US4390622A
Expired / Lapsed
US
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Neisseria bacteria species identification and beta lactamase testing methods
Assignee: Individual
•
Filing: 1981-09-21
•
Granted: 1983-06-28
•
Expires: 2001-09-21
Abstract
Abstract Method for rapid, high intensity visual indication of the presence of Neisseria bacteria species utilizing a new and improved peptone buffer and pH indicator reagent system as the specimen carrier for oxidation series testing, which series test may also include beta lactamase enzyme detection utilizing the reaction of the same reagent system carrying the specimen with a penicillin substrate.
Inventors
- 👤 Garry W. Cartwright
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 02:37
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/12; C12Q1/34; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/22; G01N2333/90; G01N2333/914; G01N2333/978; G01N2333/986; G01N2415/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
89 words
A method for providing rapid, high intensity, visual indication of the presence of Neisseria bacteria species in a specimen comprising: the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, contacting the reagent system specimen suspension with at least one carbohydrate substrate taken from the class consisting of glucose, maltose, sucrose and lactose, permitting the reagent system specimen to react with the contacted carbohydrate substrate, and visually observing the reaction results for color change in a predetermined time period.
Claim 2
Independent Claim
89 words
A method for providing rapid, high intensity, visual indication of the presence of Neisseria bacteria species in a specimen comprising: the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, contacting the reagent system specimen suspension with at least one carbohydrate substrate taken from the class consisting of glucose, maltose, sucrose and lactose, permitting the reagent system specimen to react with the contacted carbohydrate substrate, and visually observing the reaction results for color change in a predetermined time period.
Claim 3
Dependent Claim
44 words
The method of claim 1 wherein a portion of the reagent system specimen suspension is contacted with each of said carbohydrate substrates for reaction of the reagent system specimen with the contacted carbohydrate substrate for visual observation of the reaction results for color change.
Claim 4
Dependent Claim
29 words
The method of claim 1 wherein the reaction results are visually observed within a time period of from between about two hours and about seven hours for color change.
Claim 5
Dependent Claim
23 words
The method of claim 3 wherein a color change to a yellow color indicates the presence of Neisseria bacteria species in the specimen.
Claim 6
Dependent Claim
30 words
The method of claim 4 wherein the Neisseria bacteria species undergoing testing is taken from the class consisting of N. gonorrhoeae, N. meningitidis, N. lactamica, N. sicca and N. catarrhalis.
Claim 7
Dependent Claim
26 words
The method of claim 1 wherein the peptone buffer and pH indicator reagent system has a pH within the range between about 7.1 and about 7.5.
Claim 8
Dependent Claim
14 words
The method of claim 6 wherein the peptone buffer is a proteose peptone material.
Claim 9
Dependent Claim
15 words
The method of claim 7 wherein the proteose peptone buffer is a beef peptone material.
Claim 10
Dependent Claim
15 words
The method of claim 8 wherein the proteose beef peptone buffer is peptone number 3.
Claim 11
Dependent Claim
28 words
The method of claim 7 wherein the proteose peptone is present in the reagent system in an amount of from between about 1.5% to about 2.5% by volume.
Claim 12
Dependent Claim
12 words
The method of claim 7 wherein the pH indicator is phenol red.
Claim 13
Dependent Claim
34 words
The method of claim 10 wherein the pH indicator is phenol red and is present in said aqueous reagent system in an amount of from between about 0.04 and about 0.085 grams per liter.
Claim 14
Independent Claim
75 words
The method of rapid, high intensity, visual indication of the presence of beta lactamase enzymes in a specimen comprising the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, contacting the reagent system specimen suspension with a penicillin substrate, permitting the reagent system specimen to react with the contact penicillin substrate, and visually observing the reaction results for color change in a predetermined time period.
Claim 15
Independent Claim
75 words
The method of rapid, high intensity, visual indication of the presence of beta lactamase enzymes in a specimen comprising the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, contacting the reagent system specimen suspension with a penicillin substrate, permitting the reagent system specimen to react with the contact penicillin substrate, and visually observing the reaction results for color change in a predetermined time period.
Claim 16
Dependent Claim
38 words
The method of claim 13 wherein the penicillin substrate is a salt of penicillin and the reaction results are visually observed within a time period of from between about five minutes and about seven hours for color change.
Claim 17
Dependent Claim
40 words
The method of claim 14 wherein the penicillin salt is taken from the class consisting of sodium and potassium penicillin G and wherein a color change to a yellow color indicates the presence of beta lactamase enzyme in the specimen.
Claim 18
Dependent Claim
30 words
The method of claim 15 wherein the Neisseria bacteria species undergoing testing is taken from the class consisting of N. gonorrhoeae, N. meningitidis, N. lactamica, N. sicca and N. catarrhalis.
Claim 19
Dependent Claim
26 words
The method of claim 13 wherein the peptone buffer and pH indicator reagent system has a pH within the range between about 7.1 and about 7.5.
Claim 20
Dependent Claim
14 words
The method of claim 17 wherein the peptone buffer is a proteose peptone material.
Claim 21
Dependent Claim
15 words
The method of claim 18 wherein the proteose peptone buffer is a beef peptone material.
Claim 22
Dependent Claim
15 words
The method of claim 19 wherein the proteose beef peptone buffer is peptone number 3.
Claim 23
Dependent Claim
28 words
The method of claim 18 wherein the proteose peptone is present in the reagent system in an amount of from between about 1.5% to about 2.5% by volume.
Claim 24
Dependent Claim
12 words
The method of claim 18 wherein the pH indicator is phenol red.
Claim 25
Dependent Claim
34 words
The method of claim 21 wherein the pH indicator is phenol red and is present in said aqueous reagent system in an amount of from between about 0.04 and about 0.085 grams per liter.
Claim 26
Independent Claim
110 words
A method of rapid, high intensity, visual indication of the presence of Neisseria bacteria species and of beta lactamase enzyme in a specimen comprising the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, which is contacted with a penicillin substrate, and with carbohydrate substrates separately, permitting the reagent system specimen to react with the contacted carbohydrate substrates and the penicillin substrate, and visually observing the reaction results for color change induced by the presence of one or more enzymes taken from the class consisting of N. gonorrhoeae, N. meningitidis, N. lactamica, and N. sicca, in a predetermined time period.
Claim 27
Independent Claim
110 words
A method of rapid, high intensity, visual indication of the presence of Neisseria bacteria species and of beta lactamase enzyme in a specimen comprising the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, which is contacted with a penicillin substrate, and with carbohydrate substrates separately, permitting the reagent system specimen to react with the contacted carbohydrate substrates and the penicillin substrate, and visually observing the reaction results for color change induced by the presence of one or more enzymes taken from the class consisting of N. gonorrhoeae, N. meningitidis, N. lactamica, and N. sicca, in a predetermined time period.
Claim 28
Dependent Claim
44 words
The method of claim 25 wherein a portion of the reagent system specimen suspension is contacted with each of said carbohydrate substrates for reaction of the reagent system specimen with the contacted carbohydrate substrate for visual observation of the reaction results for color change.
Claim 29
Dependent Claim
29 words
The method of claim 24 wherein the reaction results are visually observed within a time perod of from between about two hours and about seven hours for color change.
Claim 30
Dependent Claim
25 words
The method of claim 26 wherein color change to a yellow color indicates the presence of Neisseria bacteria species and beta lactamase in the specimen.
Claim 31
Dependent Claim
30 words
The method of claim 27 wherein the Neisseria bacteria species undergoing testing is taken from the class consisting of N. gonorrhoeae, N. meningitidis, N. lactamica, N. sicca and N. catarrhalis.
Claim 32
Dependent Claim
26 words
The method of claim 24 wherein the peptone buffer and pH indicator reagent system has a pH within the range between about 7.1 and about 7.5.
Claim 33
Dependent Claim
14 words
The method of claim 29 wherein the peptone buffer is a proteose peptone material.
Claim 34
Dependent Claim
15 words
The method of claim 30 wherein the proteose peptone buffer is a beef peptone material.
Claim 35
Dependent Claim
15 words
The method of claim 31 wherein the proteose beef peptone buffer is peptone number 3.
Claim 36
Dependent Claim
28 words
The method of claim 30 wherein the proteose peptone is present in the reagent system in an amount of from between about 1.5% to about 2.5% by volume.
Claim 37
Dependent Claim
12 words
The method of claim 30 wherein the pH indicator is phenol red.
Claim 38
Dependent Claim
34 words
The method of claim 33 wherein the pH indicator is phenol red and is present in said aqueous reagent system in an amount of from between about 0.04 and about 0.085 grams per liter.
References Cited (Backward Citations)
US4234683A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-27 | 1986-12-27 | 1987-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-27 | 1990-12-27 | 1991-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-27 | 1994-12-27 | 1995-06-28 | $7700.00 | Window Closed / Lapsed |