US5264104A
Expired / Lapsed
US
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Enzyme electrodes
Assignee: Therasense Inc
•
Filing: 1992-05-08
•
Granted: 1993-11-23
•
Expires: 2012-05-08
Abstract
Abstract An improved enzyme electrode includes a three-dimensional redox polymer network having redox enzymes bound thereto, and also having a protein excluding polymeric coating.
Inventors
- 👤 Brian A. Gregg
- 👤 Adam Heller
- 👤 Wolfgang Kerner
- 👤 Michael V. Pishko
- 👤 Ioanis Katakis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 08:04
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
49 words
An enzyme electrode comprising: an electrode having a testing surface; and a transducing film covering the testing surface of the electrode which film is formed by curing on the electrode surface a solution comprising a redox polymer and a hydroxylated di-, tri- or poly- triaziridine as a crosslinking agent.
Claim 2
Dependent Claim
49 words
An enzyme electrode comprising: an electrode having a testing surface; and a transducing film covering the testing surface of the electrode which film is formed by curing on the electrode surface a solution comprising a redox polymer and a hydroxylated di-, tri- or poly- triaziridine as a crosslinking agent.
Claim 3
Dependent Claim
23 words
The electrode of claim 1, wherein the transducing film comprises a cross-linked redox polymer network to which a redox enzyme is chemically bound.
Claim 4
Dependent Claim
15 words
The electrode of claim 2, wherein the hydroxylated di- or poly- triaziridine is pentaerythritol-tris-(B-(N-aziridinyl propionate).
Claim 5
Dependent Claim
62 words
An enzyme electrode comprising: an electrode having a testing surface; and a transducing film covering the testing surface which film comprises a cross-linked redox polymer network to which a redox enzyme is chemically bound, wherein the three-dimensional redox polymer network is formed by curing on the electrode surface a solution comprising a redox polymer derived of poly(vinyl imidazole), and a redox enzyme.
Claim 6
Dependent Claim
62 words
An enzyme electrode comprising: an electrode having a testing surface; and a transducing film covering the testing surface which film comprises a cross-linked redox polymer network to which a redox enzyme is chemically bound, wherein the three-dimensional redox polymer network is formed by curing on the electrode surface a solution comprising a redox polymer derived of poly(vinyl imidazole), and a redox enzyme.
Claim 7
Dependent Claim
20 words
The enzyme electrode of claim 4, wherein the redox polymer is poly(vinyl imidazole) complexed with di- or tri-valent osmium ions.
Claim 8
Dependent Claim
20 words
The enzyme electrode of claim 5, wherein the redox polymer is poly(N-vinyl imidazole) complexed with [osmium bis (2,2'-bipyridine)Cl] +/+2 .
Claim 9
Dependent Claim
18 words
The enzyme electrode of claim 5, wherein the curing solution further comprises a di-, tri-, or poly-aziridine crosslinker.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-22 | 1997-05-24 | 1997-11-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-22 | 2001-05-24 | 2001-11-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-22 | 2005-05-24 | 2005-11-23 | $7700.00 | Window Closed / Lapsed |