US4552840A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme electrode and method for dextran analysis
Assignee: California and Hawaiian Sugar Co
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Filing: 1982-12-02
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Granted: 1985-11-12
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Expires: 2002-12-02
Abstract
Abstract A method for the potentiometric determination of a dextran solution is provided wherein the dextran is enzymatically hydrolyzed to glucose, the glucose is oxidized to form hydrogen peroxide and the hydrogen peroxide is measured utilizing a redox electrode. A novel electrode having a plurality of enzyme impregnated layers is provided for converting the dextran to glucose.
Inventors
- 👤 Richard Riffer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 03:27
CPC Classifications
C; C12; C12M; C12M21/00; C12M21/18; C12M29/00; C12M29/04; C12M41/00; C12M41/30; C12M41/32; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
108 words
An electrode for the potentiometric determination of dextran in a solution comprising a platinum redox electrode, a first cellulose absorbent sheath surrounding the electrode with immobilized glucose oxidase, a second absorbent sheath thereover with immobilized enzymes selected from the group consistinng of α-glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α-glucosidase is selected and when the solution contains starch and an outer absorbent sheath with immobilized dextranase surrounding said means wherein the amounts of glucose oxidase, α-glucosidase or isomaltase and dextranase are sufficient to determine the amount of dextran.
Claim 2
Dependent Claim
108 words
An electrode for the potentiometric determination of dextran in a solution comprising a platinum redox electrode, a first cellulose absorbent sheath surrounding the electrode with immobilized glucose oxidase, a second absorbent sheath thereover with immobilized enzymes selected from the group consistinng of α-glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α-glucosidase is selected and when the solution contains starch and an outer absorbent sheath with immobilized dextranase surrounding said means wherein the amounts of glucose oxidase, α-glucosidase or isomaltase and dextranase are sufficient to determine the amount of dextran.
Claim 3
Dependent Claim
12 words
The electrode of claim 1 wherein said means is a dialysis membrane.
Claim 4
Dependent Claim
9 words
The electrode of claim 1 wherein α-glucosidase is selected.
Claim 5
Dependent Claim
9 words
The electrode of claim 1 wherein isomaltase is selected.
Claim 6
Dependent Claim
16 words
The electrode of claim 1 wherein each sheath is made from a cellulose filter paper sheet.
Claim 7
Dependent Claim
19 words
The electrode of claim 5 wherein each enzyme is immobilized on cellulose by covalent bonding utilizing a bifunctional reagent.
Claim 8
Dependent Claim
12 words
The electrode of claim 6 wherein the bifunctional reagent is sym-triazine trichloride.
Claim 9
Dependent Claim
11 words
The electrode of claim 1 wherein the dextranase is mold dextranase.
Claim 10
Independent Claim
129 words
A method for the potentiometric determination of a dextran in a solution comprising immersing into a sample, an electrode containing a platinum redox electrode, a first cellulose absorbent sheath surrounding the electrode with immobilized glucose oxidase, a second absorbent sheath thereover with immobilized enzymes selected from the group consisting of α-glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α-glucosidase is selected and when the solution contains starch and an outer absorbent sheath with immobilized dextranase surrounding said means wherein the amounts of glucose oxidase, α-glucosidase or isomaltase and dextranase are sufficient to determine the amount of dextran, incubating and measuring the voltage which correlates with the amount of dextran present.
Claim 11
Independent Claim
129 words
A method for the potentiometric determination of a dextran in a solution comprising immersing into a sample, an electrode containing a platinum redox electrode, a first cellulose absorbent sheath surrounding the electrode with immobilized glucose oxidase, a second absorbent sheath thereover with immobilized enzymes selected from the group consisting of α-glucosidase and isomaltase, with the provision that a means for excluding compounds having a molecular weight of 14,000 or greater surrounding said second sheath is included when α-glucosidase is selected and when the solution contains starch and an outer absorbent sheath with immobilized dextranase surrounding said means wherein the amounts of glucose oxidase, α-glucosidase or isomaltase and dextranase are sufficient to determine the amount of dextran, incubating and measuring the voltage which correlates with the amount of dextran present.
Claim 12
Dependent Claim
9 words
The method of claim 9 wherein α-glucosidase is selected.
Claim 13
Dependent Claim
9 words
The method of claim 9 wherein isomaltase is selected.
Claim 14
Dependent Claim
12 words
The method of claim 9 wherein said means is a dialysis membrane.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-11-11 | 1989-05-13 | 1989-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-11-11 | 1993-05-13 | 1993-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-11-11 | 1997-05-13 | 1997-11-12 | $7700.00 | Window Closed / Lapsed |