US3875009A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biological products
Assignee: Colonial Sugar Refining Co Ltd
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Filing: 1973-07-20
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Granted: 1975-04-01
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Expires: 1993-07-20
Abstract
Abstract Method of producing dextranase by culturing a dextranaseproducing strain of the fungus Fusarium fusarioides, for example, Fusarium fusarioides I.M.I. No. 158,405, Dactylium fusarioides A.T.C.C. No. 14167, Fusarium chlamydosporum C.B.S. No. 145.25, Fusarium roseum A.T.C.C. No. 13379, and Fusarium tricinctum C.B.S. No. 261.51, or a mutant or variant of said strain, in a fermentation medium, and recovering the dextranase which accumulates therein.
Inventors
- 👤 Kenneth Trevor Glasziou
- 👤 Charles Roger Slack
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 10:52
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2451; C12N9/2454; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/929
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
35 words
A METHOD OF PRODUCING DEXTRANASE COMPRISING CULTURING A DEXTRANASE-PRODUCING STRAIN OF THE FUNGUS FUSARIUM FUSARI03OIDES, OR A MUTANT OR VARIANT OF SAID DEXTRANASE-PRODUCING STRAIN, IN A FERMENTATION MEDIUM, AND RECOVERING THE DEXTRANASE WHICH ACCUMULATES THEREIN.
Claim 2
Independent Claim
35 words
A METHOD OF PRODUCING DEXTRANASE COMPRISING CULTURING A DEXTRANASE-PRODUCING STRAIN OF THE FUNGUS FUSARIUM FUSARI03OIDES, OR A MUTANT OR VARIANT OF SAID DEXTRANASE-PRODUCING STRAIN, IN A FERMENTATION MEDIUM, AND RECOVERING THE DEXTRANASE WHICH ACCUMULATES THEREIN.
Claim 3
Independent Claim
70 words
A method of producing dextranase comprising culturing, in a fermentation medium, a dextranase-producing strain of the fungus Fusarium fusarioides, or a mutant or variant of said dextranase-producing strain, said strain being selected from the group consisting of Fusarium fusarioides I.M.I. No. 158,405, Dactylium fusarioides A.T.C.C. No. 14167, Fusarium chlamydosporum C.B.S. No. 145.25, Fusarium roseum A.T.C.C. No. 13379, and Fusarium tricinctum C.B.C. No. 261.51, and recovering the dextranase which accumulates therein.
Claim 4
Independent Claim
70 words
A method of producing dextranase comprising culturing, in a fermentation medium, a dextranase-producing strain of the fungus Fusarium fusarioides, or a mutant or variant of said dextranase-producing strain, said strain being selected from the group consisting of Fusarium fusarioides I.M.I. No. 158,405, Dactylium fusarioides A.T.C.C. No. 14167, Fusarium chlamydosporum C.B.S. No. 145.25, Fusarium roseum A.T.C.C. No. 13379, and Fusarium tricinctum C.B.C. No. 261.51, and recovering the dextranase which accumulates therein.
Claim 5
Dependent Claim
37 words
The method of claim 1 wherein culturing is effected by inoculating an aqueous nutrient fermentation medium with germinating spores of the fungus, the culture being incubated under aerated conditions at a predetermined temperature and level of pH.
Claim 6
Dependent Claim
47 words
The method of claim 3 wherein the predetermined temperature is in the range of 25*C. to 30*C., the pH is maintained below 7.8, and the aeration rate is such as to maintain in the culture medium a concentration of the dissolved oxygen between 20% and 30% saturation.
Claim 7
Dependent Claim
23 words
The method of claim 3 wherein the aqueous nutrient medium is an aerated sterile medium containing sources of assimilable carbon, nitrogen, and minerals.
Claim 8
Dependent Claim
17 words
The method of claim 5 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
Claim 9
Dependent Claim
37 words
The method of claim 2 wherein culturing is effected by inoculating an aqueous nutrient fermentation medium with germinating spores of the fungus, the culture being incubated under aerated conditions at a predetermined temperature and level of pH.
Claim 10
Dependent Claim
47 words
The method of claim 7 wherein the predetermined temperature is in the range of 25*C. to 30*C., the pH is maintained below 7.8, and the aeration rate is such as to maintain in the culture medium a concentration of the dissolved oxygen between 20% and 30% saturation.
Claim 11
Dependent Claim
23 words
The method of claim 7 wherein the aqueous nutrient medium is an aerated sterile medium containing sources of assimilable carbon, nitrogen, and minerals.
Claim 12
Dependent Claim
23 words
The method of claim 4 wherein the aqueous nutrient medium is an aerated sterile medium containing sources of assimilable carbon, nitrogen, and minerals.
Claim 13
Dependent Claim
23 words
The method of claim 8 wherein the aqueous nutrient medium is an aerated sterile medium containing sources of assimilable carbon, nitrogen, and minerals.
Claim 14
Dependent Claim
17 words
The method of claim 11 wherein the source of assimilable carbon include, as a dextranase inducer, dextran.
Claim 15
Dependent Claim
17 words
The method of claim 10 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
Claim 16
Dependent Claim
17 words
The method of claim 11 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
Claim 17
Dependent Claim
14 words
The method of claim 2 wherein the strain is Fusarium fusarioides I.M.I. No. 158,405.
Claim 18
Dependent Claim
37 words
The method of claim 15 wherein culturing is effected by inoculating an aqueous nutrient fermentation medium with germinating spores of the strain, the culture being incubated under aerated conditions at a predetermined temperature and level of pH.
Claim 19
Dependent Claim
23 words
The method of claim 16 wherein the aqueous nutrient medium is an aerated sterile medium containing sources of assimilable carbon, nitrogen and minerals.
Claim 20
Dependent Claim
17 words
The method of claim 17 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
Claim 21
Dependent Claim
14 words
The method of claim 2 wherein the strain is Dactylium fusarioides A.T.C.C. No. 14167.
Claim 22
Dependent Claim
37 words
The method of claim 19 wherein culturing is effected by inoculating an aqueous nutrient fermentation medium with germinating spores of the strain, the culture being incubated under aerated conditions at a predetermined temperature and level of pH.
Claim 23
Dependent Claim
22 words
The method of claim 20 wherein the aqueous nutrient medium is an aerated medium containing sources of assimilable carbon, nitrogen and minerals.
Claim 24
Dependent Claim
17 words
The method of claim 21 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
Claim 25
Dependent Claim
14 words
The method of claim 2 wherein the strain is Fusarium chlamydosporum C.B.S. No. 145.25.
Claim 26
Dependent Claim
37 words
The method of claim 23 wherein culturing is effected by inoculating an aqueous nutrient fermentation medium with germinating spores of the strain, the culture being incubated under aerated conditions at a predetermined temperature and level of pH.
Claim 27
Dependent Claim
22 words
The method of claim 24 wherein the aqueous nutrient medium is an aerated medium containing sources of assimilable carbon, nitrogen and minerals.
Claim 28
Dependent Claim
17 words
The method of claim 25 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
Claim 29
Dependent Claim
14 words
The method of claim 2 wherein the strain is Fusarium roseum A.T.C.C. NO. 13379.
Claim 30
Dependent Claim
37 words
The method of claim 27 wherein culturing is effected by inoculating an aqueous nutrient fermentation medium with germinating spores of the strain, the culture being incubated under aerated conditions at a predetermined temperature and level of pH.
Claim 31
Dependent Claim
22 words
The method of claim 28 wherein the aqueous nutrient medium is an aerated medium containing sources of assimilable carbon, nitrogen and minerals.
Claim 32
Dependent Claim
17 words
The method of claim 29 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
Claim 33
Dependent Claim
14 words
The method of claim 2 wherein the strain is Fusarium tricinctum C.B.C. No. 261.51.
Claim 34
Dependent Claim
36 words
The method of claim 31 wherein culturing is effected by inoculating an aqueous fermentation medium with germinating spores of the strain, the culture being incubated under aerated conditions at a predetermined temperature and level of pH.
Claim 35
Dependent Claim
22 words
The method of claim 32 wherein the aqueous nutrient medium is an aerated medium containing spores of assimilable carbon, nitrogen and minerals.
Claim 36
Dependent Claim
17 words
The method of claim 33 wherein the sources of assimilable carbon include, as a dextranase inducer, dextran.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-31 | 1978-09-30 | 1979-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-31 | 1982-09-30 | 1983-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-31 | 1986-09-30 | 1987-04-01 | $7700.00 | Window Closed / Lapsed |