EP0241309A2
Expired / Lapsed
EP
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Measurement of electroactive species in solution
Assignee: Medisense Inc
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Filing: 1987-04-10
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Granted: —
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Expires: 2007-04-10
Abstract
Abstract In order to improve the sensitivity of electrochemical assays based on an electrochemical system capable of generating a current, where the system comprises as components an enzyme, a substrate, and a mediator, with the mediator mediating transfer of electrons between the enzyme and an electrode while the enzyme is catalysing a reaction of the substrate, the mediator is indirectly linked with the electrode by a compound capable of accumulating electrical charge for measurement after a delay period.
Inventors
- 👤 Nicholas John Walton
- 👤 Gill Alison Chambers
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 17:49
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
74 words
An electrochemical assay based on an electrochemical system capable of generating a current, potential or charge, the system comprising as components an enzyme, a substrate, and a mediator, with the mediator mediating transfer of electrons between the enzyme and an electrode while the enzyme is catalysing a reaction of the substrate, wherein the mediator is indirectly linked with the electrode by a compound capable of accumulating electrical charge for measurement after a delay period.
Claim 2
Dependent Claim
74 words
An electrochemical assay based on an electrochemical system capable of generating a current, potential or charge, the system comprising as components an enzyme, a substrate, and a mediator, with the mediator mediating transfer of electrons between the enzyme and an electrode while the enzyme is catalysing a reaction of the substrate, wherein the mediator is indirectly linked with the electrode by a compound capable of accumulating electrical charge for measurement after a delay period.
Claim 3
Dependent Claim
21 words
The assay of claim 1, wherein said electrical charge is accumulated during an incubation period, and said electrode is then interrogated.
Claim 4
Dependent Claim
30 words
The assay of claim 2, wherein for said interrogation, said electrode is returned potentiostatically or galvanostatically to its original potential with integration in either case of the resulting current-time transient.
Claim 5
Dependent Claim
20 words
The assay of claim 1, which is a heterogenous assay and said charge accumulator compound is immobilized at said electrode.
Claim 6
Dependent Claim
49 words
The assay of claim 1, which is a homogenous assay comprising admixing said substrate, a first redox reagent as said mediator, a second redox reagent as said charge accumulator compound, and said enzyme; poising said electrode; incubating the system on open circuit; re-poising said electrode; and monitoring the current.
Claim 7
Dependent Claim
9 words
The assay of claim 1 which is an immunoassay.
Claim 8
Dependent Claim
14 words
The assay of claim 6, where said mediator is a label on an antigen.
Claim 9
Dependent Claim
24 words
A dry strip electrode for use in the assay of claim 7 and comprising the enzyme, substrate, mediator-labelled antigen, charge accumulator compound, and antibody.
Claim 10
Dependent Claim
89 words
In an assay of the kind which involves providing an electrochemical system capable of generating a current, the system comprising as components an enzyme, a substrate, and a mediator, with the mediator mediating transfer of electrons between the enzyme and an electrode while the enzyme is catalysing a reaction of the substrate; the improvement which consists of the steps of providing a charge accumulator compound to accumulate a deficit or surfeit of said electrons during an incubation period, and subsequently monitoring the current represented by said deficit or surfeit.
Claim 11
Dependent Claim
89 words
In an assay of the kind which involves providing an electrochemical system capable of generating a current, the system comprising as components an enzyme, a substrate, and a mediator, with the mediator mediating transfer of electrons between the enzyme and an electrode while the enzyme is catalysing a reaction of the substrate; the improvement which consists of the steps of providing a charge accumulator compound to accumulate a deficit or surfeit of said electrons during an incubation period, and subsequently monitoring the current represented by said deficit or surfeit.
References Cited (Backward Citations)
No citation records indexed.
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