US4497900A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassay for Neisseria gonorrhoeae antigens
Assignee: Abbott Laboratories
•
Filing: 1983-03-28
•
Granted: 1985-02-05
•
Expires: 2003-03-28
Abstract
Abstract The present disclosure relates to a solid phase immunoassay for the detection of Neisseria gonorrhoeae antigens in a clinical specimen, wherein the Neisseria gonorrhoeae antigens to be determined are coated or adsorbed on the solid phase.
Inventors
- 👤 Dinah Abram
- 👤 Alan S. Armstrong
- 👤 John E. Herrmann
- 👤 Chung-Mei Ling
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 21:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871; Y10S435/962; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/815; Y10S436/823; Y10S436/825
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
129 words
A method for determining Neisseria gonorrhoeae antigen in a clinical specimen comprising: (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen; (b) coating a bare solid support with the Neisseria gonorrhoeae antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with gonococcal antibody to form antigen-gonococcal antibody complex on the solid support; (e) separating the antigen-gonococcal antibody complex from unbound gonococcal antibody; (f) treating the antigen-gonococcal antibody complex with labeled antiglobulin, to form an antigen-gonococcal antibody-labeled antiglobulin complex on the solid support; (g) separating the antigen-gonococcal antibody-labeled antiglobulin complex from unbound labeled antiglobulin; and (h) determining the labeled antiglobulin bound to the antigen-gonococcal antibody complex as a measure of the Neisseria gonorrhoeae antigen in the specimen.
Claim 2
Independent Claim
129 words
A method for determining Neisseria gonorrhoeae antigen in a clinical specimen comprising: (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen; (b) coating a bare solid support with the Neisseria gonorrhoeae antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with gonococcal antibody to form antigen-gonococcal antibody complex on the solid support; (e) separating the antigen-gonococcal antibody complex from unbound gonococcal antibody; (f) treating the antigen-gonococcal antibody complex with labeled antiglobulin, to form an antigen-gonococcal antibody-labeled antiglobulin complex on the solid support; (g) separating the antigen-gonococcal antibody-labeled antiglobulin complex from unbound labeled antiglobulin; and (h) determining the labeled antiglobulin bound to the antigen-gonococcal antibody complex as a measure of the Neisseria gonorrhoeae antigen in the specimen.
Claim 3
Dependent Claim
21 words
A method according to claim 1 wherein the Neisseria gonorrhoeae cells are lysed using a buffered saline solution and a surfactant.
Claim 4
Dependent Claim
13 words
A method according to claim 2 wherein the surfactant is a deoxycholate salt.
Claim 5
Dependent Claim
13 words
A method according to claim 3 wherein the deoxycholate salt is sodium deoxycholate.
Claim 6
Dependent Claim
23 words
A method according to claim 1 wherein the gonococcal antibody is produced by immunizing an animal with a pool of Neisseria gonorrhoeae strains.
Claim 7
Dependent Claim
14 words
A method according to claim 1 wherein the antiglobulin is labeled with an enzyme.
Claim 8
Dependent Claim
18 words
A method according to claim 1 wherein the separation steps are performed by aspiration and washing with water.
Claim 9
Independent Claim
160 words
A method for determining Neisseria gonorrhoeae antigen in a clinical specimen comprising: (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen; (b) coating a bare solid support with the Neisseria gonorrhoeae antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with gonococcal antibody to form an antigen-gonococcal antibody complex on the solid support; (e) separating the antigen-gonococcal antibody complex from unbound gonococcal antibody; (f) treating the antigen-gonococcal antibody complex with antiglobulin, to form an antigen-gonococcal antibody-antiglobulin complex on the solid support; (g) separating the antigen-gonococcal antibody-antiglobulin complex from unbound antiglobulin; (h) treating the antigen-gonococcal antibody-antiglobulin complex with labeled antibody to the antiglobulin to form an antigen-gonococcal antibody-antiglobulin-labeled antibody complex on the solid support; (i) separating the antigen-gonococcal antibody-antigloblin-labeled antibody complex from unbound labeled antibody; and (j) determining the labeled antibody bound to the antigen-gonococcal antibody-antiglobulin complex as a measure of the Neisseria gonorrhoeae antigen in the specimen.
Claim 10
Independent Claim
160 words
A method for determining Neisseria gonorrhoeae antigen in a clinical specimen comprising: (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen; (b) coating a bare solid support with the Neisseria gonorrhoeae antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with gonococcal antibody to form an antigen-gonococcal antibody complex on the solid support; (e) separating the antigen-gonococcal antibody complex from unbound gonococcal antibody; (f) treating the antigen-gonococcal antibody complex with antiglobulin, to form an antigen-gonococcal antibody-antiglobulin complex on the solid support; (g) separating the antigen-gonococcal antibody-antiglobulin complex from unbound antiglobulin; (h) treating the antigen-gonococcal antibody-antiglobulin complex with labeled antibody to the antiglobulin to form an antigen-gonococcal antibody-antiglobulin-labeled antibody complex on the solid support; (i) separating the antigen-gonococcal antibody-antigloblin-labeled antibody complex from unbound labeled antibody; and (j) determining the labeled antibody bound to the antigen-gonococcal antibody-antiglobulin complex as a measure of the Neisseria gonorrhoeae antigen in the specimen.
Claim 11
Dependent Claim
21 words
A method according to claim 8 wherein the Neisseria gonorrhoeae cells are lysed using a buffered saline solution and a surfactant.
Claim 12
Dependent Claim
13 words
A method according to claim 9 wherein the surfactant is a deoxycholate salt.
Claim 13
Dependent Claim
13 words
A method according to claim 10 wherein the deoxycholate salt is sodium deoxycholate.
Claim 14
Dependent Claim
23 words
A method according to claim 8 wherein the gonococcal antibody is produced by immunizing an animal with a pool of Neisseria gonorrhoeae strains.
Claim 15
Dependent Claim
14 words
A method according to claim 8 wherein the antiglobulin is labeled with an enzyme.
Claim 16
Dependent Claim
18 words
A method according to claim 8 wherein the separation steps are performed by aspiration and washing with water.
Claim 17
Independent Claim
99 words
A method for determining Neisseria gonorrhoeae antigen in a clinical speciman comprising: (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen; (b) coating the bare solid support with the Neisseria gonorrhoeae antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with labeled gonococcal antibody; (e) separating the antigen coated solid support treated with labeled gonococcal antibody from unbound labeled gonococcal antibody; and (f) determining the labeled gonococcal antibody bound to the antigen coated solid support as a measure of the Neisseria gonorrhoeae antigen in the specimen.
Claim 18
Independent Claim
99 words
A method for determining Neisseria gonorrhoeae antigen in a clinical speciman comprising: (a) lysing Neisseria gonorrhoeae cells in the specimen to release Neisseria gonorrhoeae antigen; (b) coating the bare solid support with the Neisseria gonorrhoeae antigen; (c) separating the antigen coated solid support from the specimen; (d) treating the antigen coated solid support with labeled gonococcal antibody; (e) separating the antigen coated solid support treated with labeled gonococcal antibody from unbound labeled gonococcal antibody; and (f) determining the labeled gonococcal antibody bound to the antigen coated solid support as a measure of the Neisseria gonorrhoeae antigen in the specimen.
References Cited (Backward Citations)
US4066744A
Cited
US3974269A
Cited
US3951748A
Cited
US4186182A
Cited
US4029756A
Cited
US4115543A
Cited
US4166765A
Cited
US4150950A
Cited
US4140581A
Cited
US4111752A
Cited
US4188371A
Cited
US4241045A
Cited
US4332890A
Cited
US4351761A
Cited
US4273756A
Cited
US4292403A
Cited
US4443431A
Cited
US4390622A
Cited
US4446230A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-05 | 1988-08-06 | 1989-02-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-05 | 1992-08-06 | 1993-02-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-05 | 1996-08-06 | 1997-02-05 | $7700.00 | Window Closed / Lapsed |