EP0184909A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Bioelectrochemical assay and apparatus
Assignee: Genetics International Inc
•
Filing: 1985-11-13
•
Granted: —
•
Expires: 2005-11-13
Abstract
Abstract Salts of TCNQ (7,7,8,8-tetracyano-p-quinodimethane such as the copper (dipyridylamine), tetrathiafulvalene (TTF), ferrocene, triethylamine and quinoline salts are used in the fabrication of electrodes for direct electrical determination of the progress of flavoprotein enzyme reactions, the enzyme preferably being associated at the electrode surface. Choline oxidase and pyruvate oxidase for phosphate assay, are preferred enzymes and the salt TTF + TCNQ - is the preferred salt. TTF + TCNQ - also permits direct electrical coupling |NAD/NADH to the electrode.
Inventors
- 👤 Wyndham John Albery
- 👤 Nigel Philip Bartlett
- 👤 Derek Harry Craston
- 👤 Mark Bycroft
- 👤 Christopher Peter Jones
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 15:15
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
44 words
An electrode for use in assay system which electrode exhibits at an operative surface (a) a material having one-dimensional electrical conduction properties and (b) a flavoprotein enzyme chosen from glucose oxidase, L-aminoacid oxidase. D-aminoacid oxidase, choline oxidase, xanthine oxidase, monoamine oxidase and pyruvate oxidase.
Claim 2
Dependent Claim
44 words
An electrode for use in assay system which electrode exhibits at an operative surface (a) a material having one-dimensional electrical conduction properties and (b) a flavoprotein enzyme chosen from glucose oxidase, L-aminoacid oxidase. D-aminoacid oxidase, choline oxidase, xanthine oxidase, monoamine oxidase and pyruvate oxidase.
Claim 3
Dependent Claim
16 words
An electrode as claimed in claim 1, in which the said material is an organic conductor.
Claim 4
Dependent Claim
23 words
An electrode as claimed in claim 1 or 2, in which the said material is a derivative or salt of 7,7,8,8-tetracyano p-quinodimethane TCNQ.
Claim 5
Dependent Claim
27 words
An electrode as claimed in claim 3 in which the TCNQ material further comprises copper (dipyridylamine). tetrathiafulvalene. ferrocene, triethylamine or quinoline in the form of positive ions.
Claim 6
Dependent Claim
22 words
An electrode as claimed in claim 3 in which the enzyme is choline oxidase and the TCNQ salt is the tetrathiafulvalene salt.
Claim 7
Dependent Claim
26 words
An electrode as claimed in claim 3 in which the enzyme is pyruvate oxidase and the TCNQ salt is the tetrathiafulvalene salt or the N-methyl-phenazine salt.
Claim 8
Dependent Claim
66 words
An electrode for use in an assay system which electrode exhibits at an operative surface (a) a material having one-dimensional electrical conduction properties chosen from copper (dipyridylamine). tetrathiafulvalene, ferrocene, triethylamine and quinoline salts of 7,7,8,8-tetracyano p-quinodimethane, (TCNQ) together with (b) an enzyme located at least at the said operative surface of said electrode whereby charge is transferred to said electrode when the enzyme is catalytically active.
Claim 9
Dependent Claim
66 words
An electrode for use in an assay system which electrode exhibits at an operative surface (a) a material having one-dimensional electrical conduction properties chosen from copper (dipyridylamine). tetrathiafulvalene, ferrocene, triethylamine and quinoline salts of 7,7,8,8-tetracyano p-quinodimethane, (TCNQ) together with (b) an enzyme located at least at the said operative surface of said electrode whereby charge is transferred to said electrode when the enzyme is catalytically active.
Claim 10
Dependent Claim
14 words
An electrode as claimed in claim 7 in which the enzyme is a flavoprotein.
Claim 11
Dependent Claim
76 words
An electrode as claimed in any one of the preceding claims 1, 2, 3, 4, 7 or 8, in which a second enzyme is also located at least at the said operative surface of the said electrode, in addition to said first enzyme. said second enzyme being active to convert a second-enzyme substrate to a first-enzyme substrate whereby said first enzyme when catalytically active provides an electrical signal related to the concentration of the second-enzyme substrate.
Claim 12
Dependent Claim
65 words
An electrode as claimed in claim 1, 2, 3 4. 7 or 8 in which a substrate for a second enzyme is located at least at the said operative surface of the said electrode, said substrate being convertible by said second-enzyme to a first-enzyme substrate whereby said first enzyme when catalytically active provides an electrical signal related to the active concentration of the second enzyme.,
Claim 13
Independent Claim
51 words
A method of assay in which there is contacted with a liquid vehicle containing a component to be selectively detected, measured or monitored an electrode as claimed in any one of claims 1 and 8, wherein the enzyme on said electrode is specific to catalyse a reaction of the said component.
Claim 14
Independent Claim
61 words
A method of assay in which there is contacted with a liquid vehicle containing flavoprotein enzyme, chosen from glucose oxidase, L-aminoacid oxidase. D-aminoacid oxidase, choline oxidase, xanthine oxidase, monoamine oxidase and pyruvate oxidase, together with a component selectively catalysed thereby to be detected, measured or monitored, an electrode which exhibits at an operative surface a material having one-dimensional electrical conduction properties.
Claim 15
Independent Claim
61 words
A method of assay in which there is contacted with a liquid vehicle containing flavoprotein enzyme, chosen from glucose oxidase, L-aminoacid oxidase. D-aminoacid oxidase, choline oxidase, xanthine oxidase, monoamine oxidase and pyruvate oxidase, together with a component selectively catalysed thereby to be detected, measured or monitored, an electrode which exhibits at an operative surface a material having one-dimensional electrical conduction properties.
Claim 16
Dependent Claim
19 words
A method as claiaed in claim 12 in which the said one-dimensionally conductive material is a salt of TCNQ.
Claim 17
Dependent Claim
17 words
A method as claimed in claim 13 in which the salt is TTF + TCNQ - .
Claim 18
Independent Claim
51 words
A method of assay in which there is contacted with a liquid vehicle containing a flavoprotein enzyme and a component selectively catalysed thereby to be detected, measured or monitored, an electrode which exhibits at an operative surface a TCNQ salt chosen from the copper (dipyridylamine). tetrathiafulvalene, ferrocene. triethylamine and quinoline salts.
Claim 19
Independent Claim
51 words
A method of assay in which there is contacted with a liquid vehicle containing a flavoprotein enzyme and a component selectively catalysed thereby to be detected, measured or monitored, an electrode which exhibits at an operative surface a TCNQ salt chosen from the copper (dipyridylamine). tetrathiafulvalene, ferrocene. triethylamine and quinoline salts.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.