US4273756A
Expired / Lapsed
US
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Immunoassay for class specific antibodies
Assignee: Abbott Laboratories
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Filing: 1978-07-28
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Granted: 1981-06-16
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Expires: 1998-07-28
Abstract
Abstract The present invention encompasses a method for determining a class specific antibody to an antigen comprising: (a) binding an antibody for a specific immunoglobulin class to a solid support to form a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) removing unbound test sample to provide an antigen reagent, and (d) reacting the antigen reagent with a known antigen and measuring the amount of bound antigen thereby determining the amount of class specific antibody to that antigen. The present invention is useful for differential diagnosis, since the relative amount of class specific antibodies varies with the progress of infection.
Inventors
- 👤 Chung-Mei Ling
- 👤 Richard H. Decker
- 👤 Ruben Chairez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 20:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/543; G01N33/54306; G01N33/68; G01N33/6854; Y; Y02; Y02A; Y02A50/00; Y02A50/30; Y10; Y10S; Y10S436/00; Y10S436/82
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
88 words
A method for determining a class specific antibody to an antigen comprising: (a) binding an antibody for a specific immunoglobulin class to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) removing unbound test sample to provide an antigen reagent; (d) reacting the antigen reagent with a known labeled antigen, and (e) removing unbound labeled antigen and measuring the amount of bound labeled antigen, thereby determining the amount of class specific antibody to the known antigen.
Claim 2
Independent Claim
88 words
A method for determining a class specific antibody to an antigen comprising: (a) binding an antibody for a specific immunoglobulin class to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) removing unbound test sample to provide an antigen reagent; (d) reacting the antigen reagent with a known labeled antigen, and (e) removing unbound labeled antigen and measuring the amount of bound labeled antigen, thereby determining the amount of class specific antibody to the known antigen.
Claim 3
Dependent Claim
88 words
A method, according to claim 1, for determining IgM antibody to hepatitis A virus antigen comprising: (a) binding non-human species anti-μ chain of IgM antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample and washing to remove unbound test sample and provide an antigen reagent; (c) reacting the antigen reagent with labeled hepatitis A virus antigen and washing to remove unbound labeled hepatitis A virus antigen, and (d) measuring bound labeled hepatitis A virus antigen.
Claim 4
Dependent Claim
88 words
A method according to claim 1, for determining IgG antibody to hepatitis A virus antigen comprising: (a) binding non-human species anti-γ chain of IgG antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample and washing to remove unbound test sample to provide an antigen reagent; (c) reacting the antigen reagent with labeled hepatitis A virus antigen and washing to remove unbound labeled hepatitis A virus antigen, and (d) measuring bound labeled hepatitis A virus antigen.
Claim 5
Dependent Claim
85 words
A method, according to claim 1, for determining IgA antibody to labeled hepatitis A antigen comprising: (a) binding non-human species anti-α chain of IgA antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample and washing to remove unbound test sample and provide an antigen reagent; (c) reacting the antigen reagent with labeled hepatitis A antigen and washing to remove unbound labeled hepatitis A antigen, and (d) measuring bound labeled hepatitis A antigen.
Claim 6
Dependent Claim
84 words
A method according to claim 1, for determining IgG antibody to hepatitis B antigen comprising: (a) binding non-human species anti-γ chain of IgG antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample and washing to remove unbound test sample and provide an antigen reagent; (c) reacting the antigen reagent with labeled hepatitis B antigen and washing to remove unbound labeled hepatitis B antigen, and (d) measuring bound labeled hepatitis B antigen.
Claim 7
Dependent Claim
84 words
A method, according to claim 1, for determining IgM antibody to hepatitis B antigen comprising: (a) binding non-human species anti-μ chain of IgM antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample and washing to remove unbound test sample to provide an antigen reagent; (c) reacting the antigen reagent with labeled hepatitis B antigen and washing to remove unbound labeled hepatitis B antigen, and (d) measuring bound labeled hepatitis B antigen.
Claim 8
Dependent Claim
84 words
A method according to claim 1, for determining IgA antibody to hepatitis B antigen comprising: (a) binding non-human species anti-α chain of IgA antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample and washing to remove unbound test sample to provide an antigen reagent; (c) reacting the antigen reagent with labeled hepatitis B antigen and washing to remove unbound labeled hepatitis B antigen, and (d) measuring bound labeled hepatitis B antigen.
Claim 9
Independent Claim
102 words
A method for determining a class specific antibody to an antigen comprising: (a) binding an antibody for a specific immunoglobulin class to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) removing unbound test sample to provide an antigen reagent; (d) reacting the antigen reagent with a known antigen; (e) removing unbound antigen; and (f) reacting the bound antigen with a labeled antibody to the known antigen, removing the unbound labeled antibody and determining the bound labeled antibody, thereby determining the amount of class specific antibody to the known antigen.
Claim 10
Independent Claim
102 words
A method for determining a class specific antibody to an antigen comprising: (a) binding an antibody for a specific immunoglobulin class to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) removing unbound test sample to provide an antigen reagent; (d) reacting the antigen reagent with a known antigen; (e) removing unbound antigen; and (f) reacting the bound antigen with a labeled antibody to the known antigen, removing the unbound labeled antibody and determining the bound labeled antibody, thereby determining the amount of class specific antibody to the known antigen.
Claim 11
Dependent Claim
112 words
A method according to claim 8 for determining IgM antibody to hepatitis A virus antigen comprising: (a) binding non-human species anti-μ chain of IgM antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) washing class specific antibody reagent reacted with test sample to provide an antigen reagent; (d) reacting the antigen reagent with hepatitis A virus antigen and washing with water to remove unbound hepatitis A virus antigen; (e) reacting the product of (d) with labeled antibody to hepatitis A virus antigen and washing to remove unbound labeled antibody, and (f) measuring the amount of bound labeled antibody.
Claim 12
Dependent Claim
112 words
A method according to claim 8 for determining IgA antibody to hepatitis A virus antigen comprising: (a) binding non-human species anti-α chain of IgA antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) washing class specific antibody reagent reacted with test sample to provide an antigen reagent; (d) reacting the antigen reagent with hepatitis A virus antigen and washing with water to remove unbound hepatitis A virus angigen; (e) reacting the product of (d) with labeled antibody to hepatitis A virus antigen and washing to remove unbound labeled antibody, and (f) measuring the amount of bound labeled antibody.
Claim 13
Dependent Claim
111 words
A method according to claim 8 for determining IgG antibody to hepatitis A virus antigen comprising: (a) binding non-human species anti-γ chain of IgG, antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) washing class specific antibody reagent reacted with test sample to provide an antigen reagent; (d) reacting the antigen reagent with hepatitis A virus antigen and washing with water to remove unbound hepatitis A virus antigen; (e) reacting the product of (d) with labeled antibody to hepatitis A virus and washing to remove unbound labeled antibody, and (f) measuring the amount of bound labeled antibody.
Claim 14
Dependent Claim
112 words
A method according to claim 8 for determining IgM antibody to hepatitis B virus antigen comprising: (a) binding non-human species anti-μ chain of IgM antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) washing class specific antibody reagent reacted with test sample to provide an antigen reagent; (d) reacting the antigen reagent with hepatitis B virus antigen and washing with water to remove unbound hepatitis B virus antigen; (e) reacting the product of (d) with labeled antibody to hepatitis B virus antigen and washing to remove unbound labeled antibody, and (f) measuring the amount of bound labeled antibody.
Claim 15
Dependent Claim
112 words
A method according to claim 8 for determining IgA antibody to hepatitis B virus antigen comprising: (a) binding non-human species anti-α chain of IgA antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) washing class specific antibody reagent reacted with test sample to provide an antigen reagent; (d) reacting the antigen reagent with hepatitis B virus antigen and washing with water to remove unbound hepatitis B virus antigen; (e) reacting the product of (d) with labeled anti-body to hepatitis B virus antigen and washing to remove unbound labeled antibody, and (f) measuring the amount of bound labeled antibody.
Claim 16
Dependent Claim
112 words
A method according to claim 8 for determining IgG antibody to hepatitis B virus antigen comprising: (a) binding non-human species anti-γ chain of IgG antibody to a solid support to provide a class specific antibody reagent; (b) reacting the class specific antibody reagent with test sample; (c) washing class specific antibody reagent reacted with test sample to provide an antigen reagent; (d) reacting the antigen reagent with hepatitis B virus antigen and washing with water to remove unbound hepatitis B virus antigen; (e) reacting the product of (d) with labeled antibody to hepatitis B virus antigen and washing to remove unbound labeled antibody, and (f) measuring the amount of bound labeled antibody.
Claim 17
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for μ-chain of human IgM.
Claim 18
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for μ-chain of human IgM.
Claim 19
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for α-chain of human IgA.
Claim 20
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for α-chain of human IgA.
Claim 21
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for δ-chain of human IgD.
Claim 22
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for δ-chain of human IgD.
Claim 23
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for ε-chain of human IgE.
Claim 24
Dependent Claim
16 words
An immunoassay reagent comprising a solid support coated with antibody specific for ε-chain of human IgE.
References Cited (Backward Citations)
US3720760A
Cited
US3720760B1
Cited
US3941876A
Cited
US4031197A
Cited
US3904367A
Cited
US3959650A
Cited
US4048298A
Cited
US4020151A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |