 4, 5, or 6 wherein the multimers are polynucleotides.
Claim 11
Dependent Claim
17 words
A method according to Claim 9 wherein the contaminants include smaller nucleotide multimers than the bound polynucleotides.
Claim 12
Independent Claim
20 words
A method according to Claims 1, 2, 3, 4, 5 or 6 wherein the solid support is comprised of particles.
Claim 13
Dependent Claim
16 words
A method according to Claim 11 wherein the solid support is comprised of micron sized particles.
Claim 14
Independent Claim
20 words
A method according to claims 1, 2, 3, 4, 5 or 6 wherein the solid support is comprised of fibers.
Claim 15
Independent Claim
19 words
A method according to claims 1, 2, 3, 4, 5 or 6 wherein the solid support is a membrane.
Claim 16
Dependent Claim
26 words
A method according to Claim 12 wherein the solid support is comprised of magnetically responsive particles and segregation is facilitated by exposure to a magnetic field.
Claim 17
Dependent Claim
28 words
A method according to claim 13 wherein the solid support is comprised of micron sized magnetically responsive fibers and segretation is facilitated by exposure to a magnetic field.
Claim 18
Dependent Claim
23 words
A method according to claim 14 wherein the said membrane is magnetically responsive and segregation is facilitated by exposure to a magnetic field.
Claim 19
Independent Claim
26 words
A method according to Claims 1, 2, 3, 4, 5 or 6 wherein the cationic charge is imparted by alkyl or aryl amines, guanidines or imines.
Claim 20
Dependent Claim
22 words
A method according to Claim 18 wherein the solid support is a metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol, or polyaminoacid.
Claim 21
Dependent Claim
90 words
An assay for detecting a target nucleotide sequence in a test solution comprising the steps: a. contacting a labelled nucleotide probe for the target sequence with said test solution under hybridizing conditions to form a hybrid between said nucleotide probe and said target in the test solution; b. contacting a polycationic solid support with said test solution to effectuate formation of a bound phase and a free phase; c. detecting said bound phase or said free phase as a qualitative or quantitative measure of said target in the test solution.
Claim 22
Dependent Claim
90 words
An assay for detecting a target nucleotide sequence in a test solution comprising the steps: a. contacting a labelled nucleotide probe for the target sequence with said test solution under hybridizing conditions to form a hybrid between said nucleotide probe and said target in the test solution; b. contacting a polycationic solid support with said test solution to effectuate formation of a bound phase and a free phase; c. detecting said bound phase or said free phase as a qualitative or quantitative measure of said target in the test solution.
Claim 23
Dependent Claim
112 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. adding to a solution containing polynucleotide suspected of containing the target sequence a nucleotide probe for the target sequence, under hybridizing conditions to form a hybrid between the probe and target sequence if present; b. combining the polynucleotides, including any hybrids with the probe and unhybridized probe in a solution with a polycationic solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. detecting the presence of any unbound probe or probe bound to the solid support as a quantitative or qualitative measure of the target polynucleotide sequence in said test solution.
Claim 24
Dependent Claim
112 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. adding to a solution containing polynucleotide suspected of containing the target sequence a nucleotide probe for the target sequence, under hybridizing conditions to form a hybrid between the probe and target sequence if present; b. combining the polynucleotides, including any hybrids with the probe and unhybridized probe in a solution with a polycationic solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. detecting the presence of any unbound probe or probe bound to the solid support as a quantitative or qualitative measure of the target polynucleotide sequence in said test solution.
Claim 25
Dependent Claim
140 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. adding to a solution containing polynucleotide suspected of containing the target sequence a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides being assayed, under hybridizing conditions to form a hybrid between the probe and target sequence if present; b. combining the polynucleotides, including any hybrids with the probe and unhybridized probe in a solution with a polycationic solid support to non-covalently bind the polynucleotides and hybrids with the probe without significant binding of the unhybridized probe; c. segregating the solid support from the solution containing unhybridized probe; and d. detecting the presence of any probe bound to the solid support as an indication of the presence and/or amount of target sequence in polynucleotides bound to the support.
Claim 26
Dependent Claim
140 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. adding to a solution containing polynucleotide suspected of containing the target sequence a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides being assayed, under hybridizing conditions to form a hybrid between the probe and target sequence if present; b. combining the polynucleotides, including any hybrids with the probe and unhybridized probe in a solution with a polycationic solid support to non-covalently bind the polynucleotides and hybrids with the probe without significant binding of the unhybridized probe; c. segregating the solid support from the solution containing unhybridized probe; and d. detecting the presence of any probe bound to the solid support as an indication of the presence and/or amount of target sequence in polynucleotides bound to the support.
Claim 27
Dependent Claim
131 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. adding to a solution containing polynucleotide suspected of containing the target sequence a nucleotide probe for the target sequence, under hybridizing conditions to form a hybrid between the probe and target sequence if present; b. combining the polynucleotides, including any hybrids with the probe and unhybridized probe in a solution with a polycationic solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. eluting the hybrid, the labelled probe or the label from the solid support using an elution solution; and e. detecting the presence of any unbound probe or probe bound to the solid support as a quantitative or qualitative measure of the target polynucleotide sequence in said test solution.
Claim 28
Dependent Claim
131 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. adding to a solution containing polynucleotide suspected of containing the target sequence a nucleotide probe for the target sequence, under hybridizing conditions to form a hybrid between the probe and target sequence if present; b. combining the polynucleotides, including any hybrids with the probe and unhybridized probe in a solution with a polycationic solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. eluting the hybrid, the labelled probe or the label from the solid support using an elution solution; and e. detecting the presence of any unbound probe or probe bound to the solid support as a quantitative or qualitative measure of the target polynucleotide sequence in said test solution.
Claim 29
Dependent Claim
27 words
A method according to claim 23 wherein the hybrid elution solution is a member of the group consisting of 50% formamide, phosphate, pyrophosphate, tripolyphosphate and phytic acid.
Claim 30
Dependent Claim
22 words
A method according to Claim 22 wherein the polynucleotides being probed are at least about 3 times larger multimers than the probe.
Claim 31
Dependent Claim
22 words
A method according to Claim 22 wherein the polynucleotides being probed are at least about 5 times larger multimers than the probe.
Claim 32
Dependent Claim
38 words
A method according to Claim 20, 21, 22 or 23 wherein the probe is an analogue of DNA or RNA having a lower negative charge in the phosphate backbone than the phosphate diesters of native DNA or RNA.
Claim 33
Dependent Claim
13 words
A method according to Claim 27 wherein the analogue is a methyl phosphonate.
Claim 34
Independent Claim
26 words
A method according to Claims 20, 21, 22 or 23, wherein the segregation of the solid support comprises removing it from the solution containing unhybridized probe.
Claim 35
Independent Claim
55 words
A method according to claims 20, 21, 22 or 23 wherein the probe is an analogue of DNA or RNA having a lower negative charge in the phosphate backbone than the phosphate diesters of native DNA or RNA and wherein the segregation of the solid support comprises removing it from the solution containing unhybridized probe.
Claim 36
Independent Claim
66 words
A method according to claims 20, 21, 22 or 23 wherein the probe is an analogue of DNA or RNA having a lower negative charge in the phosphate backbone than the phosphate diesters of native DNA or RNA and wherein the segregation of the solid support comprises removing it from the solution containing unhybridized probe and wherein the probe has a label which permits its detection.
Claim 37
Dependent Claim
18 words
A method according to Claim 30 wherein the solid support is washed prior to detection of the probe.
Claim 38
Dependent Claim
18 words
A method according to Claim 31 wherein the solid support is washed prior to detection of the probe.
Claim 39
Independent Claim
20 words
A method according to Claims 20, 21, 22 or 23 wherein the probe has a label which permits its detection.
Claim 40
Independent Claim
18 words
A method according to Claims 20, 21, 22 or 23 wherein the solid support is comprised of particles.
Claim 41
Dependent Claim
16 words
A method according to Claim 35 wherein the solid support is comprised of micron sized particles.
Claim 42
Dependent Claim
18 words
A method according to claim 20, 21, 22 or 23 wherein the solid support is comprised of fibers.
Claim 43
Dependent Claim
17 words
A method according to claim 20, 21, 22 or 23 wherein the solid support is a membrane.
Claim 44
Dependent Claim
28 words
A method according to Claim 36 wherein the solid support is comprised of magnetically responsive particles and segregation is obtained by exposing said particles to a magnetic field.
Claim 45
Dependent Claim
28 words
A method according to claim 37 wherein the solid support is comprised of magnetically responsive fibers and segregation is obtained by exposing said fibers to a magnetic field.
Claim 46
Dependent Claim
27 words
A method according to claim 38 wherein the solid support is a magnetically responsive membrane and segregation is obtained by exposing said membrane to a magnetic field.
Claim 47
Independent Claim
24 words
A method according to Claims 20, 21, 22 or 23 wherein the cationic charge is imparted by alkyl or aryl amines, guanidines or imines.
Claim 48
Dependent Claim
22 words
A method according to Claim 42 wherein the segregation of the solid support comprises removing it from the solution containing unhybridized probe.
Claim 49
Dependent Claim
14 words
A method according to Claim 43 wherein the solid support comprises micron sized particles.
Claim 50
Dependent Claim
12 words
A method according to claim 43 wherein the solid support comprises fibers.
Claim 51
Dependent Claim
13 words
A method according to claim 43 wherein the solid support is a membrane.
Claim 52
Dependent Claim
26 words
A method according to Claim 44 wherein the particles are an magnetically responsive and the segregation is obtained by exposing the particles to a magnetic field.
Claim 53
Dependent Claim
26 words
A method according to Claim 45 wherein the fibers are an magnetically responsive and the segregation is obtained by exposing the particles to a magnetic field.
Claim 54
Dependent Claim
25 words
A method according to Claim 46 wherein the membrane is magnetically responsive and the segregation is obtained by exposing the particles to a magnetic field.
Claim 55
Dependent Claim
20 words
A method according to Claim 47 wherein the particles are metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 56
Dependent Claim
20 words
A method according to Claim 48 wherein the fibers are metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 57
Dependent Claim
20 words
A method according to Claim 49 wherein the membrane is metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 58
Dependent Claim
128 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. contacting a solution containing polynucleotides suspected of containing the target sequence with a polycationic solid support to non-covalently bind the polynucleotides to the solid support; b. contacting the solid support and bound polynucleotides with a solution containing a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides being assayed, under hybridizing conditions to form a hybrid between the probe and target sequence, if present, without significant binding of the unhybridized probe to the solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. detecting the presence of the hybrid, either qualitatively or quantitatively, as a measure of target sequence in polynucleotides.
Claim 59
Dependent Claim
128 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. contacting a solution containing polynucleotides suspected of containing the target sequence with a polycationic solid support to non-covalently bind the polynucleotides to the solid support; b. contacting the solid support and bound polynucleotides with a solution containing a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides being assayed, under hybridizing conditions to form a hybrid between the probe and target sequence, if present, without significant binding of the unhybridized probe to the solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. detecting the presence of the hybrid, either qualitatively or quantitatively, as a measure of target sequence in polynucleotides.
Claim 60
Dependent Claim
146 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. contacting a solution containing polynucleotides suspected of containing the target sequence with a polycationic solid support to non-covalently bind the polynucleotides to the solid support; b. contacting the solid support and bound polynucleotides with a solution containing a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides being assayed, under hybridizing conditions to form a hybrid between the probe and target sequence, if present, without significant binding of the unhybridized probe to the solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. eluting the hybrid, the labelled probe or the label from the solid support using an elution solution; e. detecting the presence of the hybrid, either quantitatively or qualitatively, as a measure of target sequence in polynucleotides.
Claim 61
Dependent Claim
146 words
An assay for detecting a target nucleotide sequence in a polynucleotide comprising the steps: a. contacting a solution containing polynucleotides suspected of containing the target sequence with a polycationic solid support to non-covalently bind the polynucleotides to the solid support; b. contacting the solid support and bound polynucleotides with a solution containing a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides being assayed, under hybridizing conditions to form a hybrid between the probe and target sequence, if present, without significant binding of the unhybridized probe to the solid support; c. segregating the solid support from the solution containing unhybridized probe; and d. eluting the hybrid, the labelled probe or the label from the solid support using an elution solution; e. detecting the presence of the hybrid, either quantitatively or qualitatively, as a measure of target sequence in polynucleotides.
Claim 62
Dependent Claim
24 words
A method according to Claim 53 or 54 wherein the polynucleotides being probed are at least about 3 times larger multimer than the probe.
Claim 63
Dependent Claim
24 words
A method according to Claim 53 or 54 wherein the polynucleotides being probed are at least about 5 times larger multimers than the probe.
Claim 64
Dependent Claim
36 words
A method according to Claim 53 or 54 wherein the probe is an analogue of DNA or RNA having a lower negative charge in the phosphate backbone than the phosphate diesters of native DNA or RNA.
Claim 65
Dependent Claim
13 words
A method according to Claim 53 wherein the analogue is a methyl phosphonate.
Claim 66
Dependent Claim
13 words
A method according to Claim 54 wherein the analogue is a methyl phosphonate.
Claim 67
Dependent Claim
22 words
A method according to Claim 58 wherein the segregation of the solid support comprises removing it from the solution containing unhybridized probe.
Claim 68
Independent Claim
30 words
A method according to Claims 59 wherein the segregation of the solid support comprises removing it from the solution containing unhybridized probe and wherein the analogue is a methyl phosphonate.
Claim 69
Dependent Claim
18 words
A method according to Claim 60 wherein the solid support is washed prior to detection of the probe.
Claim 70
Dependent Claim
18 words
A method according to claim 61 wherein the solid support is washed prior to detection of the probe.
Claim 71
Independent Claim
18 words
A method according to Claims 53 or 54 wherein the probe has a label which permits its detection.
Claim 72
Independent Claim
16 words
A method according to Claims 53 or 54 wherein the solid support is comprised of particles.
Claim 73
Dependent Claim
16 words
A method according to Claim 65 wherein the solid support is comprised of micron sized particles.
Claim 74
Independent Claim
16 words
A method according to claims 53 or 54 wherein the solid support is comprised of fibers.
Claim 75
Independent Claim
15 words
A method according to claims 53 or 54 wherein the solid support is a membrane.
Claim 76
Dependent Claim
28 words
A method according to Claim 66 wherein the solid support is comprised of magnetically responsive particles and segregation is obtained by exposing the particles to a magnetic field.
Claim 77
Dependent Claim
28 words
A method according to Claim 67 wherein the solid support is comprised of magnetically responsive particles and segregation is obtained by exposing the particles to a magnetic field.
Claim 78
Dependent Claim
28 words
A method according to Claim 68 wherein the solid support is comprised of magnetically responsive particles and segregation is obtained by exposing the particles to a magnetic field.
Claim 79
Independent Claim
22 words
A method according to Claims 53 or 54 wherein the cationic charge is imparted by alkyl or aryl amines, guanidines or imines.
Claim 80
Dependent Claim
22 words
A method according to Claim 72 wherein the segregation of the solid support comprises removing it from the solution containing unhybridized probe.
Claim 81
Dependent Claim
14 words
A method according to Claim 72 wherein the solid support comprises micron sized particles.
Claim 82
Dependent Claim
12 words
A method according to claim 72 wherein the solid support comprises fibers.
Claim 83
Dependent Claim
13 words
A method according to claim 72 wherein the solid support is a membrane.
Claim 84
Dependent Claim
25 words
A method according to claim 74 wherein the particles are magnetically responsive and the segragation is obtained by exposing the particles to a magnetic field.
Claim 85
Dependent Claim
25 words
A method according to Claim 75 wherein the fibers are magnetically responsive and the segregation is obtained by exposing the fibers to a magnetic field.
Claim 86
Dependent Claim
25 words
A method according to Claim 76 wherein the membrane is magnetically responsive and the segregation is obtained by exposing the membrane to a magnetic field.
Claim 87
Dependent Claim
20 words
A method according to Claim 77 wherein the particles are metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 88
Dependent Claim
20 words
A method according to Claim 78 wherein the fibers are metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 89
Dependent Claim
20 words
A method according to Claim 79 wherein the membrane is metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 90
Dependent Claim
44 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the target sequence comprising: a. a nucleotide probe for the target sequence; and b. a polycationic support capable of separating polynucleotides according to their charge or length.
Claim 91
Dependent Claim
44 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the target sequence comprising: a. a nucleotide probe for the target sequence; and b. a polycationic support capable of separating polynucleotides according to their charge or length.
Claim 92
Dependent Claim
49 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the target sequence comprising: a. a nucleotide probe for the target sequence; b. a polycationic support capable of separating polynucleotides according to their charge or length; and c. a hybrid elution solution.
Claim 93
Dependent Claim
49 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the target sequence comprising: a. a nucleotide probe for the target sequence; b. a polycationic support capable of separating polynucleotides according to their charge or length; and c. a hybrid elution solution.
Claim 94
Dependent Claim
65 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the target sequence comprising: a. a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides to be assayed; and b. a polycationic support capable of non-covalently binding the polynucleotides and hybrids thereof with the probe without significantly binding the unhybridized probe.
Claim 95
Dependent Claim
65 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the target sequence comprising: a. a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides to be assayed; and b. a polycationic support capable of non-covalently binding the polynucleotides and hybrids thereof with the probe without significantly binding the unhybridized probe.
Claim 96
Dependent Claim
70 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the sequence comprising: a. a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides to be assayed; and b. a polycationic support capable of non-covalently binding the polynucleotides and hybrids thereof with the probe without significantly binding the unhybridized probe; and c. a hybrid elution solution.
Claim 97
Dependent Claim
70 words
A kit for assaying for a target nucleotide sequence in a solution containing polynucleotides suspected of containing the sequence comprising: a. a nucleotide probe for the target sequence, the probe being a smaller nucleotide multimer than the polynucleotides to be assayed; and b. a polycationic support capable of non-covalently binding the polynucleotides and hybrids thereof with the probe without significantly binding the unhybridized probe; and c. a hybrid elution solution.
Claim 98
Dependent Claim
29 words
A kit according to Claim 84 or 86 wherein the hybrid elution solution is a member of the group consisting of 50% formamide, phosphate, pyrophosphate, tripolyphosphate and phytic acid.
Claim 99
Dependent Claim
27 words
A kit according to Claim 85 wherein the probe is selected so that the polynucleotides being probed are at least about 3 times larger than the probe.
Claim 100
Dependent Claim
21 words
A kit according to Claim 85 wherein the polynucleotides being probed are at least about 5 times larger than the probe.
Claim 101
Dependent Claim
40 words
A kit according to Claim 83 or 84 or 85 or 86 wherein the probe is an analogue of DNA or RNA having a lower negative charge in the phosphate backbone than the phosphate diesters of native DNA or RNA.
Claim 102
Dependent Claim
13 words
A kit according to Claim 90 wherein the analogue is a methyl phosphonate.
Claim 103
Dependent Claim
22 words
A kit according to Claims 83 or 84 or 85 or 86 wherein the probe has a label to permit its detection.
Claim 104
Dependent Claim
20 words
A kit according to Claim 83 or 84 or 85 or 86 wherein the solid support is comprised of particles.
Claim 105
Dependent Claim
16 words
A kit according to Claim 93 wherein the solid support is comprised of micron sized particles.
Claim 106
Dependent Claim
20 words
A kit according to Claim 83 or 84 or 85 or 86 wherein the solid support is comprised of fibers.
Claim 107
Dependent Claim
19 words
A kit according to Claim 83 or 84 or 84 or 86 wherein the solid support is a membrane.
Claim 108
Dependent Claim
12 words
A kit according to Claim 94 wherein the particles are magnetically responsive.
Claim 109
Dependent Claim
12 words
A kit according to claim 95 wherein the fibers are magnetically responsive.
Claim 110
Dependent Claim
12 words
A kit according to claim 96 wherein the membrane is magnetically responsive.
Claim 111
Dependent Claim
26 words
A kit according to Claims 83 or 84 or 85 or 86 wherein the cationic charge is imparted by alkyl or aryl amines, guanidines or imines.
Claim 112
Dependent Claim
14 words
A kit according to Claim 100 wherein the solid support is comprised of particles.
Claim 113
Dependent Claim
14 words
A kit according to claim 100 wherein the solid support is comprised of fibers.
Claim 114
Dependent Claim
13 words
A kit according to claim 100 wherein the solid support is a membrane.
Claim 115
Dependent Claim
12 words
A kit according to Claim 101 wherein the particles are magnetically responsive.
Claim 116
Dependent Claim
12 words
A kit according to Claim 102 wherein the particles are magnetically responsive.
Claim 117
Dependent Claim
12 words
A kit according to Claim 103 wherein the particles are magnetically responsive.
Claim 118
Dependent Claim
21 words
A kit according to Claim 103 wherein the particles are a metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 119
Dependent Claim
21 words
A kit according to Claim 104 wherein the fibers are a metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
Claim 120
Dependent Claim
21 words
A kit according to Claim 105 wherein the membrane is a metal oxide, glass, polyamide, polyester, polyolefin, polysaccharide, polyglycol or polyaminoacid.
References Cited (Backward Citations)
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