US4789737A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oligonucleotide derivatives and production thereof
Assignee: Wakunaga Pharmaceutical Co Ltd
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Filing: 1987-02-20
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Granted: 1988-12-06
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Expires: 2007-02-20
Abstract
Abstract An oligonucleotide derivative having an amino group protected with an eliminatable group bonded through a phosphate group and a spacer with an appropriate length to the 5'-end of an oligonucleotide protected suitably at the 3'-hydroxyl group and the base moiety of the nucleotide, and an immobilized oligonucleotide derivative having a Sepharose carrier bonded to the amino group in place of said protective group are disclosed. Methods for production of these derivatives are also disclosed.
Inventors
- 👤 Kenichi Miyoshi
- 👤 Toru Fuwa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:46
CPC Classifications
C; C07; C07H; C07H21/00; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
77 words
An oligonucleotide derivative of the formula: ##STR29## wherein R° is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is C 2-20 straight or branched alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; R 3 is cyanoethyl; B' may be the same or different and is selected from the group consisting of N 6 -benzolyadenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; and m is 2 to 6.
Claim 2
Dependent Claim
77 words
An oligonucleotide derivative of the formula: ##STR29## wherein R° is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is C 2-20 straight or branched alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; R 3 is cyanoethyl; B' may be the same or different and is selected from the group consisting of N 6 -benzolyadenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; and m is 2 to 6.
Claim 3
Dependent Claim
88 words
An oligonucleotide of the formula: ##STR30## wherein R° is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is C 2-20 straight or branched alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; B' may be the same or different and is selected from the group consisting of N 6 -benzolyadenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; and R 4 is a polymeric carrier comprising polystyrene or polyacrylamide; m is 2 to 6; and n is 0 to 40.
Claim 4
Dependent Claim
88 words
An oligonucleotide of the formula: ##STR30## wherein R° is selected from the group consisting of o-chlorophenyl and p-chlorophenyl; R 1 is C 2-20 straight or branched alkylene; R 2 is selected from the group consisting of trifluoroacetyl and o-nitrophenyl sulphenyl; B' may be the same or different and is selected from the group consisting of N 6 -benzolyadenin-9-yl, N 4 -benzoylcytosin-1-yl, N 2 -isobutyrylguanin-9-yl, and thymin-1-yl; and R 4 is a polymeric carrier comprising polystyrene or polyacrylamide; m is 2 to 6; and n is 0 to 40.
Claim 5
Dependent Claim
46 words
An oligonucleotide of the formula: ##STR31## wherein R 1 is C 2-20 straight or branched alkylene; B is the same or different and is selected from the group consisting of adenin-9-yl, guanin-9-yl, cytosin-1-yl and thymin-1-yl; m is 2 to 6; and n is 0 to 40.
Claim 6
Dependent Claim
46 words
An oligonucleotide of the formula: ##STR31## wherein R 1 is C 2-20 straight or branched alkylene; B is the same or different and is selected from the group consisting of adenin-9-yl, guanin-9-yl, cytosin-1-yl and thymin-1-yl; m is 2 to 6; and n is 0 to 40.
References Cited (Backward Citations)
US4667025A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-12-06 | 1992-06-06 | 1992-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-12-06 | 1996-06-06 | 1996-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-12-06 | 2000-06-06 | 2000-12-06 | $7700.00 | Window Closed / Lapsed |