Method of producing dextranase

Abstract

Abstract The instant disclosure relates to a method of obtaining an extracellular endodextranase enzyme. The method involves inoculating into an aqueous nutrient medium containing dextran and an assimilable nitrogen source, thereby forming a culture, an aerobic bacterial strain which has the following characteristics: GRAM REACTION - POSITIVE SHAPE - RODS MOTILITY - POSITIVE SPORES - SPORULATION DIFFICULT TO DEMONSTRATE IN NUTRIENT AGAR BUT EASIER IN GROWTH LIMITING MEDIUM SUCH AS CITRATE, SPORANGIA DEFINITELY SWOLLEN AGAR COLONIES - ROUND, OPAQUE GLUCOSE AGAR - POSITIVE FOR GROWTH, ACID FROM GLUCOSE USING PEPTONE AS A SOURCE OF NITROGEN GROWTH FACTORS - SLIGHT IF ANY GROWTH IN MINIMAL MEDIA CATALASE - POSITIVE LECITHIN - NO HYDROLYTIC ZONE FORMED MILK AGAR - POSITIVE ZONE OF HYDROLYSIS STARCH - POSITIVE ZONE OF HYDROLYSIS GELATINE AGAR - POSITIVE ZONE OF HYDROLYSIS GLUCOSE ASPARAGINE AGAR - SCANT GROWTH SOYBEAN AGAR - POSITIVE FOR GROWTH NUTRIENT BROTH - UNIFORM TURBIDITY FOLLOWED BY CLEARING AND FORMATION OF SEDIMENT SODIUM CHLORIDE (NaCl) broth - after 2 days incubation, growth in 5% NaCl broth, no growth in 7% NaCl broth NITRITES - NOT PRODUCED FROM NITRATES CITRATE - NOT UTILIZED TYROSINE AGAR - RED-BROWN PIGMENT AFTER 2 DAYS. The culture is then incubated until extracellular endodextranase is elaborated therein. The bacteria are thereafter separated, thereby leaving a solution containing the extracellular endodextranase enzyme. The aerobic bacterial strains used in this method are also disclosed.

Inventors

• 👤 R Davis
• 👤 D Isenberg

Bibliographic Details