Patent EP0888750A1 successfully refreshed from Google Patents.
US3951748A
Expired / Lapsed
US
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Sensitized matrix for detection of disease
Assignee: Medical Products Inc
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Filing: 1974-11-11
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Granted: 1976-04-20
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Expires: 1994-11-11
Abstract
Abstract A specific sensitized matrix for diagnosing both infectious and non-infectious disease is disclosed comprised of an insoluble, inert, pliable and wettable matrix having a network of pores, and a protein polymer network immobilized in the network of pores formed of protein carrier molecules, specific antigens or antibodies, and a coupling agent coupling the protein carrier molecules together and the antigen or antibody to the protein carrier molecules. A variety of matrix materials, protein carrier molecules, specific antigens or antibodies and coupling agents can be used. Methods of preparing the sensitized matrix and diagnostic test procedures utilizing the sensitized matrix for detecting disease are also disclosed.
Inventors
- 👤 Robert Francis Devlin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:35
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; B; B01; B01D; B01D69/00; B01D69/14; B01D69/141; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/811
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
83 words
A specific sensitized matrix for diagnosing disease comprising, an insoluble, inert matrix having a network of pores, carrier protein molecules, a specific immunological reactant selected from the group consisting of antigens and antibodies, and a coupling agent coupling the carrier protein molecules together, and coupling the specific immunological reactant to the carrier protein molecules and forming a network of the carrier protein molecules, the immunological reactant and the coupling agent, the last-mentioned network being immobilized within the network of pores of the matrix.
Claim 2
Dependent Claim
83 words
A specific sensitized matrix for diagnosing disease comprising, an insoluble, inert matrix having a network of pores, carrier protein molecules, a specific immunological reactant selected from the group consisting of antigens and antibodies, and a coupling agent coupling the carrier protein molecules together, and coupling the specific immunological reactant to the carrier protein molecules and forming a network of the carrier protein molecules, the immunological reactant and the coupling agent, the last-mentioned network being immobilized within the network of pores of the matrix.
Claim 3
Dependent Claim
21 words
The specific sensitized matrix of claim 1 where the matrix is polyurethane foam having at least 50% by volume open pores.
Claim 4
Dependent Claim
27 words
The specific sensitized matrix of claim 1 in which the coupling agent is glutaraldehyde in which residual reactive aldehyde groups are blocked by combination with amino groups.
Claim 5
Dependent Claim
14 words
The specific sensitized matrix of claim 3 where the amino groups are from glycine.
Claim 6
Dependent Claim
18 words
The specific sensitized matrix of claim 1 where the specific immunological reactant is derived from an infectious disease.
Claim 7
Dependent Claim
15 words
The specific sensitized matrix of claim 1 where the specific immunological reactant is gonococcus pili.
Claim 8
Dependent Claim
16 words
The specific sensitized matrix of claim 1 where the specific immunological reactant is an allergen extract.
Claim 9
Dependent Claim
18 words
The specific sensitized matrix of claim 1 where the specific immunological reactant is derived from a disease state.
Claim 10
Independent Claim
124 words
A method of preparing a specific sensitized matrix for diagnosing disease comprising, saturating at least a portion of an insoluble matrix having a network of pores with a buffered mixture having a pH of from about 4 to about 7, the buffered mixture comprising, protein carrier molecules, a specific immunological reactant selected from the group consisting of antigens and antibodies, and a coupling agent capable of bonding the protein carrier molecules together and of bonding the specific immunological reactant to the protein carrier molecules, incubating the saturated matrix so as to couple the protein carrier molecules and the specific immunological reactant together in a network immobilized in the network of pores of the matrix, and washing the matrix of unreacted components of the mixture.
Claim 11
Independent Claim
124 words
A method of preparing a specific sensitized matrix for diagnosing disease comprising, saturating at least a portion of an insoluble matrix having a network of pores with a buffered mixture having a pH of from about 4 to about 7, the buffered mixture comprising, protein carrier molecules, a specific immunological reactant selected from the group consisting of antigens and antibodies, and a coupling agent capable of bonding the protein carrier molecules together and of bonding the specific immunological reactant to the protein carrier molecules, incubating the saturated matrix so as to couple the protein carrier molecules and the specific immunological reactant together in a network immobilized in the network of pores of the matrix, and washing the matrix of unreacted components of the mixture.
Claim 12
Dependent Claim
41 words
The method of claim 9 where, the coupling agent is glutaraldehyde, and after washing, residual reactive aldehyde groups are blocked by combining them with amino groups from an amino acid, then washing the matrix of uncombined components of the amino acid.
Claim 13
Dependent Claim
11 words
The method of claim 10 where the amino acid is glycine.
Claim 14
Dependent Claim
43 words
The method of claim 9 where the matrix is polyurethane foam, the coupling agent is glutaraldehyde, and after washing, residual reactive aldehyde groups are blocked by combining them with amino groups from glycine, then washing the matrix of uncombined components of the glycine.
Claim 15
Dependent Claim
45 words
The method of claim 9 where the mixture is buffered with an acetate buffer solution having a pH of about 4.9. the coupling agent is a solution of glutaraldehyde and incubation is at about 4° C. for a period of about 2 to 24 hours.
Claim 16
Dependent Claim
16 words
The method of claim 9 where the specific immunological reactant is derived from an infectious disease.
Claim 17
Dependent Claim
13 words
The method of claim 9 where the specific immunological reactant is gonococcus pili.
Claim 18
Dependent Claim
14 words
The method of claim 13 where the specific immunological reactant is an allergen extract.
Claim 19
Dependent Claim
16 words
The method of claim 13 where the specific immunological reactant is derived from a disease state.
Claim 20
Independent Claim
153 words
A method of diagnosis of a specific disease comprising, incubating blood serum of a patient being tested for the specific disease with a sensitized matrix comprised of an insoluble, inert matrix having a network of pores and a network of protein carrier molecules and immunological reactant selected from the group consisting of antigens and antibodies and known to be specific to the disease coupled together by a coupling agent and immobilized within the network of pores, so as to bind the patient's blood serum to the sensitized matrix, washing the matrix of any unbound serum, then contacting the sensitized matrix with a reagent capable of catalyzing a measurable reaction, whereby said reagent is bound to any corresponding opposite reactant of the immunological reactant selected, washing any unbound reagent from the sensitized matrix, and then, subjecting the sensitized matrix to a suitable substrate thereby producing a measurable catalytic reaction for measurement of the reagent.
Claim 21
Independent Claim
153 words
A method of diagnosis of a specific disease comprising, incubating blood serum of a patient being tested for the specific disease with a sensitized matrix comprised of an insoluble, inert matrix having a network of pores and a network of protein carrier molecules and immunological reactant selected from the group consisting of antigens and antibodies and known to be specific to the disease coupled together by a coupling agent and immobilized within the network of pores, so as to bind the patient's blood serum to the sensitized matrix, washing the matrix of any unbound serum, then contacting the sensitized matrix with a reagent capable of catalyzing a measurable reaction, whereby said reagent is bound to any corresponding opposite reactant of the immunological reactant selected, washing any unbound reagent from the sensitized matrix, and then, subjecting the sensitized matrix to a suitable substrate thereby producing a measurable catalytic reaction for measurement of the reagent.
Claim 22
Independent Claim
146 words
A method of preparing a specific sensitized matrix for diagnosing disease comprising, saturating at least a portion of an insoluble matrix having a network of pores with a buffered mixture having a pH of from about 4 to 7 of protein carrier molecules and a coupling agent capable of bonding the protein carrier molecules together, incubating the saturated matrix so as to polymerize and couple together the protein carrier molecules, washing the matrix of unreacted components of the mixture, then contacting the matrix with a buffered mixture having a pH of from about 4 to 7 with a specific immunological reactant and additional coupling agent capable of bonding the specific immunological reactant to the protein carrier molecules polymerized in the matrix, then incubating the matrix so as to couple the specific immunological reactant to the protein carrier molecules, and then washing the matrix of unreacted components.
Claim 23
Independent Claim
146 words
A method of preparing a specific sensitized matrix for diagnosing disease comprising, saturating at least a portion of an insoluble matrix having a network of pores with a buffered mixture having a pH of from about 4 to 7 of protein carrier molecules and a coupling agent capable of bonding the protein carrier molecules together, incubating the saturated matrix so as to polymerize and couple together the protein carrier molecules, washing the matrix of unreacted components of the mixture, then contacting the matrix with a buffered mixture having a pH of from about 4 to 7 with a specific immunological reactant and additional coupling agent capable of bonding the specific immunological reactant to the protein carrier molecules polymerized in the matrix, then incubating the matrix so as to couple the specific immunological reactant to the protein carrier molecules, and then washing the matrix of unreacted components.
Claim 24
Dependent Claim
15 words
The method of claim 19 where the reactant is an enzyme labeled anti-human IgG serum.
Claim 25
Dependent Claim
27 words
The method of claim 19 where the reactant capable of catalyzing a measurable reaction is selected from the group consisting of anti-human IgG and IgG/IgM-peroxidase labeled serum.
Claim 26
Dependent Claim
26 words
The method of claim 21 where the measurable reaction is achieved by reacting the peroxidase, and the substrate is guaiacol in the presence of hydrogen peroxide.
Claim 27
Independent Claim
106 words
A method of diagnosis of a specific disease comprising, incubating blood serum of a patient being tested for the specific disease with a sensitized matrix comprised of an insoluble, inert matrix having a network of pores and a network of protein carrier molecules and immunological reactant selected from the group consisting of antigens and antibodies and known to be specific to the disease coupled together by a coupling agent and immobilized within the network of pores, so as to bind the patient's blood serum to the sensitized matrix, washing the matrix of any unbound serum, then incubating the sensitized matrix with fluoresceinisothiocyanate labeled anti-human IgG serum.
Claim 28
Independent Claim
106 words
A method of diagnosis of a specific disease comprising, incubating blood serum of a patient being tested for the specific disease with a sensitized matrix comprised of an insoluble, inert matrix having a network of pores and a network of protein carrier molecules and immunological reactant selected from the group consisting of antigens and antibodies and known to be specific to the disease coupled together by a coupling agent and immobilized within the network of pores, so as to bind the patient's blood serum to the sensitized matrix, washing the matrix of any unbound serum, then incubating the sensitized matrix with fluoresceinisothiocyanate labeled anti-human IgG serum.
Claim 29
Dependent Claim
16 words
The method of claim 23 including, subjecting the incubated sensitized matrix to a measurement of fluorescence.
Claim 30
Independent Claim
124 words
A method of diagnosis of a specific disease comprising, incubating blood serum of a patient being tested for the specific disease with a sensitized matrix comprised of an insoluble, inert matrix having a network of pores and a network of protein carrier molecules and immunological reactant selected from the group consisting of antigens and antibodies and known to be specific to the disease coupled together by a coupling agent and immobilized within the network of pores, so as to bind the patient's blood serum to the sensitized matrix, washing the matrix of any unbound serum, then incubating the sensitized matrix with an anti-human radioisotope labeled serum, and subjecting the incubated sensitized matrix to a measurement of radioactivity counter capable of measuring any reacted radioisotopes.
Claim 31
Independent Claim
124 words
A method of diagnosis of a specific disease comprising, incubating blood serum of a patient being tested for the specific disease with a sensitized matrix comprised of an insoluble, inert matrix having a network of pores and a network of protein carrier molecules and immunological reactant selected from the group consisting of antigens and antibodies and known to be specific to the disease coupled together by a coupling agent and immobilized within the network of pores, so as to bind the patient's blood serum to the sensitized matrix, washing the matrix of any unbound serum, then incubating the sensitized matrix with an anti-human radioisotope labeled serum, and subjecting the incubated sensitized matrix to a measurement of radioactivity counter capable of measuring any reacted radioisotopes.
References Cited (Backward Citations)
US3791932A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-20 | 1979-10-20 | 1980-04-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-20 | 1983-10-20 | 1984-04-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-20 | 1987-10-20 | 1988-04-20 | $7700.00 | Window Closed / Lapsed |