US4186182A
Expired / Lapsed
US
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Serological test for Neisseria gonorrhoeae antibodies
Assignee: Research Corp
•
Filing: 1977-09-07
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Granted: 1980-01-29
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Expires: 1997-09-07
Abstract
Abstract Serological method for determining presence of Neisseria gonorrhoeae antibodies in human sera and products utilized in such testing.
Inventors
- 👤 Hassan A. Gaafar
- 👤 Dora D'Arcangelis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 15:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; Y; Y10; Y10S; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
121 words
A serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises diluting the serum to be tested in physiological saline at a dilution of about 1:2 to 1:1000, heating the diluted serum at about 56° C. to 65° C. for from about 15 to 30 minutes and thereafter incubating with a composition containing a heat labile, species specific antigen produced from a growth culture of Neisseria gonorrhoeae to form an antigen-antibody conjugate when said antibodies are present and detecting the presence of said conjugate; the antigen being protein in nature and characterized as follows: 1. partially inactivated by heating at about 56° C. for about 30 minutes and completely inactivated by boiling for 1 h, 2.
Claim 2
Dependent Claim
121 words
A serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises diluting the serum to be tested in physiological saline at a dilution of about 1:2 to 1:1000, heating the diluted serum at about 56° C. to 65° C. for from about 15 to 30 minutes and thereafter incubating with a composition containing a heat labile, species specific antigen produced from a growth culture of Neisseria gonorrhoeae to form an antigen-antibody conjugate when said antibodies are present and detecting the presence of said conjugate; the antigen being protein in nature and characterized as follows: 1. partially inactivated by heating at about 56° C. for about 30 minutes and completely inactivated by boiling for 1 h, 2.
Claim 3
Dependent Claim
68 words
stable in an aqueous medium at pH values of 3-11, 3. stable when incubated with the following enzymes: deoxynucleic acidase ribonucleic acidase dextransase meuraminidase lysosyme, 4. inactivated when incubated with trypsin, 5. isoelectric point of 4±0.2, 6. molecular weight of active subunits of 37,000-40,000 as determined by SDS-polyacrylamide gel, 7. contains 1-10% carbohydrate and 2-3% organic phosphorus, and 8. soluble in acqueous media containing surface active agents, and
Claim 4
Dependent Claim
68 words
stable in an aqueous medium at pH values of 3-11, 3. stable when incubated with the following enzymes: deoxynucleic acidase ribonucleic acidase dextransase meuraminidase lysosyme, 4. inactivated when incubated with trypsin, 5. isoelectric point of 4±0.2, 6. molecular weight of active subunits of 37,000-40,000 as determined by SDS-polyacrylamide gel, 7. contains 1-10% carbohydrate and 2-3% organic phosphorus, and 8. soluble in acqueous media containing surface active agents, and
Claim 5
Dependent Claim
39 words
insoluble in methanol, chloroform, and acetone. 2. A serological method as in claim 1 wherein the presence of said conjugate is detected by reaction with an anti-human IgG labelled with a chemical which fluoresces when exposed to ultraviolet light.
Claim 6
Dependent Claim
20 words
A method as in claim 2 wherein the chemical is selected from the group consisting of fluorescein, rhodamine and auramine.
Claim 7
Dependent Claim
23 words
A method as in claim 2 wherein the antigen composition has an antigen unit value of at least 100 Ag. u./mg. of protein.
Claim 8
Dependent Claim
15 words
A method as in claim 2 wherein the antigen unit value is at least 1000.
Claim 9
Dependent Claim
20 words
A method as in claim 2 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21823.
Claim 10
Dependent Claim
20 words
A method as in claim 2 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21824.
Claim 11
Dependent Claim
20 words
A method as in claim 2 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21825.
Claim 12
Dependent Claim
25 words
A serological method as in claim 1 wherein the presence of said conjugate is detected by reaction with an antihuman IgG labelled with an enzyme.
Claim 13
Dependent Claim
32 words
A method as in claim 9 wherein the enzyme is selected from the group consisting of peroxidase, B-glucuronidase, B-D-glucosidase, B-D-galactosidase, urease, glucose oxidase plus peroxidase, galactose oxidase plus peroxidase, and acid phosphatase.
Claim 14
Dependent Claim
23 words
A method as in claim 9 wherein the antigen composition has an antigen unit value of at least 100 Ag. u./mg. of protein.
Claim 15
Dependent Claim
13 words
A method as in claim 9 wherein the value is at least 1000.
Claim 16
Dependent Claim
20 words
A method as in claim 9 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21823.
Claim 17
Dependent Claim
20 words
A method as in claim 9 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21824.
Claim 18
Dependent Claim
20 words
A method as in claim 9 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21825.
Claim 19
Dependent Claim
26 words
A serological method as in claim 1 wherein the presence of said conjugate is detected by reaction with an antihuman IgG labelled with a radioactive element.
Claim 20
Dependent Claim
26 words
A method as in claim 16 wherein the radioactive element is selected from the group consisting of 14 C, 125 I, 131 I, and 35 S.
Claim 21
Dependent Claim
20 words
A method as in claim 16 wherein the chemical is selected from the group consisting of fluorescein, rhodamine and auramine.
Claim 22
Dependent Claim
23 words
A method as in claim 16 wherein the antigen composition has an antigen unit value of at least 100 Ag. u./mg. of protein.
Claim 23
Dependent Claim
15 words
A method as in claim 16 wherein the antigen unit value is at least 1000.
Claim 24
Dependent Claim
20 words
A method as in claim 16 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21823.
Claim 25
Dependent Claim
20 words
A method as in claim 16 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21824.
Claim 26
Dependent Claim
20 words
A method as in claim 16 wherein the antigen is produced by a culture of Neisseria gonorrhoeae ATCC No. 21825.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-01-28 | 1983-07-30 | 1984-01-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-01-28 | 1987-07-30 | 1988-01-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-01-28 | 1991-07-30 | 1992-01-29 | $7700.00 | Window Closed / Lapsed |