US4039387A
Expired / Lapsed
US
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Medium and method for distinguishing Neisseria gonorrhoeae and Neisseria meningitidis
Assignee: Corning Glass Works
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Filing: 1976-07-01
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Granted: 1977-08-02
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Expires: 1996-07-01
Abstract
Abstract A microbial growth medium for distinguishing Neisseria gonorrhoeae from Neisseria meningitidis. The medium comprises a gonococci and meningococci specific medium having incorporated therein a quantity of 8-anilino-1-naphthalene-sulfonic acid (ANS) or a salt thereof, at a concentration sufficient to permit the growth of the meningococci and inhibit the growth of the gonococci.
Inventors
- 👤 Lynn B. Simpson
- 👤 Milton M. Takeguchi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 19:07
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/12; Y; Y10; Y10S; Y10S435/00; Y10S435/80; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849; Y10S435/852; Y10S435/871; Y10S435/873; Y10S435/911; Y10S435/921; Y10S435/922
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
56 words
A microbial growth medium specific to gonococci and meningococci bacteria, the medium having incorporated therein 8-anilino-1-naphthalene-sulfonic acid or a salt thereof in a quantity sufficient to permit the growth of meningococci bacteria and inhibit the growth of gonococci bacteria, the amount of 8-anilino-1-naphthalene-sulfonic acid or a salt thereof being at least about 0.08 mg./ml. of medium.
Claim 2
Dependent Claim
56 words
A microbial growth medium specific to gonococci and meningococci bacteria, the medium having incorporated therein 8-anilino-1-naphthalene-sulfonic acid or a salt thereof in a quantity sufficient to permit the growth of meningococci bacteria and inhibit the growth of gonococci bacteria, the amount of 8-anilino-1-naphthalene-sulfonic acid or a salt thereof being at least about 0.08 mg./ml. of medium.
Claim 3
Dependent Claim
29 words
The medium of claim 1 wherein the medium comprises a Thayer-Martin medium containing at least about 0.5 mg of 8-anilino-1-naphthalene-sulfonic acid, or a salt thereof, per ml of medium.
Claim 4
Dependent Claim
31 words
The medium of claim 1 wherein the medium comprises a New York City medium containing at least about 0.08 mg of 8-anilino-1-naphthalene-sulfonic acid or a salt thereof per ml of medium.
Claim 5
Dependent Claim
14 words
The medium of claim 1 wherein the 8-anilino-1-naphthalene-sulfonic acid is present as a salt.
Claim 6
Independent Claim
81 words
A method for the definitive identification of Neisseria gonorrhoeae in a specimen, the method comprising the steps of: A. incubating one portion of the specimen with a microbial growth medium specific to gonococci and meningococci bacteria; and B. incubating another portion of the specimen with a microbial growth medium specific to gonococci and meningococci bacteria and containing 8-anilino-1-naphthalene-sulfonic acid, or a salt thereof, in an amount sufficient to permit the growth of meningococci bacteria and inhibit the growth of gonococci bacteria.
Claim 7
Independent Claim
81 words
A method for the definitive identification of Neisseria gonorrhoeae in a specimen, the method comprising the steps of: A. incubating one portion of the specimen with a microbial growth medium specific to gonococci and meningococci bacteria; and B. incubating another portion of the specimen with a microbial growth medium specific to gonococci and meningococci bacteria and containing 8-anilino-1-naphthalene-sulfonic acid, or a salt thereof, in an amount sufficient to permit the growth of meningococci bacteria and inhibit the growth of gonococci bacteria.
Claim 8
Dependent Claim
26 words
The method of claim 5 wherein the medium of step (B) comprises a Thayer-Martin medium containing at least about 0.5% 8-anilino-1-naphthalene-sulfonic acid or a salt thereof.
Claim 9
Dependent Claim
34 words
The method of claim 5 wherein the medium of step (B) comprises a New York City medium containing at least about 0.08 mg of 8-anilino-1-naphthalene-sulfonic acid or a salt thereof per ml of medium.
Claim 10
Dependent Claim
19 words
The method of claim 5 wherein the incubation period of step (A) is for at least about 16-18 hours.
Claim 11
Dependent Claim
20 words
The method of claim 5 wherein the incubation period of step (B) is for at least 8 to 48 hours.
Claim 12
Dependent Claim
14 words
The method of claim 5 wherein the 8-anilino-1-naphthalene-sulfonic acid is present as a salt.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-01 | 1981-01-31 | 1981-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-01 | 1985-01-31 | 1985-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-01 | 1989-01-31 | 1989-08-02 | $7700.00 | Window Closed / Lapsed |