US4446230A
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Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae
Assignee: Temple University
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Filing: 1981-10-30
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Granted: 1984-05-01
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Expires: 2001-10-30
Abstract
Abstract A strain of Neisseria gonorrhoeae ATCC 31953 is described which is abnormal in that it has characteristically poor growth on chocolate agar at a temperature range of about 30 DEG C. to about 37 DEG C. in a CO2 atmosphere suitable for growth of N. gonorrhoeae. This strain is resistant to nalidixic acid at the 5-10 mcg/ml level and resistant to streptomycin at the 1000 mcg/ml level or greater. N. gonorrhoeae ATCC 31953 is a test strain suitable for use in the method described for the laboratory diagnosis of gonorrhea. The method comprises the steps of (1) applying a non-toxic preparation of a patient's specimen material, directly to a culture of Neisseria gonorrhoeae ATCC 31953, which has abnormal growth characteristics, which is in or on a biological medium suitable for growth of normal Neisseria gonorrhoeae, and observing for the restoration of normal growth to the abnormal growth strain Neisseria gonorrhorae ATCC 31953, in or on the biological medium of step (1), under conditions normal for growth of Neisseria gonorrhoeae. The observence of growth indicates positive detection of N. gonorrhoeae DNA.
Inventors
- 👤 Leonard J. Zubrzycki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 23:24
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/12; C12R; C12R2001/00; C12R2001/01; C12R2001/36; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/22; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
83 words
A biologically pure culture of the microorganism Neisseria gonorrhoeae ATCC 31953 capable of taking up gonococcal DNA, but which microorganism is abnormal in that it has characteristically poor growth on common chocolate agar at a temperature within the range of from about 30° C. to about 37° C. in a CO 2 atmosphere suitable for growth of normal Neisseria gonorrhoeae, and said microorganism having resistance to naldixic acid and streptomycin at the 5-10 mcg/ml level and the 1000 mcg/ml level or greater, respectively.
Claim 2
Dependent Claim
83 words
A biologically pure culture of the microorganism Neisseria gonorrhoeae ATCC 31953 capable of taking up gonococcal DNA, but which microorganism is abnormal in that it has characteristically poor growth on common chocolate agar at a temperature within the range of from about 30° C. to about 37° C. in a CO 2 atmosphere suitable for growth of normal Neisseria gonorrhoeae, and said microorganism having resistance to naldixic acid and streptomycin at the 5-10 mcg/ml level and the 1000 mcg/ml level or greater, respectively.
Claim 3
Dependent Claim
89 words
A biologically pure culture in accordance with claim 1 in the form of a preparation selected from the group consisting of (1) a fresh suspension in a buffer solution or a broth from growth of Neisseria gonorrhoeae ATCC 31953 on a solid medium, (2) a suspension in a buffer solution or a broth made from a lyophilized or dried preparation of Neisseria gonorrhoeae ATCC 31953, (3) a thawed suspension of Neisseria gonorrhoeae ATCC 31953 previously frozen to maintain viability, and (4) a broth culture of Neisseria gonorrhoeae ATCC 31953.
Claim 4
Dependent Claim
25 words
A biologically pure culture in accordance with claim 2 wherein the preparation contains an antibiotic at a final concentration tolerated by Neisseria gonorrhoeae ATCC 31953.
Claim 5
Independent Claim
97 words
The method for the laboratory diagnosis of gonorrhea by detecting DNA of Neisseria gonorrhoeae in a patient's specimen material, comprising the steps of (1) applying a non-toxic preparation of a patient's specimen material, directly to a culture of Neisseria gonorrhoeae ATCC 31953, which has abnormal growth characteristics, which is in or on a biological medium suitable for growth of normal Neisseria gonorrhoeae, and (2) observing for the restoration of normal growth to the abnormal growth strain Neisseria gonorrhoeae ATCC 31953, in or on the biological medium of step (1), under conditions normal for growth of Neisseria gonorrhoeae.
Claim 6
Independent Claim
97 words
The method for the laboratory diagnosis of gonorrhea by detecting DNA of Neisseria gonorrhoeae in a patient's specimen material, comprising the steps of (1) applying a non-toxic preparation of a patient's specimen material, directly to a culture of Neisseria gonorrhoeae ATCC 31953, which has abnormal growth characteristics, which is in or on a biological medium suitable for growth of normal Neisseria gonorrhoeae, and (2) observing for the restoration of normal growth to the abnormal growth strain Neisseria gonorrhoeae ATCC 31953, in or on the biological medium of step (1), under conditions normal for growth of Neisseria gonorrhoeae.
Claim 7
Dependent Claim
52 words
The method of claim 4 whereby the patient's specimen material is prepared by contacting it with a base to lyse Neisseria gonorrhoeae in the specimen material and then rendering the material non-toxic by the addition of an acid to adjust the pH to that which is tolerated by Neisseria gonorrhoeae ATCC 31953.
Claim 8
Dependent Claim
18 words
The method of claim 5 wherein the pH adjustment is done in the presence of a pH indicator.
Claim 9
Dependent Claim
26 words
The method of claim 5 wherein the base and acid both contain an antibiotic at a concentration tolerated by the test strain Neisseria gonorrhoeae ATCC 31953.
Claim 10
Dependent Claim
17 words
The method of claim 5 wherein the base and acid are prepared in a peptone containing buffer.
Claim 11
Dependent Claim
25 words
The method of claim 8 wherein the peptone containing buffer contains an antibiotic at a concentration tolerated by the test strain Neisseria gonorrhoeae ATCC 31953.
Claim 12
Dependent Claim
16 words
The method of claim 8 wherein both the base and acid may be inorganic or organic.
Claim 13
Dependent Claim
26 words
The method of claim 10 wherein the base is selected from the group consisting of NaOH, KOH or NH 4 OH and the acid is HCl.
Claim 14
Dependent Claim
43 words
The method of claim 4 wherein the preparation of a patient's specimen material is heated at a temperature from about 60° C. to about 75° C. for a time period from about ten minutes to about thirty minutes to inactivate any viable organisms.
Claim 15
Dependent Claim
33 words
The method of claim 4 wherein according to step (2) the restoration of normal growth to Neisseria gonorrhoeae ATCC 31953 is observed by the appearance of distinct visible colonies on a biological medium.
Claim 16
Dependent Claim
14 words
The method as in claim 13 wherein the biological medium is common chocolate agar.
Claim 17
Dependent Claim
27 words
The method as in claim 4 wherein the preparation of a patient's specimen material contains an antibiotic at a final concentration tolerated by Neisseria gonorrhoeae ATCC 31953.
Claim 18
Dependent Claim
157 words
A diagnostic test kit useful in the diagnosis of gonorrhea by detecting DNA of Neisseria gonorrhoeae in a patient's specimen material, the kit comprising: (1) a container with an aliquot of a test strain of Neisseria gonorrhoeae ATCC 31953 which microorganism is abnormal in that it has characteristically poor growth on common chocolate agar at a temperature within the range of from about 30° C. to about 37° C. in a CO 2 atmosphere suitable for growth of normal Neisseria gonorrhoeae, (2) a container with a solid or liquid medium capable of supporting growth of the test strain, as well as normal Neisseria gonorrhoeae, whereby, said aliquot can be applied to the solid or liquid medium and thereon exposed to a preparation of a patient's specimen material which is non-toxic to said abnormal test strain; the development of a normal growth of Neisseria gonorrhoeae colonies indicating the presence of Neisseria gonorrhoeae DNA in the patient's specimen material.
Claim 19
Dependent Claim
157 words
A diagnostic test kit useful in the diagnosis of gonorrhea by detecting DNA of Neisseria gonorrhoeae in a patient's specimen material, the kit comprising: (1) a container with an aliquot of a test strain of Neisseria gonorrhoeae ATCC 31953 which microorganism is abnormal in that it has characteristically poor growth on common chocolate agar at a temperature within the range of from about 30° C. to about 37° C. in a CO 2 atmosphere suitable for growth of normal Neisseria gonorrhoeae, (2) a container with a solid or liquid medium capable of supporting growth of the test strain, as well as normal Neisseria gonorrhoeae, whereby, said aliquot can be applied to the solid or liquid medium and thereon exposed to a preparation of a patient's specimen material which is non-toxic to said abnormal test strain; the development of a normal growth of Neisseria gonorrhoeae colonies indicating the presence of Neisseria gonorrhoeae DNA in the patient's specimen material.
References Cited (Backward Citations)
US3930956A
Cited
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