US4234683A
Expired / Lapsed
US
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Beta-lactamase diagnostic product and method
Assignee: Individual
•
Filing: 1978-11-24
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Granted: 1980-11-18
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Expires: 1998-11-24
Abstract
Abstract A diagnostic product and method are provided for rapid, sensitive and reproducible screening of penicillin resistance in bacteriological species such as Neisseria gonorrhoeae and Haemophilus influenzae. The diagnostic product and method of the present invention are utilized with aqueous solutions of iodine. The product includes four components; a porous support; a starch; a substrate such as penicillin and, a nucleophilic reagent. Preferred embodiments include a particular nucleophilic reagent which provides assay results in about 10 to about 30 seconds. The best mode contemplated for the diagnostic product is an embodiment in the form of a kit.
Inventors
- 👤 William A. McMillan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 14:08
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/978; G01N2333/986; G01N2415/00; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/141111
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
39 words
A diagnostic product useful for sensitive, reproducible assays of microorganisms which produce beta-lactamase comprising: a porous support on which is absorbed a substrate component, a starch component and a water-soluble thiosulfate component, the substrate component having a beta-lactamase ring.
Claim 2
Dependent Claim
39 words
A diagnostic product useful for sensitive, reproducible assays of microorganisms which produce beta-lactamase comprising: a porous support on which is absorbed a substrate component, a starch component and a water-soluble thiosulfate component, the substrate component having a beta-lactamase ring.
Claim 3
Dependent Claim
29 words
A diagnostic product as in claim 1 wherein the beta-lactam ring of the substrate component is condensed to a ring selected from the group consisting of thiazolidiene and dihydrothiazine.
Claim 4
Dependent Claim
31 words
A diagnostic product as in claim 2 wherein the substrate component has the structure ##STR8## and R 2 is selected from the group consisting of benzyl, phenoxylmethyl, α-aminobenzyl, α-carboxybenzyl and dimethyoxyphenyl.
Claim 5
Dependent Claim
12 words
The diagnostic product as in claim 3 wherein R 2 is benzyl.
Claim 6
Dependent Claim
26 words
A diagnostic product as in claim 1 wherein the substrate has the structure: ##STR9## wherein R 1 is a substituted amide of an aromatic carboxylic acid.
Claim 7
Dependent Claim
38 words
The diagnostic product as in claim 1 wherein said porous support has said substrate component, said starch component and said thiosulfate component upon the support in a weight ratio of substrate to starch to thiosulfate of about 4-20/0.2-1.0/0.5-2.2.
Claim 8
Dependent Claim
20 words
The diagnostic product of claim 1 wherein the support is selected from the group consisting of cellulose, agar, and agarose.
Claim 9
Dependent Claim
11 words
The diagnostic product of claim 1 wherein the support is frozen.
Claim 10
Dependent Claim
23 words
The diagnostic product as in claim 8 wherein the frozen support is at a temperature from about -20° C. to about -70° C.
Claim 11
Dependent Claim
119 words
A diagnostic product, useful for assaying for microorganisms which produce beta-lactamase, said product formed by the steps comprising: absorbing upon a porous support a substrate component, a starch component, and a thiosulfate component, the absorbing by exposing the support to an aqueous solution in which the components are dissolved, the aqueous solution including from about 4 to about 20 wt.% of the substrate component, from about 0.2 to about 1.0 wt.% of the starch component and from about 0.05 to about 0.20 M of the thiosulfate component, wherein the substrate has the structure ##STR10## and R 2 is selected from the group consisting of benzyl, phenoxylmethyl, α-aminobenzyl, α-carboxybenzyl, and dimethyloxyphenyl; and; retrieving the porous support from the aqueous solution.
Claim 12
Dependent Claim
119 words
A diagnostic product, useful for assaying for microorganisms which produce beta-lactamase, said product formed by the steps comprising: absorbing upon a porous support a substrate component, a starch component, and a thiosulfate component, the absorbing by exposing the support to an aqueous solution in which the components are dissolved, the aqueous solution including from about 4 to about 20 wt.% of the substrate component, from about 0.2 to about 1.0 wt.% of the starch component and from about 0.05 to about 0.20 M of the thiosulfate component, wherein the substrate has the structure ##STR10## and R 2 is selected from the group consisting of benzyl, phenoxylmethyl, α-aminobenzyl, α-carboxybenzyl, and dimethyloxyphenyl; and; retrieving the porous support from the aqueous solution.
Claim 13
Dependent Claim
17 words
The diagnostic product as in claim 10 further comprising freezing the porous support after the retrieving step.
Claim 14
Dependent Claim
22 words
The diagnostic product as in claim 11 wherein the freezing is at a temperature from about -20° C. from about -70° C.
Claim 15
Dependent Claim
76 words
In an assaying method for distinguishing microorganisms with resistance to an antiobiotic, the antibiotic including a beta-lactam ring, wherein a starch and the antibiotic are absorbed upon a porous support, the porous support is flooded with an aqueous solution of iodine, and a microorganism culture is applied to the porous support, the improvement comprising: impregnating the support with a water soluble thiosulfate compound, the impregnating prior to the application of the microorganism culture to the support.
Claim 16
Dependent Claim
76 words
In an assaying method for distinguishing microorganisms with resistance to an antiobiotic, the antibiotic including a beta-lactam ring, wherein a starch and the antibiotic are absorbed upon a porous support, the porous support is flooded with an aqueous solution of iodine, and a microorganism culture is applied to the porous support, the improvement comprising: impregnating the support with a water soluble thiosulfate compound, the impregnating prior to the application of the microorganism culture to the support.
Claim 17
Dependent Claim
28 words
The method as in claim 13 wherein the impregnating comprises: dissolving the thiosulfate compound in an aqueous solution, exposing the support thereto, and retrieving and draining the support.
Claim 18
Dependent Claim
30 words
The method as in claim 14 wherein the aqueous solution to which the support is exposed further includes said starch and said substrate dissolved therein for absorption upon said support.
Claim 19
Dependent Claim
27 words
The method as in claim 13 further comprising: freezing the support prior to flooding, the freezing at a temperature from about -20° C. to about -70° C.
Claim 20
Dependent Claim
46 words
A diagnostic kit useful for assaying penicillin resistant bacteriological species, comprising: a moisture tight ampoule, the ampoule enclosing two components, the first component comprising a freeze-dried penicillin and the second component comprising an anhydrous water soluble thiosulfate compound; and, a porous support having starch absorbed thereon.
Claim 21
Dependent Claim
46 words
A diagnostic kit useful for assaying penicillin resistant bacteriological species, comprising: a moisture tight ampoule, the ampoule enclosing two components, the first component comprising a freeze-dried penicillin and the second component comprising an anhydrous water soluble thiosulfate compound; and, a porous support having starch absorbed thereon.
Claim 22
Dependent Claim
15 words
The diagnostic kit as in claim 17 wherein the second component comprises anhydrous sodium thiosulfate.
Claim 23
Dependent Claim
27 words
The diagnostic kit as in claim 17 including a means for holding the supports to which water and the contents of the ampoule can be added thereto.
Claim 24
Dependent Claim
18 words
The diagnostic kit as in claim 19 wherein said means is a tray and further includes a lid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-18 | 1984-05-19 | 1984-11-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-18 | 1988-05-19 | 1988-11-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-18 | 1992-05-19 | 1992-11-18 | $7700.00 | Window Closed / Lapsed |