US4241045A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purified antigen to test for Neisseria gonorrheae antibodies
Assignee: Research Corp
•
Filing: 1978-05-15
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Granted: 1980-12-23
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Expires: 1998-05-15
Abstract
Abstract Serological method for determining the presence of Neisseria gonorrhoeae antibodies in human sera and products utilized in such testing.
Inventors
- 👤 Hassan A. Gaafar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 16:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; A; A61; A61K; A61K39/00; A61K39/02; A61K39/095; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/12; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S530/00; Y10S530/806; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
181 words
A serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises preparing a composition containing undiluted serum or diluted in a physiologically acceptable liquid at a dilution of about 1:2 to 1:1000, incubating with a composition containing a species specific antigen preparation produced from a growth culture of Neisseria gonorrhoea to form an antigen-antibody conjugate when said antibodies are present; and detecting the presence of said conjugate; the antigen being protein in nature and characterized as follows: 1. stable in boiling water for a period of one hour, 2. stable in an aqueous medium at pH values of 3-11, 3. stable when incubated with the following enzymes; DNA--ase RNA--ase dextransase neuraminidase lysosyme, 4. isoelectric point of 4±0.2, 5. molecular weight of active subunits of 37,000-40,000 as determined by SDS-polyacrylamide gel, 6. contains 2-3% organic phosphorous, 7. soluble in water, and more soluble in aqueous media containing surface active agents; and insoluble in methanol, chloroform, and acetone, 8. reacts specifically with antibodies produced in rabbits against purified L-antigen, 9. partially soluble in 5% to 10% trichloroacetic acid.
Claim 2
Dependent Claim
181 words
A serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises preparing a composition containing undiluted serum or diluted in a physiologically acceptable liquid at a dilution of about 1:2 to 1:1000, incubating with a composition containing a species specific antigen preparation produced from a growth culture of Neisseria gonorrhoea to form an antigen-antibody conjugate when said antibodies are present; and detecting the presence of said conjugate; the antigen being protein in nature and characterized as follows: 1. stable in boiling water for a period of one hour, 2. stable in an aqueous medium at pH values of 3-11, 3. stable when incubated with the following enzymes; DNA--ase RNA--ase dextransase neuraminidase lysosyme, 4. isoelectric point of 4±0.2, 5. molecular weight of active subunits of 37,000-40,000 as determined by SDS-polyacrylamide gel, 6. contains 2-3% organic phosphorous, 7. soluble in water, and more soluble in aqueous media containing surface active agents; and insoluble in methanol, chloroform, and acetone, 8. reacts specifically with antibodies produced in rabbits against purified L-antigen, 9. partially soluble in 5% to 10% trichloroacetic acid.
Claim 3
Dependent Claim
32 words
A serological method as in claim 1 wherein the presence of said conjugate is detected by reaction with an anti-human IgG labelled with a chemical which fluoresces when exposed to ultraviolet light.
Claim 4
Dependent Claim
19 words
A method of claim 2 wherein the chemical is selected from the group consisting of fluorescein, rhodamine and auramine.
Claim 5
Dependent Claim
20 words
A method as in claim 2 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21823.
Claim 6
Dependent Claim
20 words
A method as in claim 2 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21824.
Claim 7
Dependent Claim
20 words
A method as in claim 2 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21825.
Claim 8
Dependent Claim
25 words
A Serological method as in claim 1 wherein the presence of said conjugate is detected by reaction with an anti-human IgG labelled with an enzyme.
Claim 9
Dependent Claim
32 words
A method as in claim 7 wherein the enzyme is selected from the group consisting of peroxidase, B-glucuronidase, B-D-glucosidase, B-D-galactosidase, urease, glucose oxidase plus peroxidase, galactose oxidase plus peroxidase, and acid phosphatase.
Claim 10
Dependent Claim
20 words
A method as in claim 7 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21823.
Claim 11
Dependent Claim
20 words
A method as in claim 7 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21824.
Claim 12
Dependent Claim
20 words
A method as in claim 7 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21825.
Claim 13
Dependent Claim
26 words
A serological method as in claim 1 wherein the presence of said conjugate is detected by reaction with an anti-human IgG labelled with a radioactive element.
Claim 14
Dependent Claim
30 words
A method as in claim 12 wherein the radioactive element is selected from the group consisting of 14 C , 125 I , 131 I , and 35 S .
Claim 15
Dependent Claim
20 words
A method as in claim 12 wherein the chemical is selected from the group consisting of fluorescein, rhodamine and auramine.
Claim 16
Dependent Claim
20 words
A method as in claim 14 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21823.
Claim 17
Dependent Claim
20 words
A method as in claim 14 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21824.
Claim 18
Dependent Claim
20 words
A method as in claim 14 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21825.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-23 | 1984-06-23 | 1984-12-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-23 | 1988-06-23 | 1988-12-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-23 | 1992-06-23 | 1992-12-23 | $7700.00 | Window Closed / Lapsed |