US4166765A
Expired / Lapsed
US
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Detecting Neisseria bacteria
Assignee: Corning Glass Works
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Filing: 1977-09-28
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Granted: 1979-09-04
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Expires: 1997-09-28
Abstract
Abstract Bacteria of the genus Neisseria can be detected in a sample by testing for the presence of an enzyme capable of oxidizing 1,2-propanediol and reducing NAD (nicotinamide-adenine-dinucleotide). The complete structure of the enzyme is not known but, because of those two characterizing properties, the nomenclature 1,2-propanediol dehydrogenase therefor is proposed herein.
Inventors
- 👤 Howard H. Weetall
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 10:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Q1/02; C12Q1/04; C12Q1/12; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
32 words
A method for detecting the presence of bacteria of the genus Neisseria in a fluid sample, the method comprising testing said sample for an enzyme capable of oxidizing 1,2-propanediol and reducing NAD.
Claim 2
Independent Claim
32 words
A method for detecting the presence of bacteria of the genus Neisseria in a fluid sample, the method comprising testing said sample for an enzyme capable of oxidizing 1,2-propanediol and reducing NAD.
Claim 3
Dependent Claim
52 words
A method according to claim 1 wherein the said fluid sample is a lysed human body fluid or exudate and the presence of said enzyme is determined by incubating the sample in a solution of 1,2-propanediol and NAD and determining whether a portion of the NAD is reduced by the lysed sample.
Claim 4
Dependent Claim
11 words
The method of claim 2 wherein the reduction is determined fluorometrically.
Claim 5
Dependent Claim
11 words
The method of claim 2 wherein the reduction is determined spectrophotometrically.
Claim 6
Independent Claim
67 words
A method of detecting the presence of Neisseria gonorrhoeae bacteria in a sample of human body fluid or exudate, the method comprising the steps of contacting the sample with a solution of lysing agent under conditions sufficient to cause the release of intracellular bacterial enzymes thereby forming a lysate and then testing the lysate for the presence of an enzyme capable of oxidizing 1,2-propanediol and reducing NAD.
Claim 7
Independent Claim
67 words
A method of detecting the presence of Neisseria gonorrhoeae bacteria in a sample of human body fluid or exudate, the method comprising the steps of contacting the sample with a solution of lysing agent under conditions sufficient to cause the release of intracellular bacterial enzymes thereby forming a lysate and then testing the lysate for the presence of an enzyme capable of oxidizing 1,2-propanediol and reducing NAD.
Claim 8
Dependent Claim
35 words
The method of claim 5 wherein the test for the presence of the said enzyme comprises incubating the lysate with a solution of the cofactor nicotinamide-adenine-dinucleotide and 1,2-propanediol and determining whether the cofactor is reduced.
Claim 9
Dependent Claim
13 words
The method of claim 6 wherein reduction of the cofactor is determined fluorometrically.
Claim 10
Dependent Claim
13 words
The method of claim 6 wherein reduction of the cofactor is determined spectrophotometrically.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-03 | 1983-03-05 | 1983-09-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-03 | 1987-03-05 | 1987-09-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-03 | 1991-03-05 | 1991-09-04 | $7700.00 | Window Closed / Lapsed |